Skip to main content
SpringerLink
Log in

Specific binding of ribosome recycling factor (RRF) with the Escherichia coli ribosomes by BIACORE

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

The direct assays on Biacore with immobilised RRF and purified L11 from E. coli in the flow trough have shown unspecific binding between the both proteins. The interaction of RRF with GTPase domain of E. coli ribosomes, a functionally active complex of L11 with 23S r RNA and L10.(L7/L12)4 was studied by Biacore. In the experiments of binding of RRF with 30S, 50S and 70S ribosomes from E. coli were used the antibiotics thiostrepton, tetracycline and neomycin and factors, influencing the 70S dissociation Mg2+, NH4Cl, EDTA. The binding is strongly dependent from the concentrations of RRF, Mg2+, NH4Cl, EDTA and is inhibited by thiostrepton. The effect is most specific for 50S subunits and indicates that the GTPase centre can be considered as a possible site of interaction of RRF with the ribosome. We can consider an electrostatic character of the interactions with most probable candidate 16S and 23S r RNA at the interface of 30S and 50S ribosomal subunits.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Janosi L, Shimizu I & Kaji A (1994) Proc. Natl. Acad. Sci. USA 91: 4249–4253.

    Google Scholar 

  2. Pavlov MY, Freistroffer DV, MacDougall J, Buckingham RH & Ehrenberg M (1997) EMBO J. 16: 4134–4141.

    Google Scholar 

  3. Kaji A, Teyssier E & Hirokawa G (1998) Biochem. Biophys. Res. Commun. 250 (1): 1–4 (review).

    Google Scholar 

  4. Inokuchi Y, Hirashima A, Seikine Y, Janosi L & Kaji A (2000) EMBO J. 19: 3788–3798.

    Google Scholar 

  5. Janosi L, Ricker R & Kaji A (1996) Biochimie 78: 959–969.

    Google Scholar 

  6. Janosi L, Mori H, Sekine Y, Abragan J, Janosi R, Hirokawa Go & Kaji A (2000) J. Mol. Biol. 295: 815–829.

    Google Scholar 

  7. Kanai T, Takeshita S, Atomi H, Umemura K, Ueda M & Tanaka A (1998) Eur. J. Biochem. 256 (1): 212–220.

    Google Scholar 

  8. Rolland N, Janosi L, Block MA, Shuda M, Teyssier E, Miege C, Cheniclet C, Carde JP, Kaji A & Joyard J (1999) Proc. Natl. Acad. Sci. USA 96 (10): 5464–5469.

    Google Scholar 

  9. Selmer M, Al-Karadaghi S, Hirokawa G., Kaji A & Liljas A (1999) Science 286 (5448): 2349–2352.

    Google Scholar 

  10. Toyoda T, Tin OF, Ito K, Fujiwara T, Kumasaka T, Yamamoto M, Garber MB & Nakamura Y (2000) RNA 6 (10): 1432–1444.

    Google Scholar 

  11. Kim KK, Min K & Suh SW ( 2000) EMBO J. 19: 2362–2370.

    Google Scholar 

  12. Yoshida T, Uchiyama S, Nakano H, Kashimori H, Kajima H, Oshima T, Saihara Y, Ishino T, Shimahara H, Yoshida T, Yokose K, Ohkubo T, Kaji A & Kobayashi Y (2001) Biochemistry, 40: 2387–2396.

    Google Scholar 

  13. Jelens PC (1980) Anal. Biochem. 105: 369–374.

    Google Scholar 

  14. Karlsson M, Pavlov MP, Malmqvist M, Persson B & Ehrenberg M (1999) Biochimie 81: 995–1002.

    Google Scholar 

  15. Hirashima A & Kaji A (1973) J. Biol. Chem. 248: 7580–7587.

    Google Scholar 

  16. Xing, Y. & Draper, D.E. (1995) J. Mol. Biol. 249: 319–331.

    Google Scholar 

  17. Blyn LB, Risen LM, Griffey RH & Draper DE (2000) 28 (8): 1778–1784.

  18. Ishino T, Atarashi K, Uchiyama S, Yamami T, Saihara Y, Yoshida T, Hara H, Yokose K, Kobayashi Y & Nakamura Y (2000) Genes to Cells 5: 953–963.

    Google Scholar 

  19. Hirokawa G., Kiel MC, Muto A, Selmer M, Raj VS, Liljas A, Igarashi K, Kaji H & Kaji A (2002) EMBO J. 21 (9): 2272–2281.

    Google Scholar 

  20. Hirokawa G, Kiel M, Muto A, Kawai G, Igarashi K, Kaji H & Kaji A (2002) J. Biol. Chem. (in press).

  21. Frank J & Agrawal RK (2000) Nature 407: 318–322.

    Google Scholar 

  22. Wimberly BT, Guyman R, McCutcheon JP, White SW & Ramakrishnan V (1999) Cell 97: 491–502.

    Google Scholar 

  23. Munishkin A & Wool IG (1995) J. Biol. Chem. 270(51): 30581–30587.

    Google Scholar 

  24. Wilson KS & Noller HF (1998) Cell 92(1): 131–139.

    Google Scholar 

  25. Bukhman YV & Draper DE (1997) J. Mol. Biol. 273(5): 1020–1031.

    Google Scholar 

  26. Wang YX, Lu M & Draper DE (1993) Biochemistry 32(46): 12279–12282.

    Google Scholar 

  27. Fujiwara T, Ito K & Nakamura Y (2001) RNA 7: 64–70.

    Google Scholar 

  28. Agrawal RK, Penczek P, Grassucci RA & Frank J (1998) Proc. Natl. Acad. Sci. USA 95: 6134–6138.

    Google Scholar 

  29. Stark H, Rodnina MV, Rinke-Appel J, Brimacombe R, Wintermeyer W & van Heel M (1997) Nature 389: 403–406.

    Google Scholar 

  30. Moazed D & Noller HF (1986) Cell 47: 985–994.

    Google Scholar 

  31. Moazed D & Noller HF (1989) Cell 57: 585–597.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bulgarian Academy of Sciences, Institute of Biophysics, 1113, Sofia, Bulgaria

    Roumiana T. Todorova

  2. Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, 565-0871, Japan E-mail:

    Yukari Saihara

Authors
  1. Roumiana T. Todorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yukari Saihara
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Todorova, R.T., Saihara, Y. Specific binding of ribosome recycling factor (RRF) with the Escherichia coli ribosomes by BIACORE. Mol Biol Rep 30, 113–119 (2003). https://doi.org/10.1023/A:1023991026045

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1023991026045

Search

Navigation