Abstract
A new method for growing isolated wheat zygotes by co-cultivation with microspores isolated from the same species has been developed. Although the mortality of isolated zygotes within 6 h after the transfer into the nurse culture was relatively high (70%) in this method, the majority of the structures which survived developed into fertile plants (61%). Zygotic structures grown in these cultures were morphologically not as regular as embryos grown in planta, but they resembled them closely in cytological properties and developmental pattern.
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Bakos, F., Darkó, É., Pónya, Z. et al. Regeneration of fertile wheat (Triticum aestivum) plants from isolated zygotes using wheat microspore culture as nurse cells. Plant Cell, Tissue and Organ Culture 74, 243–247 (2003). https://doi.org/10.1023/A:1024071121083
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DOI: https://doi.org/10.1023/A:1024071121083