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Evaluation of the phytic acid effect on the labeling of blood elements with technetium-99m and on the survival of a strain of Escherichia coli treated with stannous fluoride

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Abstract

The labeling of red blood cells with technetium-99m(99mTc) depends on a reducing agent and stannous ions, as chloride or fluoride, are widely utilized. This labeling may also be altered by drugs. Moreover, some authors have reported that the survival of Escherichia coli (E. coli) cultures decreases in presence of stannous ions. Phytic acid is present in the daily diet and we evaluated its influence on: (i) the labeling of blood elements with 99mTc and (ii) on the survival of an E. coli strain treated with stannous fluoride. Heparinized whole blood was withdrawn from Wistar rats and it was incubated with stannous chloride and with 99mTc, as sodium pertechnetate, centrifuged and plasma (P) and blood cells (BC) were isolated. Samples of P and BC were also precipitaded with trichloroacetic acid, centrifuged and soluble (SF) and insoluble fractions (IF) isolated. E. coli culture was treated with stannous fluoride in presence of phytic acid. As phytic acid altered the fixation of 99mTc on BC, on IF-P and on IF-BC and, moreover, it abolished the lethal effect of stannous fluoride on the E. coli culture, we can suggest that, probably, phytic acid would have chelating properties to the stannous ions.

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References

  1. Raboy V: Seeds for a better future: ‘low phytate’ grains help to overcome malnutrition and reduce pollution. Trends Plant Sci 6: 458-462, 2001

    Google Scholar 

  2. Shears SB: Assessing the omnipotence of inositol hexakisphosphate. Cell Signal 13: 151-158, 2001

    Google Scholar 

  3. Grases F, Simonet BM, Prieto RM, March JG: Phytate levels in diverse rat tissues: Influence of dietary phytate. Br J Nutr 86: 225-231, 2001

    Google Scholar 

  4. Braga ACS, Oliveira MBN, Feliciano GD, Reiniger IW, Oliveira JF, Silva CR, Bernardo-Filho M: The effect of drugs on the labeling of blood elements with technetiun-99m. Curr Pharm Des 6: 1179-1191, 2000

    Google Scholar 

  5. Melo SF, Soares SF, Costa RF, Silva CR, Oliveira MB, Bezerra RJ, Caldeira-de-Araújo A, Bernardo-Filho M: Effect of Cymbopogon citratus, Maytenus ilicifolia and Baccharis genistelloides extracts against the oxidative damage caused by stannous chloride in Escherichia coli. Mutat Res 496: 33-38, 2001

    Google Scholar 

  6. Mattos DMM, Gomes ML, Freitas RS, Bernardo-Filho M: Model to evaluate the toxic effect of drugs: Vincristine effect in the mass of organs and in the distribution of radiopharmaceuticals in mice. Mutat Res 496: 137-143, 2001

    Google Scholar 

  7. Gomes ML, Braga ACS, Mattos DMM, Freitas RS, Paula EF, Bezerra RJAC, Bernardo-Filho M: Effect of mitomycin-C on the bioavailability of the radiopharmaceutical 99mTechnetium-phytic acid in mice: A model to evaluate the toxicological effect of a chemial drug. J Appl Toxicol 22: 85-87, 2002

    Google Scholar 

  8. Hladik WB III, Saha GB, Study KT: Essentials of Nuclear Medicine Science. Williams and Wilkins, Baltimore, MD, London, 1987

    Google Scholar 

  9. Saha GB: Fundamentals of Nuclear Pharmacy, 4th Edit. Springer-Verlag, New York, 1998

    Google Scholar 

  10. Early PJ, Sodee DB: Principles and Practice of Nuclear Medicine, 2nd Edit. Mosby, Toronto, 1995

    Google Scholar 

  11. Bernardo-Filho M, Silva JRM, Reis RJN, Boasquevisque EM, Hassón-Voloch A: Conditions for labeling of Schistosoma mansoni cercaria with technetium-99m. J Nucl Biol Med 36: 56-59, 1992

    Google Scholar 

  12. Bernardo-Filho M, Pires ET, Boasquevisque EM, Hassón-Voloch A: Studies on the incorporation of 99m-technetium to the platyhelminth Dugesia tigrina. Riv Parassitol XIIV: 7-11, 1993

    Google Scholar 

  13. Gutfilen B, Marinho JCA, Rozemblum S, Bernardo-Filho M: 99mTclabeled leukocytes obtained with a suitable technique: The biodistribution study in rabbits. Acta Med Biol 41: 193-196, 1993

    Google Scholar 

  14. Plotkowski MC, Bernardo-Filho M, Meirelles MN, Tournier JM, Puchelle E: Pseudomonas aeruginosa binds to soluble cellular fibronectin. Curr Microbiol 26: 91-95, 1993

    Google Scholar 

  15. Harbert JC, Eckelman WC, Neumann RD: Nuclear Medicine Diagnosis and Therapy. Thieme, New York, 1996

    Google Scholar 

  16. Bernardo-Filho M, Gutfilen G, Macil OS: Effect of different anticoagulants on the labeling of red blood cells and plasma proteins with Tc-99m. Nucl Med Commun 15: 730-734, 1994

    Google Scholar 

  17. Hesslewood S, Leung E: Drug interactions with radiopharmaceuticals. Eur J Nucl Med 21: 348-356, 1994

    Google Scholar 

  18. Sampson CB: Complications and difficulties in radiolabelling blood cells: A review. Nucl Med Commun 17: 648-658, 1996

    Google Scholar 

  19. Oliveira JF, Braga ACS, Ávila ASR, Gutfilen B, Bernardo-Filho M: Effect of Thuya occidentalis on the labeling of red blood cells and plasma proteins with technetium-99m. Yale J Biol Med 69: 489-494, 1997

    Google Scholar 

  20. Vidal MV, Gutfilen B, Barbosa-da-Fonseca LM, Bernardo-Filho M: Influence of tobacco on the labeling of red blood cells and plasma proteins with technetium-99m. J Exp Clin Cancer Res 17: 1-6, 1998

    Google Scholar 

  21. Oliveira JF, Braga ACS, Ávila ASR, Caldeira-de-Araújo A, Cardoso VN, Bezerra RJAC, Bernardo-Filho M: Assessment of the effect of Maytenus ilicifolia (espinheira santa) extract on the labeling of red blood cells and plasma proteins with technetium-99m. J Ethnopharmacol 72: 179-184, 2000

    Google Scholar 

  22. Gutfilen B, Boasquevisque EM, Bernardo-Filho M: Calcium channel blockers: Interference on red blood cells and plasma proteins labeling with 99mTc. Rev Esp Med Nucl 11: 195-199, 1992

    Google Scholar 

  23. Hallas LE, Cooney JJ: Tin and tin-resistante microorganisms in Chesapeake Bay. Appl Environ Microbiol 41: 466-471, 1981

    Google Scholar 

  24. McLean JRN, Blakey DH, Douglas GR, Kaplan JR: The effect of stannous and stannic (tin) chloride on DNA in Chinese hamster ovary cells. Mutat Res 119: 195-201, 1983

    Google Scholar 

  25. Rader JI: Anti-nutritive effects of dietary tin. Adv Exp Med Biol 289: 509-524, 1991

    Google Scholar 

  26. White DJ: Return to stannous fluoride dentifrices. J Clin Dentol VI(Spec Issue): 29-36, 1995

    Google Scholar 

  27. Budavery S (ed): The Merck Index. Merck, White-house Station, NJ, 1996, pp 1500-1501

    Google Scholar 

  28. Caldeira-de-Araújo A, Dantas FJS, Moraes MO, Felzenszwalb I, Bernardo-Filho M: Stannous chloride participates in the generation of reactive oxygen species. Ciênc Cult J Brazil Assoc Adv Sci 48: 109-113, 1996

    Google Scholar 

  29. Vasconcelos BHV, Bernardo-Filho M, Boasquevisque EM, Alcantara Gomes R: Inativação de bactérias pelo cloreto estanoso. Congr Asoc Latinoam Soc Biol Med Nucl Mexico (abstr): 109, 1987

  30. Bernardo-Filho M, Cunha MC, Valsa JO, Caldeira-de-Araújo A, Silva FCP, Fonseca AS: Evaluation of potential genotoxicity of stannous chloride: Inactivation, filamentation and lysogenic induction of Escherichia coli. Food Chem Toxicol 32: 477-479, 1994

    Google Scholar 

  31. Mattos JCP, Dantas FJS, Bezerra RJAC, Bernardo-Filho M, Cabral-Neto JB, Leitão CLAC, Caldeira-de-Araújo A: Damage induced by stannous chloride in plasmid DNA. Toxicol Lett 116: 159-163, 2000

    Google Scholar 

  32. Reiniger IW, Silva CR, Felzenszwalb I, Mattos JPC, Oliveira JF, Dantas FJS, Bezerra AAC, Bernardo-Filho M: Boldine action against the stannous chloride effect. J Ethnopharmacol 68: 345-348, 1999

    Google Scholar 

  33. Canadian Council on Animal Care: Guide to the Care and Use of Experimental Animals, 1984

  34. Callahan RJ, Rabito CA: Radiolabeling of erythrocytes with technetium-99m: Role of band-3 protein in the transport of pertechnetate across the cell membrane. J Nucl Med 31: 2004-2008, 1990

    Google Scholar 

  35. Bernardo-Filho M, Moura INN, Boasquevisque EM: Technetium-99m-labeled red blood cells ‘in vitro'. Arq Biol Tecnol 26: 455-461, 1983

    Google Scholar 

  36. Bernardo-Filho M, Caniné MS, Lopes RLSF, Boasquevisque EM: Effect of temperature on the ‘in vitro’ labeling of red blood cells with technetium-99m. Arq Biol Tecnol 29: 407-412, 1986

    Google Scholar 

  37. Bernardo-Filho M, Pereira JAA, Boasquevisque EM, Hassón-Voloch A: Technetium-99m distribution into Klebsiella pneumoniae. J Nucl Biol Med 35: 162-166, 1991

    Google Scholar 

  38. Howard-Flanders P, Simson E, Theriot L: A locus that controls filament formation and sensitivity to radiation in E. coli K-12. Genetics 49: 237-246, 1964

    Google Scholar 

  39. Luria SE, Burrous JW: Hybridization between E. coli and Shigella. J Bacteriol 74: 461-476, 1957

    Google Scholar 

  40. Dantas FJS, Moraes MO, Carvalho EF, Valsa JO, Bernardo-Filho M, Caldeira-de-Araújo A: Lethality induced by stannous chloride on Escherichia coli AB1157: Participation of reactive oxygen species. Food Chem Toxicol 34: 959-962, 1996

    Google Scholar 

  41. Hladik WB III, Nigg KKE, Rhodes BA: Drug-induced changes in the biologic distribution of radiopharmaceuticals. Sem Nucl Med 9: 184-192, 1982

    Google Scholar 

  42. Sampson CB: Complications and difficulties in radiolabeling blood cells: A review. Nucl Med Commun 17: 648-658, 1996

    Google Scholar 

  43. Reiniger IW, Oliveira JF, Caldeira-de-Araújo A, Bernardo-Filho M: Effect of Peumus boldus on the labeling of red blood cells and plasma proteins with technetium-99m. Appl Radiat Isotopes 51: 145-149, 1999

    Google Scholar 

  44. Lima EAC, Diré G, Mattos DMM, Freitas RS, Gomes ML, Oliveira MBN, Faria MCV, Jales RL, Bernard Filho M: Effect of an extract of cauliflower (leaf) on the labeling of blood elements with technetium-99m and on the survival of Escherichia coli AB1157 submitted to the treatment with stannous chloride. Food Chem Toxicol 2002 (in press)

  45. Grases F, Ramis M, Costa-Bauzá A: Effects of phytate and pyrophosphate on brushite and hydroxyapatite crystallization. Urol Res 28: 136-140, 2000

    Google Scholar 

  46. Felzenszwalb I, De Mattos JCP, Bernardo-Filho M, Caldeira-de-Araújo A: Shark cartilage-containing preparation: Protection against reactive oxygen species. Food Chem Toxicol 36: 1079-1084, 1998

    Google Scholar 

  47. Dantas FJS, Moraes MO, De Mattos JCP, Bezerra RJAC, Carvalho EF, Bernardo-Filho M, Caldeira-de-Araújo A: Stannous chloride mediates single strand breaks in plasmid DNA through reactive oxygen species formation. Toxicol Lett 110: 129-136, 1999

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Av. 28 de setembro 87, Rio de Janeiro, RJ, 20551-030, Brazil

    G.L. Lima-Filho

  2. Departamento de Medicina Tropical, Hospital das Clínicas, Universidade Federal de Pernambuco, Recife, PE, Brazil

    G.M.T. Lima

  3. Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    R.S. Freitas, L.C.M. Aleixo, S.R.F. Moreno & M. Bernardo-Filho

  4. Departamento de Biofísica e Radiobiologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, Brazil

    M.T.J.A. Catanho

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  1. G.L. Lima-Filho
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Lima-Filho, G., Lima, G., Freitas, R. et al. Evaluation of the phytic acid effect on the labeling of blood elements with technetium-99m and on the survival of a strain of Escherichia coli treated with stannous fluoride. Mol Cell Biochem 247, 121–126 (2003). https://doi.org/10.1023/A:1024111006286

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