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Multitasking with molecular dynamics Typhoon: quantifying nucleic acids and autoradiographs

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Abstract

With increased sensitivity and specificity, fluorescent assays are rapidly becoming the method of choice for nucleic acid quantification. The utility of the Typhoon scanner has now been extended to accurately measure low levels of DNA and RNA (5 ng ml−1) with PicoGreen and RiboGreen dyes. In addition, with a few simple modifications, autoradiographic film images can be scanned and quantified with the Typhoon series of scanners.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology and Center for Molecular Medicine and Genetics, Wayne State University, Detroit, MI~, 48201, USA

    Robert J. Goodrich & G. Charles Ostermeier

  2. Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics and Center for Scientific Computing, Wayne State University, Detroit, MI~, 48201, USA

    Stephen A. Krawetz

Authors
  1. Robert J. Goodrich
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  2. G. Charles Ostermeier
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  3. Stephen A. Krawetz
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Correspondence to Stephen A. Krawetz.

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Goodrich, R.J., Ostermeier, G.C. & Krawetz, S.A. Multitasking with molecular dynamics Typhoon: quantifying nucleic acids and autoradiographs. Biotechnology Letters 25, 1061–1065 (2003). https://doi.org/10.1023/A:1024154817579

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  • DOI: https://doi.org/10.1023/A:1024154817579

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