Abstract
Suspension cultures of Eucommia ulmoides were developed and shown to accumulate chlorogenic acid. MS medium plus 2.0 mg l−1 2,4-dichlorophenoxy acetic acid was used for the cell suspension cultures of Eucommia ulmoides. The chlorogenic acid content of suspension cells was analyzed by capillary electrophoresis, and the mean content was 2.15%, approximate to that of Eucommia ulmoides leaves.
Similar content being viewed by others
References
Li JS & Yan YN (1996) A preliminary analysis on leaves and barks of Eucommia ulmoides (Chinese). Bull. Chin. Trad. Med. 11: 4-5
Ma XH, Zhang KJ, Qin WQ & Wang L (1999) Identification of the structure of chlorogenic acid from the leaves of Eucommia ulmoides. In: Zhang KJ, Su YQ, Ma XH & Che QM (eds) Proceedings of the First International Symposium On Eucommia Ulmoides (pp. 42-47). China Forestry Publishing House, Beijing
Ning W (1994) Lithospermum erythrorhizon cell suspension culture and shikonin derivatives formation (Chinese). Chin. J. Biotechnol. 10: 76-80
Tang W (1994) Studies on establishment and characterization of suspension cells in Panax Ginseng C.A. Mey (Chinese). Biotechnology 4: 26-29
Wang JL, Chen PL & Zhu BC (1994) Amino acid in Eucommia ulmoides (Chinese). J. Hebei Univ. 14: 80-82
Xu SL, Zeng QZ & Pan ZX (1985) Influence of Eucommia ulmoides on non-specific immunity of organs (Chinese). Trad. Chin. Med. 16: 15-17
Xu SL, Zhou HG & Huang WG (1983) Influence of Eucommia ulmoides on non-specific immunity of organs (Chinese). Trad. Chin. Med. 14: 27-28
Yu ZL, Zhu RY & Liu XM (1992) An analysis on the nutrition components in the leaves and bark of Eucommia ulmoides (Chinese). Trad. Chin. Med. 23: 161
Zhang KJ, Wang L & Zhang FY (1996) A comparison of the contents of bioactive constituents between leaves and bark of Eucommia ulmoides (Chinese). J. Northwestern College of Forestry 11: 42-46
Zhong JJ (1995) Effects of biological factors on cell growth and anthocyanin formation by suspended culture of Perilla rutescens cells (Chinese). Chin. J. Biotechnol. 11: 153-156
Zhu LQ, Zhang LM & Gong RS (1986) Pharmacology of the leaves and bark of Eucommia ulmoides (Chinese). Trad. Chin. Med. 17: 15-17
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, J., Liao, X., Zhang, H. et al. Accumulation of chlorogenic acid in cell suspension cultures of Eucommia ulmoides . Plant Cell, Tissue and Organ Culture 74, 193–195 (2003). https://doi.org/10.1023/A:1023957129569
Issue Date:
DOI: https://doi.org/10.1023/A:1023957129569