Abstract
In 75 male and 46 female subjects of an urban population (93% Russians) and in 38 males and 40 females of a rural population (87% Russians), the antioxidant activity (AOA) of blood plasma was determined from the plasma ability to reduce the yield of products interacting with thiobarbituric acid in the model lecithin–Fe2+ ion system. In the urban population, the loci TF(AvaI in exon5) and ACE (I/D polymorphism of the Alu repeat in intron16) were studied in 130 and 141 subjects, respectively. Of them, 102 and 111 subjects, respectively, were examined for AOA. In the rural population, the corresponding sample sizes were 75 and 76 (73 and 74 subjects were examined for AOA). The polymorphic loci of the urban and rural populations did not differ in the allele frequencies. In both populations Hardy–Weinberg and gametic equilibria were observed. The contributions of the TF and ACE genes to AOA variation in the combined sample from the urban and rural populations were 0.6 and 0.5%, respectively.
Similar content being viewed by others
References
Velichkovskii, B.T., Free Radical-Mediated Oxidation as a Step in Immediate and Long-Term Adaptation of the Organism to Environmental Factors, Vestn. Rus. Akad. Med. Nauk, 2001, no. 6, pp. 45-52.
ladimirov, Yu.A., Free Radicals and Antioxidants, Vestn. Rus. Akad. Med. Nauk, 1998, no. 7, pp. 43-51.
Mates, J.M. and Sanches-Jimenes, F.M., Role of Reactive Oxygen Species in Apoptosis: Implications for Cancer Therapy, Int. J. Biochem. Cell Biol., 2000, vol. 32, no. 2, pp. 157-170.
Lenaz, G., Role of Mitochondria in Oxidative Stress and Aging, Biochim. Biophys. Acta, 1998, vol. 1366, nos. 1-2, pp. 53-67.
Halliwell, B., Free Radicals, Antioxidants, and Human Disease: Curiosity, Cause, or Consequence?, Lancet, 1994, vol. 344, pp. 721-724.
Dubinina, E.E., The Antioxidant System of Blood Plasma, Ukr. Bot. Zh., 1992, vol. 64, no. 2, pp. 3-15.
Zborovskaya, I.A. and Bannikova, M.V., The Antioxidant System of the Organism, Its Role in Metabolism, and Clinical Aspects, Vestn. Rus. Akad. Med. Nauk, 1995, no. 6, pp. 53-59.
Zhuravlev, A.I., Bioantioxidants in the Animal Organism, Tr. Mosk. O-va Ispyt. Prir., 1982, vol. 52, pp. 15-29.
Halliwell, B. and Gutteridge, J.M.C., The Antioxidants of Human Extracellular Fluids, Arch. Biochem. Biophys., 1990, vol. 280, no. 1, pp. 1-8.
Berry, E.M. and Kohen, R., Is the Biological Antioxidant System Integrated and Regulated?, Med. Hypotheses, 1999, vol. 53, no. 5, pp. 397-401.
Lewin, G. and Popov, I., The Antioxidant System of the Organism: Theoretical Basis and Practical Consequences, Med. Hypotheses, 1994, vol. 42, no. 4, pp. 269-275.
Zaitsev, V.G. and Zakrevskii, V.I., Technical Aspects of Studies of Free Radical-Mediated Oxidation and the Antioxidant System of the Organism, Vestn. Volgograd. Med. Akad., 1998, no. 4, pp. 49-53.
Klebanov, G.I., Teselkin, Yu.O., Babenkova, I.V., et al., The Antioxidant Activity of Serum, Vestn. Rus. Akad. Med. Nauk, 1999, no. 2, pp. 15-22.
Cao, G. and Prior, R.L., Comparison of Different Analytical Methods for Assessing Total Antioxidant Capacity of Human Serum, Clin. Chem., 1998, vol. 44, no. 6, pp. 1309-1315.
Wang, X.L., Rainwater, D.L., VandeBerg, J.F., et al., Genetic Contributions to Total Plasma Antioxidant Activity, Arterioscler. Thromb. Vasc. Biol., 2001, vol. 21, no. 7, pp. 1102-1103.
Marussin, A.V., Puzyrev, V.P., and Bragina, E.Yu., Population Genetic Analysis of the Variation in Antioxidant Activity of Human Plasma, Sib. Med. Zh., 2001, vol. 16, no. 1, pp. 41-46.
Eliseeva, Yu.E., Angiotensin-Converting Enzyme and Its Physiological Role, Vopr. Med. Khim., 2001, vol. 47, no. 1, pp. 43-54.
Griendling, K.K., Minieri, C.A., Ollerenshaw, J.D., and Alexander, R.W., Angiotensin II Stimulates NADH and NADPH Oxidase Activity in Cultured Vascular Smooth Muscle Cells, Circ. Res., 1994, vol. 74, no. 6, pp. 1141-1148.
Rajagopalan, S., Kurz, S., Munzel, T., et al., Angiotensin II-Mediated Hypertension in the Rat Increases Vascular Superoxide Production via Membrane NADH/NADPH Oxidase Activation: Contribution to Alterations of Vasomotor Tone, J. Clin. Invest., 1996, vol. 97, no. 8, pp. 1916-1923.
Faure-Delanef, L., Baudin, B., Beneteau-Burnat, B., et al., Plasma Concentration, Kinetic Constants, and Gene Polymorphism of Angiotensin I-Converting Enzyme in Centenarians, Clin. Chem., 1998, vol. 44, no. 10, pp. 2083-2087.
Rigat, B., Hubert, C., Alhenc-Gelas, F., et al., An Insertion/Deletion Polymorphism in the Angiotensin I-Converting Enzyme Gene Accounting for Half the Variance of Serum Enzyme Levels, J. Clin. Invest., 1990, vol. 86, pp. 1343-1346.
Semenova, I.V., Ernestova, L.S., Konev, V.V., and Ryabchenko, N.I., Employment of the Lipid Peroxidation Technique in Biotesting Waters, Gig. Sanit., 1997, no. 1, pp. 51-52.
Kutmin, A.I. and Marussin, A.V., The Antioxidant Activity of Plasma in People Exposed to Chronic Low-Ionizing Irradiation, Byull. Eksp. Biol. Med., 1999, vol. 127, pril. 1, pp. 34-37.
Johns, M. and Paulus-Thomas, J., Purification of Human Genomic DNA from Whole Blood Using Sodium Perchlorate in Place of Phenol, Anal. Biochem., 1989, vol. 80, no. 2, pp. 276-278.
Beckman, L.E., Van Landeghem, G.F., Sikstrom, C., and Beckman, L., DNA Polymorphisms and Haplotypes in the Human Transferrin Gene, Hum. Genet., 1998, vol. 102, no. 2, pp. 141-144.
Spiridonova, M.G., A Molecular Genetic Study of Susceptibility to Coronary Atherosclerosis, Cand. Sci. (Biol.) Dissertation, Tomsk, 1999.
Rigat, B., Hubert, C., Corvol, P., and Soubrier, F., PCR Insertion/Deletion Polymorphism of the Human Angiotensin-Converting Enzyme Gene (DCP1) (Dipeptidyl Carboxypeptidase), Nucleic Acids Res., 1992, vol. 20, p. 1433.
Lakin, G.F., Biometriya (Biometrics), Moscow: Vysshaya Shkola, 1990.
Sing, C.F. and Davignon, J., Role of the Apolipoprotein E Polymorphism in Determining Normal Plasma Lipid and Lipoprotein Variation, Am. J. Hum. Genet., 1985, vol. 37, no. 2, pp. 268-285.
Boerwinkle, E. and Sing, C.F., Bias of the Contribution of Single-Locus Effects to the Variance of a Quantitative Trait, Am. J. Hum. Genet., 1986, vol. 39, no. 1, pp. 137-144.
Zhivotovsky, L.A., Populyatsionnaya biometriya (Population Biometry), Moscow: Nauka, 1991.
Blumenthal, M.N., Namboodiri, K.K., Mendell, N., et al., Genetic Transmission of Serum IgE Levels, Am. J. Med. Genet., 1981, vol. 10, pp. 219-228.
Bankova, V.V., Nikanorova, T.M., Polyakov, S.D., and Tagieva, T.A., Malonic Aldehyde Degradation and Its Age-Dependent, Seasonal, and Daily Changes, Vopr. Med. Khim., 1988, vol. 34, no. 6, pp. 27–30.
Van de Vijver, L.P., Van Duyvenvoorde, W., Buytenhek, R., et al., Seasonal Variation in Low Density Lipoprotein Oxidation and Antioxidant Status, Free Radic. Res., 1997, vol. 27, no. 1, pp. 89-96.
Sokolovskii, V.V., Thiole Antioxidants in Molecular Mechanisms of the Nonspecific Response of the Organism to Extreme Factors: A Review, Vopr. Med. Khim., 1988, vol. 34, no. 6, pp. 2-11.
Stoneking, M., Fontius, J.J., Clifford, S.L., et al., Alu Insertion Polymorphisms and Human Evolution: Evidence for a Larger Population Size in Africa, Genome Res., 1997, vol. 7, no. 11, pp. 1061-1071.
Foy, C.A., McCormack, L.J., Knowler, W.C., et al., The Angiotensin I-Converting Enzyme (ACE) Gene I/D Polymorphism and ACE Levels in Pima Indians, J. Med. Genet., 1996, vol. 33, no. 4, pp. 336-337.
Miloserdova, O.V., Slominsky, P.A., Mauyanov, I.V., et al., Association between the Insertion/Deletion Polymorphism of the Angiotensin-Converting Enzyme Gene and Angiopathies in Patients with Insulin-Independent Diabetes Mellitus from the Chuvash Republic, Genetika (Moscow), 2001, vol. 37, no. 1, pp. 112-116.
Dolgikh, M.M., Voevoda, M.I., Malyutina, S.K., et al., Population Frequencies and Age-Dependent Dynamics of Genotypes and Alleles of the Angiotensin-Converting Enzyme Gene in the Novosibirsk Population, Bioraznoobrazie i dinamika ekosistem Severnoi Evrazii: informatsionnye tekhnologii i modelirovanie: Tezisy dokladov I mezhdunarodnogo rabochego soveshchaniya (Biodiversity and Dynamics of North Eurasian Ecosystems: Information Technologies and Modeling: Proc. I Int. Workshop, Novosibirsk, 2001, p. 108.
Lee, P.L., Ho, N.J., Olson, R., and Beutler, E., The Effect of Transferrin Polymorphisms on Iron Metabolism, Blood Cells Mol. Dis., 1999, vol. 25, nos. 5-6, pp. 374-379.
Blanche, H., Cabanne, L., Sahbatou, M., and Thomas, G., A Study of French Centenarians: Are ACE and APOE Associated with Longevity?, C. R. Acad. Sci. III, 2001, vol. 324, no. 2, pp. 129-135.
Rossi, G.P., Taddei, S., Virdis, A., et al., Exclusion of the ACE I/D Polymorphism as a Determinant of Endothelial Dysfunction, Hypertension (Dallas), 2001, vol. 37, no. 2, pp. 293-300.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marussin, A.V., Puzyrev, V.P., Salyukov, V.B. et al. Correlation between Polymorphism of the Genes for Transferrin and Angiotensin-Converting Enzyme and Antioxidant Activity of Blood Plasma. Russian Journal of Genetics 39, 700–705 (2003). https://doi.org/10.1023/A:1024422330142
Issue Date:
DOI: https://doi.org/10.1023/A:1024422330142