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A peptide antibody for rapid screening of Streptomyces species producing phospholipase D

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Abstract

By examining the conserved regions in the protein sequences of eight different Streptomyces phospholipase Ds (PLD) reported so far and the X-ray crystallographic structure of a Streptomyces PLD, we designed a peptide sequence, DPANRGAVGSGGYSQIKSL, for the screening of microorganisms producing PLD. In the enzyme-linked immunosorbent assay using a mouse antibody raised against the designed peptide, we recovered seven producing strains out of 128 soil isolates.

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Authors and Affiliations

  1. Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, 561-756, Republic of Korea

    Tai-Boong Uhm & Sun Hee Lee

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  1. Tai-Boong Uhm
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  2. Sun Hee Lee
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Correspondence to Tai-Boong Uhm.

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Uhm, TB., Lee, S.H. A peptide antibody for rapid screening of Streptomyces species producing phospholipase D. Biotechnology Letters 25, 883–886 (2003). https://doi.org/10.1023/A:1024061028164

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  • DOI: https://doi.org/10.1023/A:1024061028164

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