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Impacts of silviculture on genetic diversity in the native forest species Eucalyptus sieberi

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Abstract

Potential impacts of regeneration practices ongenetic diversity in the Australian nativeforest species Eucalyptus sieberi L.A.S.Johnson. (silvertop ash) were assessed usingDNA markers. Three different silviculturaltreatments were examined: clear-felling withaerial re-sowing, and the seed tree system withsite preparation by either burning ormechanical disturbance. In addition, twounharvested stands were chosen as controls. Atotal sample of 825 trees were genotyped at 35Mendelian markers: 26 single-copy nuclear RFLPsand 9 microsatellites. No significantdifferences were found among the treatments inany of four population genetic statistics:allelic richness, effective number of alleles,expected heterozygosity and the panmictic index(f). Rare alleles were prevalent, and a MonteCarlo simulation showed that the apparent lossof four rare alleles from the saplingregenerants was highly statisticallysignificant. There was no evidence for recentbottlenecks from analyses of either the levelsof expected heterozygosity relative to thatexpected under mutation drift equilibrium, orthe allele frequency profiles. A dendrogram ofthe relationships between the sampledpopulations suggested that the seed tree systemmay result in the promotion of genetic drift(slight expansion of the dendrogram) whileaerial re-sowing of clear falls with the sameseedlot will lead to genetic homogenisation(contraction of the dendrogram). The apparentgenetic robustness of E. sieberi tonative forest regeneration practices isattributed to its local abundance combined withthe favourable properties of its reproductivebiology, as well as to the limitation that onlya single rotation was examined.

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References

  • Adams WT, Zuo J, Shimizu JY, Tappeiner JC (1998) Impact of alternative regeneration methods on genetic diversity in coastal Douglas-fir. For. Sci., 44, 390-396.

    Google Scholar 

  • Bassett OD (2001) Seed supply 1: Development of seed crops in Eucalyptus sieberi and E. globoidea in a lowland sclerophyll forest in East Gippsland, Victoria. Australian Forestry, in press.

  • Boland DJ, Brooker MIH, Chippendale GM, Hall N, Hyland BPM, Johnston RD, Kleinig DA, Turner JD (1984) Forest Trees of Australia. Nelson Wadsworth and CSIRO, Melbourne.

    Google Scholar 

  • Booy G, Hendriks RJJ, Smulders MJM, van Groenendael JM, Vosman B (2000) Genetic diversity and the survival of populations. Plant Biology, 2, 379-395.

    Google Scholar 

  • Buchert GP, Rajora OP, Hood JV, Dancik BP (1997) Effects of harvesting on genetic diversity in old-growth eastern white pine in Ontario, Canada. Conserv. Biol., 11, 747-758.

    Google Scholar 

  • Byrne M, Moran GF, Tibbits WN (1993) Restriction map and maternal inheritance of chloroplast DNA in Eucalyptus nitens. J. Hered. 84, 218-220.

    Google Scholar 

  • Byrne M, Moran GF, Murrell JC, Tibbits WN (1994) Detection and inheritance of RFLPs in Eucalyptus nitens. Theor. Appl. Genet., 89, 397-402.

    Google Scholar 

  • Byrne M, Murrell JC, Allen B, Moran GF (1995) An integrated genetic linkage map for eucalypts using RFLP, RAPD and isozyme markers. Theor. Appl. Genet., 91, 869-875.

    Google Scholar 

  • Byrne M, Marquez-Garcia MI, Uren T, Smith DS, Moran GF (1996) Conservation and genetic diversity of microsatellite loci in the genus Eucalyptus. Aust. J. Bot., 44, 331-341.

    Google Scholar 

  • Byrne M, Parrish TL, Moran GF (1998) Nuclear RFLP diversity in Eucalyptus nitens. Heredity, 81, 225-233.

    Google Scholar 

  • Commonwealth of Australia (1992) National Forest Policy Statement: a New Focus for Australia's Forests. Commonwealth of Australia, Canberra.

    Google Scholar 

  • Cornuet JM, Luikart G (1996) Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics, 144, 2001-2014.

    Google Scholar 

  • Cumming F, Black P (1993) Cabbage Tree Pre-harvest Assessment. VSP Internal Report No. 21. Department Conservation and Natural Resources, Victoria, Australia.

    Google Scholar 

  • El-Kassaby YA (2000) Impacts of industrial forestry on genetic diversity of temperate forest trees. In: Forest Genetics and Sustainability (ei. Mátyás C), pp. 155-169. Kluwer Academic Publishers, the Netherlands.

    Google Scholar 

  • Featherston GR (1985) Harvesting History of a Coastal Forest Area in East Gippsland in Relation to Eucalypt Species Composition. Research Branch Report No. 290, State Forests and Lands Service, Department of Conservation, Forests and Lands, Victoria, Australia.

    Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from gene frequencies and quantitative characters: Finding maximum likelihood estimates. Evolution, 35, 1229-1242.

    Google Scholar 

  • Felsenstein J (1993) PHYLIP (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle.

  • Florence RG (1996) Ecology and Silviculture of Eucalypt Forests. CSIRO Publishing, Melbourne.

    Google Scholar 

  • Gillies ACM, Navarro C, Lowe AJ, Newton AC, Hernández M, Wilson J, Cornelius JP (1999) Genetic diversity in Mesoamerican populations of mahogany (Swietenia macrophylla), assessed using RAPDs. Heredity, 83, 722-732.

    Google Scholar 

  • Glaubitz JC, Emebiri LC, Moran GF (2001) Dinucleotide microsatellites from Eucalyptus sieberi: Inheritance, diversity and improved scoring of single-base differences. Genome, 44, 1041-1045.

    Google Scholar 

  • Goldstein DB, Pollock DD (1997) Launching microsatellites: A review of mutation processes and methods of phylogenetic inference. J. Hered., 88, 335-342.

    Google Scholar 

  • Gömöry D (1992) Effect of stand origin on the genetic diversity of Norway spruce (Picea abies Karst.) populations. Forest Ecol. Manage., 54, 215-223.

    Google Scholar 

  • Gregorius HR (1980) The probability of losing an allele when diploid genotypes are sampled. Biometry, 36, 643-652.

    Google Scholar 

  • Kimura M, Crow J (1964) The number of alleles that can be maintained in a finite population. Genetics, 49, 725-738.

    Google Scholar 

  • Knowles P (1985) Comparison of isozyme variation among natural stands and plantations: Jack pine and black spruce. Can. J. For. Res., 15, 902-908.

    Google Scholar 

  • Ledig FT, Smith DM (1981) The influence of silvicultural practices on genetic improvement: Height growth and weevil resistance in eastern white pine. Silvae Genetica, 30, 30-36.

    Google Scholar 

  • Lewis PO, Zaykin D (2000) Genetic Data Analysis: Computer program for the analysis of allelic data. Version 1.0 (d15). Free program distributed by the authors over the internet from the GDA Home Page at http://lewis.eeb.uconn.edu/lewishome/gda. html.

  • Luikart G, Allendorf FW, Cornuet J-M, Sherwin WB (1998a) Distortion of allele frequency distributions provides a test for recent population bottlenecks. J. Hered., 89, 238-247.

    Google Scholar 

  • Luikart G, Sherwin WB, Steele BM, Allendorf FW(1998b) Usefulness of molecular markers for detecting population bottlenecks via monitoring genetic change. Mol. Ecol., 7, 963-974.

    Google Scholar 

  • Lutze M (1998) Species Composition in Mixed Species Regeneration Following a Range of Harvesting and Site Preparation Treatments in a Lowland Eucalypt Forest in East Gippsland. VSP Technical Report No. 33, Department of Natural Resources and Environment, Victoria, Australia.

    Google Scholar 

  • Manly BFJ (1997) Randomization, Bootstrap and Monte Carlo Methods in Biology. Chapman &; Hall, London.

    Google Scholar 

  • National Forest Inventory (1997) National Plantation Inventory of Australia. Bureau of Resource Sciences, Canberra.

    Google Scholar 

  • Neale DB (1985) Genetic implications of shelterwood regeneration of Douglas-fir in Southwest Oregon. Forest Sci., 31, 995-1005.

    Google Scholar 

  • Newman D, Pilson D (1997) Increased probability of extinction due to decreased genetic effective population size: Experimental populations of Clarkia pulchella. Evolution, 51, 354-362.

    Google Scholar 

  • Perry DJ, Bousquet J (2001) Genetic diversity and mating system of post-fire and post-harvest black spruce: An investigation using codominant sequence-tagged-site (STS) markers. Can. J. For. Res., 31, 32-40.

    Google Scholar 

  • Piry S, Luikart G, Cornuet J-M (1999) BOTTLENECK:A computer program for detecting recent reductions in the effective population size using allele frequency data. J. Hered., 90, 502-503.

    Google Scholar 

  • Rajora OP (1999) Genetic biodiversity impacts of silvicultural practices and phenotypic selection in white spruce. Theor. Appl. Genet., 99, 954-961.

    Google Scholar 

  • Rajora OP, Rahman MH, Buchert GP, Dancik BP (2000) Microsatellite analysis of genetic effects of harvesting in old-growth eastern white pine (Pinus strobus) in Ontario, Canada. Mol. Ecol., 9, 339-348.

    Google Scholar 

  • Ratnam W, Lee CT, Muhammad N, Boyle TJB (2000) Impact of logging on genetic diversity in humid tropical forests. In: Forest Genetics and Sustainability (ed. Mátyás C), pp. 171-181. Kluwer Academic Publishers, the Netherlands.

    Google Scholar 

  • Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res., 13, 7207-7221.

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  • SAS Institute Inc. (1989) SAS/STAT ê User's Guide, Version 6, Fourth Edition, Volume 2. Cary, North Carolina, USA.

    Google Scholar 

  • Sjögren P, Wyöni P-I (1994) Conservation genetics and detection of rare alleles in finite populations. Conserv. Biol., 8, 267-270.

    Google Scholar 

  • Stoehr MU, El-Kassaby YA (1997) Levels of genetic diversity at different stages of the domestication cycle of interior spruce in British Columbia. Theor. Appl. Genet., 94, 83-90.

    Google Scholar 

  • Stuwe J, Mueck SG (1990) Vegetation Survey and Classification of the Cabbage Tree Creek Study Area. SSP Technical Report No. 2. Lands and Forests Division, Department of Conservation, Forests and Lands, Victoria, Australia.

    Google Scholar 

  • Thamarus KA, Groom K, Murrell J, Byrne M, Moran GF (2001) A genetic linkage map for Eucalyptus globulus with candidate loci for wood, fibre and floral traits. Theor. Appl. Genet., 104, 379-387.

    Google Scholar 

  • Thomas BR, Macdonald SE, Hicks M, Adams DL, Hodgetts, RB (1999) Effects of reforestation methods on genetic diversity of lodgepole pine: An assessment using microsatellite markers and randomly amplified polymorphic DNA markers. Theor. Appl. Genet., 98, 793-801.

    Google Scholar 

  • Weir BS (1996) Genetic Data Analysis II: Methods for Discrete Population Genetic Data. Sinauer Associates, Sunderland, Massachusetts.

    Google Scholar 

  • Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution, 38, 1358-1370.

    Google Scholar 

  • Westemeier RL, Brawn JD, Simpson SA, Esker TL, Jansen RW, Walk JW, Kershner EL, Bouzat JL, Paige KN (1998) Tracking the long-term decline and recovery of an isolated population. Science, 282, 1695-1698.

    Google Scholar 

  • Yeh FC, Yang R-C, Boyle TBJ, Ye Z-H, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada. http://www.ualberta.ca/∼fyeh/index.htm.

    Google Scholar 

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Authors and Affiliations

  1. CSIRO Forestry & Forest Products, P.O. Box E4008, Kingston ACT, 2604, Australia

    Jeffrey C. Glaubitz, Harry X. Wu & Gavin F. Moran

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  1. Jeffrey C. Glaubitz
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  2. Harry X. Wu
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  3. Gavin F. Moran
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Correspondence to Jeffrey C. Glaubitz.

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Glaubitz, J.C., Wu, H.X. & Moran, G.F. Impacts of silviculture on genetic diversity in the native forest species Eucalyptus sieberi . Conservation Genetics 4, 275–287 (2003). https://doi.org/10.1023/A:1024025331750

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