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Functional identification of rub52 gene involved in the biosynthesis of rubradirin

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Abstract

An open reading frame, rub52, has been identified as a gene encoding thymidine diphospho-glucose 2,3-dehydratase by sequence analysis of the rubradirin biosynthetic gene cluster of Streptomyces achromogenes var. rubradiris NRRL3061.The gene codes for a protein consisting of 458 amino acids with calculated molecular mass of 50 862 Da. The gene was amplified and heterologously expressed in Escherichia coli as a soluble His-tagged fusion protein. C-2 deoxygenation functionality of thymidine diphospho-4-keto-6-deoxyglucose was assigned to the rub52 gene product from in vitro enzyme assay.

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Authors and Affiliations

  1. Institute of Biomolecule Reconstruction, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam, 336-708, South Korea

    Jyoti Maharjan, Kwangkyoung Liou, Hei Chan Lee & Jae Kyung Sohng

  2. School of Advanced Materials and Process Engineering, Inje University, Kimhae , 621-749, Korea

    Chun-Gyu Kim

  3. Korea Research Institute of Bioscience and Biotechnology, Taejon , 305-600, Korea

    Jung-Joon Lee

  4. Department of Pharmacy, Chosun University, Kwangju , 501-759, Korea

    Jin-Cheol Yoo

Authors
  1. Jyoti Maharjan
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  2. Kwangkyoung Liou
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  3. Hei Chan Lee
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  4. Chun-Gyu Kim
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  5. Jung-Joon Lee
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  6. Jin-Cheol Yoo
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  7. Jae Kyung Sohng
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Corresponding author

Correspondence to Jae Kyung Sohng.

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Maharjan, J., Liou, K., Lee, H.C. et al. Functional identification of rub52 gene involved in the biosynthesis of rubradirin. Biotechnology Letters 25, 909–915 (2003). https://doi.org/10.1023/A:1024075902143

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  • DOI: https://doi.org/10.1023/A:1024075902143

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