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Expression of neuronal plasticity markers in hypoglycemia induced brain injury

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Abstract

The expression of neuroplasticity markers was analyzed in four brain regions, namely cerebral hemispheres (CH), cerebellum (CB), brain stem (BS) and diencephalon (DC) from insulin-induced hypoglycemic young adult rats. Significant decrease in neural cell adhesion molecule (NCAM) isoforms and growth-associated protein-43 (GAP-43) was observed following hypoglycemic injury from majority of brain regions studied. The glial fibrillary acidic protein (GFAP) level increased significantly in cerebral hemispheres and diencephalon regions, whereas, synaptophysin level increased in cerebellum, brain stem and diencephalon regions. The selective downregulation of the neuronal plasticity marker proteins (GAP-43 and NCAM), and enhanced expression of GFAP and synaptophysin suggests that in acute hypoglycemia, mechanisms other than energy failure may also contribute to neuronal cell damage in the brain.

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References

  1. Agardh CD, Kalimo H, Olsson Y, Siesjo BK: Hypoglycemic brain injury: Metabolic and structural findings in rat cerebellar cortex during profound insulin-induced hypoglycemia and in the recovery period following glucose administration. J Cereb Blood Flow Metab 1: 71-84, 1981

    Google Scholar 

  2. Anderson PN, Campbell G, Zhang Y, Lieberman AR: Cellular and molecular correlates of the regeneration of adult mammalian CNS axons into peripheral nerve grafts. Prog Brain Res 117: 211-233, 1998

    Google Scholar 

  3. Auer RN, Wieloch T, Olsson Y, Siesjo BK: The distribution of hypoglycemic brain damage. Acta Neuropathologica (Berlin) 64: 177-191, 1984

    Google Scholar 

  4. Babb TL, Kupfer WR, Pretorius J, Crandall PH, Levesque MF: Synaptic reorganization by mossy fibers in human epileptic fascia dentata. Neuroscience 42: 351-363, 1991

    Google Scholar 

  5. Bakken IJ, White LR, Unsgard G, Aasly J, Sonnewald U: [U-13C] glutamate metabolism in astrocytes during hypoglycemia and hypoxia. J Neurosci Res 51: 636-645, 1998

    Google Scholar 

  6. Bellier JP, Sacchettoni S, Prodhon C, Perret-Liaudet A, Belin MF, Jacquemont B: Glutamic acid decarboxylase-expressing astrocytes exhibit enhanced energetic metabolism and increase PC12 cell survival under glucose deprivation. J Neurochem 75: 56-64, 2000

    Google Scholar 

  7. Bendotti C, Baldessari S, Pende M, Southgate T, Guglielmetti F, Samanin R: Relationship between GAP-43 expression in the dentate gyrus and synaptic reorganization of hippocampal mossy fibers in rats treated with kainic acid. Eur J Neurosci 9: 93-101, 1997

    Google Scholar 

  8. Benowitz LI, Perrone-Bizzozero NI: The expression of GAP-43 in relation to neuronal growth and plasticity: when, where, how and why? In: W.H. Gispen, A. Routtenberg (eds). Protein Kinase C and its Brain Substrates. Progress in brain Research, vol. 89. Elsevier, Amsterdam, 1991, pp 69-87

    Google Scholar 

  9. Benowitz LI, Routtenberg A: GAP-43: An intrinsic determinant of neuronal development and plasticity. Trends Neurosci 20: 84-91, 1997

    Google Scholar 

  10. Callahan LM, Selski DJ, Martzen MR, Cheetham JE, Coleman PD: Preliminary evidence: Decreased GAP-43 message in tangle-bearing neurons relative to adjacent tangle-free neurons in Alzheimer's disease parahippocampal gyrus. Neurobiol Aging 15: 381-386, 1994

    Google Scholar 

  11. Canady KS, Rubel EW: Rapid and reversible astrocytic reaction to afferent activity blockade in chick cochlear nucleus. J Neurosci 12: 1001-1009, 1992

    Google Scholar 

  12. Casoli T, Spagna C, Fattoretti P, Gesuita R, Bertoni-Freddarri C: Neuronal plasticity in aging: A quantitative immunohistochemical study of GAP-43 distribution in discrete regions of the rat brain. Brain Res 714: 111-117, 1996

    Google Scholar 

  13. Cotman CW, Hailer NP, Pfister KK, Soltesz I, Schachner M: Cell adhesion molecules in neural plasticity and pathology: Similar mechanisms, distinct organizations? Prog Neurobiol 55: 659-669, 1998

    Google Scholar 

  14. Eastwood S L, Burnet PWJ, Harrison PJ: Altered synaptophysin expression as a marker of synaptic pathology in schizophrenia. Neuroscience 66: 309-319, 1995

    Google Scholar 

  15. Jorgensen OS, Riederer P: Increased synaptic markers in hippocampus of depressed patients. J Neural Trans 64: 55-66, 1985

    Google Scholar 

  16. Kaur G, Arora SK: Acetylcholinesterase and Na+, K+-ATPase activities in different regions of rat brain during insulin-induced hypoglycemia. Mol Chem Neuropathol 21: 83-93, 1994

    Google Scholar 

  17. Kaur G, Singh R, Baquer NZ: Changes in hexokinase isoenzymes in regions of rat brain during insulin-induced hypoglycemia. J Neurochem 41: 594-596, 1983

    Google Scholar 

  18. Kobayashi NR, Fan DP, Giehl KM, Bedard AM, Wiegand SJ, Tetzlaff W: BDNF and NT-4/5 prevent atrophy of rat rubrospinal neurons after cervical axotomy, stimulate GAP-43 and Tα1-Tubulin mRNA expression, and promote axonal regeneration. J Neurosci 17: 9583-9598, 1999

    Google Scholar 

  19. Lipoldt A, Andbjer B, Rosen L, Richter E, Ganten D, Cro Y, Petterson RF, Fuxe K: Photochemically induced focal cerebral ischemia in rat: Time dependent and global increase in expression of basic fibroblast growth factor mRNA. Brain Res 824: 153-160, 1993

    Google Scholar 

  20. Lyons SA, Kettenmann H: Oligodendrocytes and microglia are selectively vulnerable to combined hypoxia and hypoglycemia injury in vitro. J Cereb Blood Flow metab 18: 521-530, 1998

    Google Scholar 

  21. Marios CP, Iphigenia LK, Laura LD, Rona GG: Vulnerability to glucose deprivation injury correlates with glutathione levels in astrocytes. Brain Res 748: 151-156, 1997

    Google Scholar 

  22. Michelle LM, Justin C-B, Scott EH, Kathleen MR-S: Free radicals are involved in the damage to protein synthesis after anoxia/aglycemia and NMDA exposure. Brain Research 857: 172-182, 2000

    Google Scholar 

  23. Mohseni S: Hypoglycemic neuropathy. Acta Neuropathol (Berl) 102: 413-421, 2001

    Google Scholar 

  24. Okeda R, Shibutani M, Matsuo T, Tajima T: Pathological effects of maternal hypoglycemia on fetal development in cats. Acta Pathol Jpn 42: 316-324, 1992

    Google Scholar 

  25. Ono K, Asou H, Yamada M, Tokunaga A: Gradient expression of neural cell adhesion molecule (NCAM) in the pontine migratory stream of fetal cells. Neurosci Res 15: 221-223, 1992

    Google Scholar 

  26. Reier PJ: Gliosis following injury: The anatomy of astrocyte scar and their influences on axonal elongation in astrocytes. Cell Biol Pathol 3: 263-264, 1986

    Google Scholar 

  27. Sandi C, Loscertales M: Opposite effects on NCAM expression in the rat frontal cortex induced by acute vs. chronic corticosterone treatments. Brain Res 828: 127-134, 1999

    Google Scholar 

  28. Sandi C, Merino JJ, Cordero MI, Touyarot K, Venero C: Effects of chronic stress on contextual fear conditioning and the hippocampal expression of the neural cell adhesion molecule, its polysialylation and L1. Neuroscience 102: 329-339, 2001

    Google Scholar 

  29. Shimada A, Morita T, Ikeda N, Torii S, Haruna A: Hypoglycemic brain lesions in a dog with insulinoma. J Comp Pathol 122: 67-71, 2000

    Google Scholar 

  30. Skene JHP: Axonal growth associated proteins. Annu Rev Neurosci 12: 127-156, 1989

    Google Scholar 

  31. Westenbroch RE, Bausch SB, Lin RCS, Franck JE, Noebels JL, Catterall WA: Upregulation of L-type Ca2+ channels in reactive astrocytes after brain injury, hypomyelination and ischemia. J Neurosci 18: 2321-2334, 1998

    Google Scholar 

  32. Yazaki T, Asou H, Arimoto K, Toya S, Uyemura K: Decrease of NCAM expression and astrocyte-neuron interaction in long-term cultured astrocytes. Neuroreport 6: 1085-1088, 1995

    Google Scholar 

  33. Yi Li MD, Ning Jiang MD, Cecylia Powers BS, Michael Chopp PhD: Neuronal damage and plasticity identified by microtubule associated protein 2, growth-associated protein 43, and cyclin D1 immunore-activity after focal cerebral ischemia in rats. Stroke 29: 1972-1981, 1998

    Google Scholar 

  34. Zimmer LA, Ennis M, Shipley MT: Soman-induced seizures rapidly activate astrocytes and microglia in discrete brain regions. J Comp Neurol 378: 482-492, 1997

    Google Scholar 

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Authors and Affiliations

  1. Neurochemistry and Neuroendocrinology Laboratory, Department of Biotechnology, Guru Nank Dev University, Amritsar, 143005 (PB), India

    Pawan Singh, Pawan Kumar Heera & Gurcharan Kaur

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  2. Pawan Kumar Heera
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  3. Gurcharan Kaur
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Singh, P., Kumar Heera, P. & Kaur, G. Expression of neuronal plasticity markers in hypoglycemia induced brain injury. Mol Cell Biochem 247, 69–74 (2003). https://doi.org/10.1023/A:1024105120087

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