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Application of statistically-based experimental designs for the optimization of nisin production from whey

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Abstract

Statistically-based experimental designs were applied for the optimization of nisin production by Lactococcus lactis in a whey-based medium. Yeast extract, KH2PO4, and MgSO4 were identified to have significant effects on nisin biosynthesis by a Plackett–Burman design. These three significant factors were subsequently optimized using central composite design, and the optimal conditions were determined to be 12.067 g l−1 for yeast extract, 0.569 g l−1 for KH2PO4, and 0.572 g l−1 for MgSO4. The validity of the optimal conditions was verified by a separate experiment.

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Authors and Affiliations

  1. Department of Biological Systems Engineering, Washington State University, Pullman, WA, 99164-6120, USA

    Chuanbin Liu, Yan Liu, Wei Liao, Zhiyou Wen & Shulin Chen

Authors
  1. Chuanbin Liu
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  2. Yan Liu
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  3. Wei Liao
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  4. Zhiyou Wen
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  5. Shulin Chen
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Correspondence to Shulin Chen.

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Liu, C., Liu, Y., Liao, W. et al. Application of statistically-based experimental designs for the optimization of nisin production from whey. Biotechnology Letters 25, 877–882 (2003). https://doi.org/10.1023/A:1024009027255

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  • DOI: https://doi.org/10.1023/A:1024009027255

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