Skip to main content
SpringerLink
Log in

Effects of Pentoxifylline on Alcohol-Induced Gastric Injury and Acid Secretion in Rats

  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

We aimed to evaluate the protective effects of pentoxifylline on alcohol-induced gastric injury, its relation with nitric oxide and prostaglandin synthesis, as well as gastric acidity in rats. Acute gastric mucosal injury was induced by intragastric infusion of 2 ml 98% alcohol. Pentoxifylline was given at 100 mg/kg intraperitoneally. Indomethacin and NG-nitro-L arginine were used to inhibit prostaglandin and nitric oxide synthesis, respectively. Macroscopic and microscopic gastric injuries were evaluated. Gastric pH, tissue malondialdehyde levels, oxidized and reduced glutathion (GSSG/GSH) levels, and effects of pentoxifylline on gastric acid output were measured. Pentoxifylline pretreatment significantly reduced macroscopic and microscopic gastric injury. Malondialdehyde level was lower in pentoxifylline treated rats (351.1 ± 94.1 nmol/g vs 624.3 ± 234.2 nmol/g). Pentoxifylline has a protective role on alcohol-induced gastric mucosal injury in rats. This effect is not related to synthesis of prostaglandins and changes in gastric acidity but does seem to be related to nitric oxide-mediated pathways. In contrast, pentoxifylline increases gastric acid output significantly.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ward A, Clissold S: Pentoxifylline: a review of its pharmacodynamic and pharmacokinetic properties, and its therapeutic efficacy. Drugs 34:50-97, 1987

    Google Scholar 

  2. Samlaska P, Winfield EA: Pentoxifylline. J Am Acad Dermatol 30:603-621, 1994

    Google Scholar 

  3. Ely H: Pentoxifylline therapy in dermatology: a review of localized hyperviscosity and its effects on the skin. Dermatol Clin 6:585-608, 1988

    Google Scholar 

  4. Tominaga G, Waxman K, Soliman H, Sarfeh J: Protective effect of pentoxifylline on gastric mucosa. J Surg Res 44:727-732, 1988

    Google Scholar 

  5. Santucci L, Fioricci S, DiMatteo FM, Morelli A: Role of tumour necrosis factor alpha release and leukocyte margination in indomethacin-induced gastric injury in rats. Gastroenterology 108:393-401, 1995

    Google Scholar 

  6. Robert A: Cytoprotection by prostaglandins. Gastroenterology 77(4):761-767, 1979

    Google Scholar 

  7. Wallace JL: Increased resistance of the rat gastric mucosa to hemorrhagic damage after exposure to an irrtant. Role of the mucoid cap and prostaglandin synthesis. Gastoenterology 94:22-32, 1988

    Google Scholar 

  8. Tache Y, Saperas E: Potent inhibition of gastric acid secretion and ulcer formation by centrally and peripherally administered interleukin-1. Ann NY Acad Sci 664:353-368, 1992

    Google Scholar 

  9. Lacy ER, Ito S: Microscopic analysis of ethanol damage to rat gastric mucosa after treatment with a prostaglandin. Gastroenterology 83:619-625, 1982

    Google Scholar 

  10. Ohkawa H, Ohishi N, Yagi K: Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351-358, 1979

    Google Scholar 

  11. Rungby J, Flyvbierg A, Andersen H, Nyborg K: Lipid peroxidation in early experimental diabetes in rats: effect of diabetes and insulin. Acta Endocrinol 126:378-380, 1992

    Google Scholar 

  12. Teare JP, Punchard PA, Powell P, Lumb J: Automated spectrophotometric method for determining oxidized and reduced glutathione in liver. Clin Chem 39(4):686-689, 1993

    Google Scholar 

  13. Griffith OW: Determination of glutathion and glutathion disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106:207-212, 1980

    Google Scholar 

  14. Yamamato O, Okada Y, Okabe S: Effects of proton pomp inhibitor, omeprazole, on gastric secretion and gastric and duedonal ulcer or erosions in rats. Dig Dis Sci 29:394-401, 1984

    Google Scholar 

  15. Salim AWS: Role of oxygen derived free radicals in mechanism of acute and chronic duedonal ulceration in the rat. Dig Dis Sci 35:73-79, 1990

    Google Scholar 

  16. Szelenyi I, Brune K: Possible role of free radicals in ethanol-induced gastric mucosal damage in rats. Dig Dis Sci 33:865-871, 1988

    Google Scholar 

  17. Steuin HS, Esplugues J, Whittle B: Direct cytotoxic effects of oxygen radicals on the gastric mucosa. Surgery 106:318-324, 1989

    Google Scholar 

  18. Mutoh H, Hiraishi H, Ota S, Ivey K, Terano A: Role of oxygen radicals in ethanol-induced damage to cultured gastric mucosal cells. Am J Physiol 258:603-609, 1990

    Google Scholar 

  19. Mutoh H, Hiraishi H, Ota S, Yoshida H, Ivey K, Terano A: Protective role of intracellular glutathione against ethanol induced damage to cultured gastric mucosal cells. Gastroenterology 98:1452-1459, 1990

    Google Scholar 

  20. Bou-Abbud CF, Wayland H, Paulsen G, Guth PH: Microcirculatory stasis precedes tissue necrosis in ethanol induced gastric mucosal injury. Dig Dis Sci 33:872-877, 1988

    Google Scholar 

  21. Pihan G, Regillo BA, Szabo MD: Free radical and lipid peroxidation in ethanol or aspirin induced gastric mucosal injury. Dig Dis Sci 32(12):1395-1401, 1987

    Google Scholar 

  22. Szabo S, Trier JS, Brown A, Schnoor J. Early vascular and increased vascular permeability in gastric mucosal injury caused by ethanol in the rat. Gastroenterology 88:228-236, 1985

    Google Scholar 

  23. Robert A, Eberle D, Kaplowitz N: Role of glutathione in gastric mucosal cytoprotection. Am J Physiol 247:296-304, 1984

    Google Scholar 

  24. Mandell G: Cytokines, phagocytes, and pentoxifylline. J Cardiovas. Pharmacol 258(2):20-22, 1995

    Google Scholar 

  25. Monacado S, Palmer RM, Higgs EA: Nitric oxide physiology pathophysiology and pharmacology. Pharmacol Rev 43:109-142, 1991

    Google Scholar 

  26. Casadevall M, Panes J, Pigue JM, Maroni N, Bosch J, Whittle BJR: Involvement of nitric oxide and prostaglandin's in gastric mucosal hyperemia of portal hypertensive anesthesized rats. Hepatology 18:628-634, 1993

    Google Scholar 

  27. Santucci L, Fioricci S, Giansanti M, Brunori PM: Pentoxifylline prevents indomethacin-induced gastric mucosal damage in rats: role of tumour necrois factor alpha. Gut 35(7):909-915, 1994

    Google Scholar 

  28. Karaoýlu Aö, Kösekli MA, özütemiz ö, Erkuß M, ünal E, Batur Y: The effects of pentoxifylline, omeprazole, pentoxifylline and omeprazole on ethanol-induced gastric mucosal injury in rats. Turk J Gastroenterol 10(2):20-25, 1999

    Google Scholar 

  29. Rieneck K, Diamant M, Haahr PM, Schonharting M, Bendtzen K: In vitro immunomodulatory effects of pentoxifylline. Immunol Lett 37:131-138, 1993

    Google Scholar 

  30. Beales IL, Calam J: Interleukin 1 beta and tumour necrosis factor alpha inhibit acid secretion in cultured rabbit parietal cells by multiple pathways. Gut 42(2):227-234, 1998

    Google Scholar 

  31. Saperas E, Tache Y: Central interleukin-1 beta-induced inhibition of acid secretion in rats: specificity of action. Life Sci 52(9):785-792, 1993

    Google Scholar 

Download references

Authors
  1. Nadir Yönetci
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mehmet Ali Kösekli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Ömer Özütemiz
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ali Önder Karaoğlu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nevin Oruç
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muhan Erkuş
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Tijen Tanyalçin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yücel Batur
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yönetci, N., Kösekli, M.A., Özütemiz, A.Ö. et al. Effects of Pentoxifylline on Alcohol-Induced Gastric Injury and Acid Secretion in Rats. Dig Dis Sci 48, 1392–1396 (2003). https://doi.org/10.1023/A:1024290615796

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1024290615796

Search

Navigation