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Dual Effects of Endogenous DNases on Transcriptionally Active and Inactive Chromatin

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Abstract

Experiments on resting hepatocytes with inactive c-fos gene and active albumin gene. We revealed that DNA of the transcribed gene is less susceptible to the influence of endogenous Ca2+/Mg2+-dependent DNases in matrix-associated and highly soluble chromatin fractions. In the fraction of low soluble chromatin active gene was more accessible for DNases. Our results indicate that activity of endogenous DNases can change in the transcribed gene locus.

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Authors and Affiliations

  1. Russian Academy of Sciences, Laboratory of Molecular Biology, Institute for Problem of Chemical Physics, Chernogolovka, Moscow region

    N. P. Borisova, G. V. Kostyuk, N. A. Shevchenko, P. Ya. Boikov, E. V. Rudakova, R. I. Papina & A. A. Terent'ev

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  1. N. P. Borisova
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  2. G. V. Kostyuk
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  3. N. A. Shevchenko
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  4. P. Ya. Boikov
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  5. E. V. Rudakova
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  6. R. I. Papina
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  7. A. A. Terent'ev
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Correspondence to A. A. Terent'ev.

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Borisova, N.P., Kostyuk, G.V., Shevchenko, N.A. et al. Dual Effects of Endogenous DNases on Transcriptionally Active and Inactive Chromatin. Bulletin of Experimental Biology and Medicine 135, 253–257 (2003). https://doi.org/10.1023/A:1024132930490

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  • DOI: https://doi.org/10.1023/A:1024132930490

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