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NMR confirmation of an alkali-insoluble glucan from Kluyveromyces marxianus cultivated on a lactose-based medium

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Abstract

Yeast cells of Kluyveromyces marxianus grown in a sterile lactose-based medium, were extracted with sodium hydroxide to give a fraction of alkali-insoluble yeast (AIY) glucan. From 1H- and 13C-NMR characterization it was concluded that the glucan consisted mostly of β(1 → 3) linkages and was similar to that obtained from Saccharomyces cerevisiae used in the preparations for skin revitalization and repair. The molecular weight of the glucan was determined to be approximately 3 × 105 Da. Therefore, K. marxianus grown on a lactose-based medium similar to whey, a waste stream of the dairy industry, could be a commercial source of β(1 → 3) glucans for use in the food and pharmaceutical industries.

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Authors and Affiliations

  1. School of Civil and Environmental Engineering, The University of New South Wales, UNSW, Sydney, 2052, Australia

    Tredwell Lukondeh, Nicholas J. Ashbolt, Peter L. Rogers & James M. Hook

Authors
  1. Tredwell Lukondeh
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  2. Nicholas J. Ashbolt
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  3. Peter L. Rogers
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  4. James M. Hook
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Lukondeh, T., Ashbolt, N.J., Rogers, P.L. et al. NMR confirmation of an alkali-insoluble glucan from Kluyveromyces marxianus cultivated on a lactose-based medium. World Journal of Microbiology and Biotechnology 19, 349–355 (2003). https://doi.org/10.1023/A:1023961411821

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  • DOI: https://doi.org/10.1023/A:1023961411821