Although common carp is the major fish species in Asian and European aquaculture and many domestic varieties have occurred, there is a controversy about the origination of European domestic common carp. Some scientists affirmed that the ancestor of European domestic common carp was Danube River wild common carp, but others considered it might be Asian common carp. For elucidating origination of European domestic common carp, we chose two representative European domestic common carp strains (German mirror carp and Russian scattered scaled mirror carp) and one wild common carp strain of Cyprinus carpio carpio subspecies (Volga River wild common carp) and two Asian common carp strains, the Yangtze River wild common carp (Cyprinus carpio haematopterus) and traditionally domestic Xingguo red common carp, as experimental materials. ND5–ND6 and D-loop segments of mitochondrial DNA were amplified by polymerase chain reaction and analyzed through restriction fragment length polymorphism (RFLP) and sequencing respectively. The results revealed that HaeIII and DdeI digestion patterns of ND5–ND6 segment and sequences of control region were different between European subspecies C. carpio carpio and Asian subspecies C. carpio haematopterus. Phylogenetic analysis showed that German mirror carp and Russian scattered scaled mirror carp belonged to two subspecies, C. carpio carpio and C. carpio haematopterus, respectively. Therefore, there were different ancestors for domestic carp in Europe: German mirror carp was domesticated from European subspecies C. carpio carpio and Russian scattered scaled mirror carp originated from Asian subspecies C. carpio haematopterus.
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Avise, J.C., 1994. Molecular Markers, Natural History and Evolution. Chapman & Hall, New York.
Balon, E.K., 1995. Origin and domestication of the wild carp, Cyprinus carpio: from Roman gourmets to the swimming flowers. Aquaculture 129: 3–47.
Chang, Y.S., F.L. Huang & T.B. Lo, 1994. The complete nucleotide sequence and gene organization of carp (Cyprinus carpio) mitochondrial genome. J. Mol. Evol. 38: 138–155.
Chiba, T., N. Kurihama & Y. Kenichi, 1966. Culture of Common Carp. Green Book House Publisher, Tokyo.
Cronin, M.A., W.J. Spearman, R.L. Wilmot, J.C. Patton & J.W. Bickham, 1993. Mitochondrial DNA variation in Chinook (Oncorhynchus tshawytscha) and chum salmon (O. keta) detected by restriction enzyme analysis of polymerase chain reaction (PCR) products. Can. J. Fish. Aquat. Sci. 50: 708–715.
Gross, R., K. Kohlmann & P. Kersten, 2002. PCR-RFLP analysis of the mitochondrial DNA ND-3/4 and ND-5/6 gene polymorphisms in the European and East Asian subspecies of common carp (Cyprinus carpio L.). Aquaculture 204: 507–516.
Hickling, C., 1962. Fish Culture. Faber and Faber, London.
Higgins, D.G. & P.M. Sharp, 1988. CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73: 237–244.
Higgins, D.G. & P.M. Sharp, 1989. Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5: 151–153.
Kanda, N. & F.W. Allendorf, 2001. Genetic population structure of bull trout from the Flathead River basin as shown by microsatellites and mitochondrial DNA markers. Trans. Amer. Fish. Soc. 130: 92–102.
Kirpichnikov, V.S., 1981. Genetic Bases of Fish Selection (Translated by G.G. Gause). Springer-Verlag, Berlin.
Kohlmann, K. & P. Kersten, 1999. Genetics variability of German and foreign common carp (Cyprinus carpio L.) population. Aquaculture 173: 435–445.
Komen, J., 1990. Clones of common carp, Cyprinus carpio. PhD Thesis, Wageningen Agricultural University.
Kumar, S., K. Tamura, I.B. Jakobsen & M. Nei, 2001. MEGA 2: Molecular Evolutionary Genetics Analysis Software, Arizona State University, Tempe, AZ, USA.
Liu, H.Z., C.S. Tzeng & H.Y. Teng, 2002. Sequence variations in the mitochondrial DNA control regions and their implication for the phylogeny of the Cypriniformes. Can. J. Zool. 80: 569–581.
McElroy, D., P. Moran, E. Bermingham & I. Kornfield, 1992. REAP: an integrated environment for the manipulation and phylogenetic analysis of restriction data. J. Hered. 83: 157–158.
Murakami, M., Y. Yamashita & H. Fujitani, 1998. The complete sequence of mitochondrial genome from a gynogenetic triploid 'ginbuna' (Carassius auratus langsdorfii). Zool. Sci. 15: 335–337.
Nei, M. & W.H. Li, 1979. Mathematical model for studying genetic variant in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269–5273.
Nei, M., 1987. Molecular Evolutionary Genetics. Columbia University Press, New York.
Nielsen, J., M.C. Fountain, J.C. Favela, K. Cobble & B.L. Jensen, 1998. Oncorhynchus at the southern extent of their range: a study of mtDNA control region sequence with special reference to an undescribed subspecies of O. mykiss from Mexico. Environ. Biol. Fish. 51: 7–23.
Ong, T.L., J. Stabile & I. Wirgin, 1996. Genetic divergence between Acipenser oxyrinchus and A. o. desotoi as assessed by mitochondrial DNA sequencing analysis. Copeia 2: 464–469.
Park, K.L., M.A. Brainard, D.A. Dightman & G.A. Winans, 1993. Low levels of intraspecific variation in the mitochondrial DNA of chum salmon (Onchorhynchus keta). Mol. Mar. Biol. Biotechnol. 2: 362–370.
Sambrook, J., E.F. Fritsch & T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 2nd edn.
Tong, J.G. & Q.J. Wu, 2001. Sequence conservation on segments of mitochondrial 16S rRNA and cytochrome b in strains of common carp (Cyprinus carpio). Acta Hydrobiol. Sinica 25: 54–60.
Vooren, C.M., 1972. Ecological aspects of the introduction of fish species into natural habitats in Europe, with special reference to the Netherlands. J. Fish Biol. 4: 565–583.
Wohlfarth, G., 1984. Common carp, in Evolution of Domesticated Animals, edited by I.L. Mason. Longman, London.
Wu Xianwen et al., 1977. Monographs of Cyprininae in China. Shanghai People's Publisher, Shanghai.
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Zhou, J.F., Wu, Q.J., Ye, Y.Z. et al. Genetic Divergence Between Cyprinus carpio carpio and Cyprinus carpio haematopterus as Assessed by Mitochondrial DNA Analysis, with Emphasis on Origin of European Domestic Carp. Genetica 119, 93–97 (2003). https://doi.org/10.1023/A:1024421001015
- control region
- Cyprinus carpio
- domestic carp origin