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New methods for data analysis of isothermal titration calorimetry

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Abstract

Heat divided by ligand concentration vs. heat, similar to the Scatchard plot, was introduced to obtain the equilibrium constant (K) and the enthalpy of binding (DH) using isothermal titration calorimetry data. Values of K and DH obtained by this linear pseudo-Scatchard plot for a system with a set of independent binding sites (such as binding fluoride ions on urease and monosaccharide methyl a-D-mannopyranoside on concavalin A) were remarkably like that obtained from a normal fitting Wiseman method and other our technical methods. On applying this graphical method to study the binding of copper ion on myelin basic protein (MBP), a concave downward curve obtained was consistent with the positive cooperativity in the binding. A graphical fitting by simple method for determination of thermodynamic parameters was also introduced. This method is general, without any assumption and restriction made in previous method. This general method was applied to the product inhibition study of adenosine deaminase.

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References

  1. J. T. Edsall and H. Gutfreund, Biothermodynamics, John Wiley & Sons, New York 1984, Chap. 6.

    Google Scholar 

  2. I. WadsØ, Quart. Rev. Biophys., 3 (1970) 383.

    Article  Google Scholar 

  3. M. L. McGlashan, Chemical Thermodynamics, Academic Press, London 1979, Chaps 3–4.

    Google Scholar 

  4. N. Langerman and R. L. Biltonen, Methods Enzymol., 61 (1979) 261.

    Article  CAS  Google Scholar 

  5. K. S. Krishnan and J. F. Brandts, Methods Enzymol., 49 (1978) 1.

    Google Scholar 

  6. P. L. Privalov, FEBS Letters, 40S (1974) S140.

    Google Scholar 

  7. P. L. Privalov, Adv. Protein Chem., 33 (1979) 167.

    CAS  Google Scholar 

  8. P. L. Privalov, Adv. Protein Chem., 35 (1982) 1.

    Article  CAS  Google Scholar 

  9. A. A. Saboury and A. A. Moosavi-Movahedi, Biochem. Educ., 22 (1994) 210.

    Article  CAS  Google Scholar 

  10. E. Freire, O. L. Mayorga and M. Straume, Anal. Chem., 62 (1990) 950A.

    CAS  Google Scholar 

  11. A. A. Saboury, A. K. Bordbar and A. A. Moosavi-Movahedi, Bull. Chem. Soc. Jpn., 69 (1996) 3031.

    Article  CAS  Google Scholar 

  12. A. A. Saboury, M. U. Dahut, S. Ghobadi, J. Chamani and A. A. Moosavi-Movahedi, J. Chin. Chem. Soc., 45 (1998) 667.

    CAS  Google Scholar 

  13. A. A. Saboury, Thermochim. Acta, 320 (1988) 97.

    Article  Google Scholar 

  14. O. A. Amire, J. Masoudy, A. A. Saboury and A. A. Moosavi-Movahedi, Thermochim. Acta, 303 (1997) 219.

    Article  CAS  Google Scholar 

  15. A. A. Saboury, A. K. Bordbar and A. A. Moosavi-Movahedi, J. Chem. Thermodyn., 28 (1996) 1077.

    Article  CAS  Google Scholar 

  16. K. Nazari, A. A. Saboury and A. A. Moosavi-Movahedi, Thermochim. Acta, 302 (1997) 131.

    Article  CAS  Google Scholar 

  17. A. K. Bordbar, A. A. Moosavi-Movahedi and A. A. Saboury, Thermochim. Acta, 287 (1996) 343.

    Article  CAS  Google Scholar 

  18. A. A. Saboury and A. A. Moosavi-Movahedi, J. Enzyme Inhibition, 12 (1997) 273.

    CAS  Google Scholar 

  19. M. Ghadermarzi, A. A. Saboury and A. A. Moosavi-Movahedi, Polish J. Chem., 72 (1998) 2024.

    CAS  Google Scholar 

  20. S. Gumpen, P. O. Hegg and H. Martens, Biochem. Biophys. Acta, 574 (1979) 189.

    CAS  Google Scholar 

  21. M. Angberg, C. Nystrom and S. Castensson, Acta Pharm. Sued., 25 (1988) 307.

    CAS  Google Scholar 

  22. L. D. Hansen, E. A. Lewis, D. J. Eatough, R. G. Bergstrom and D. DeGraft-Johnson, Pharm. Res., 6 (1989) 20.

    Article  CAS  Google Scholar 

  23. M. J. Koenigbaure, S. H. Brooks, G. Rullo and R. A. Couch, Pharm. Res., 9 (1992) 939.

    Article  Google Scholar 

  24. T. F. Bolles and R. S. Drago, J. Am. Chem. Soc., 87 (1965) 5015.

    Article  CAS  Google Scholar 

  25. M. A. Laandav, M. N. Markovich and L. A. Pyruzyan, Biochim. Biophys. Acta, 493 (1977) 1.

    Google Scholar 

  26. A. Chen and I. WadsØ, Biochem. Biophys. Meth., 6 (1982) 307.

    Article  CAS  Google Scholar 

  27. T. Wiseman, S. Williston, J. F. Brandts and L. Lin, Anal. Biochem., 179 (1989) 131.

    Article  CAS  Google Scholar 

  28. P. R. Connelly, R. Varadarajan, J. M. Sturtevant and F. M. Richards, Biochemistry, 29 (1990) 6108.

    Article  CAS  Google Scholar 

  29. J. E. Wilson and A. Chin, Anal. Biochem., 193 (1991) 16.

    Article  CAS  Google Scholar 

  30. F. Zhang and E. S. Rowe, Biochemistry, 31 (1992) 2005.

    Article  CAS  Google Scholar 

  31. D. Hamada, S. Kidokoro, H. Fukada, K. Takahashi and Y. Goto, Proc. Natl. Acad. Sci. USA, 91 (1994) 10325.

    Article  CAS  Google Scholar 

  32. R. Ramkumar, A. Surolia and S. K. Podder, Biochem. J., 308 (1995) 237.

    CAS  Google Scholar 

  33. C. S. Raman, M. J. Allan and B. Nall, Biochemistry, 34 (1995) 5831.

    Article  CAS  Google Scholar 

  34. M. C. Chervenak and E. J. Toone, Biochemistry, 34 (1995) 5685.

    Article  CAS  Google Scholar 

  35. G. Scatchard, Ann. New York Acad. Sci., 50 (1949) 660.

    Google Scholar 

  36. A. K. Bordbar, A. A. Saboury and A. A. Moosavi-Movahedi, Biochem Educ., 24 (1996) 172.

    Article  CAS  Google Scholar 

  37. A. A. Saboury, Indian J. Biochem. Biophys., 37 (2000) 347.

    CAS  Google Scholar 

  38. A. A. Saboury, Jour. Chem. Soc. Pak., 22 (2000) 204.

    Google Scholar 

  39. A. V. Hill, J. Physiol., 40 (1910) iV-Vii.

    Google Scholar 

  40. M. L. James, G. M. Smith and J. C. Wolford, Applied Numerical Methods for Digital Computer, 3rd Ed., Harper and Row Publisher, New York 1985.

    Google Scholar 

  41. W. C. Galley, M. Bouvier, S.-D. Clas, G. R. Brown and L. E. St-Pierre, Biopolymers, 27 (1988) 79.

    Article  CAS  Google Scholar 

  42. A. A. Saboury, N. Sarri-Sarraf and S. Saeidian, Thermochim. Acta, 381 (2002) 147.

    Article  CAS  Google Scholar 

  43. A. A. Saboury, A. Divsalar, G. Ataie, A. A. Moosavi-Movahedi, M. R. Housaindokht and G. H. Hakimelahi, J. Biochem. Mol. Biol., 35 (2002) 302.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, University of Tehran Tehran, Iran

    A. A. Saboury

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  1. A. A. Saboury
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Correspondence to A. A. Saboury.

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Saboury, A.A. New methods for data analysis of isothermal titration calorimetry. Journal of Thermal Analysis and Calorimetry 72, 93–103 (2003). https://doi.org/10.1023/A:1023955300221

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