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Stability of tumor necrosis factor-α during ion exchange chromatography

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Abstract

Denaturation of target protein by various separation and purification steps contributes significant part to the total product loss in bioseparation. The conformational change and accompanying loss of activity of tumor necrosis factor-α during ion exchange chromatography was reversible and was decreased by adding polyethylene glycol 200 at 2 to 5% (v/v) to the eluting solution.

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.R. China

    Ming Li, Yunshan Wang, Weijun Li & Zhiguo Su

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  1. Ming Li
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  2. Yunshan Wang
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  3. Weijun Li
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  4. Zhiguo Su
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Correspondence to Zhiguo Su.

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Li, M., Wang, Y., Li, W. et al. Stability of tumor necrosis factor-α during ion exchange chromatography. Biotechnology Letters 25, 905–908 (2003). https://doi.org/10.1023/A:1024019817164

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  • DOI: https://doi.org/10.1023/A:1024019817164

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