Abstract
The enzymatic studies were performed to reveal a mode of activation of human topoisomerase I by a direct interaction with protein kinase CK2. In the absence of ATP CK2 kinase activated DNA relaxation about twofold. CK2α subunit was identified as solely responsible for the stimulation of relaxing activity by CK2 kinase. CK2 activated the relaxation only at the excess of the substrate over topoisomerase I. At the equimolar ratio of the substrate DNA and topoisomerase I the activation was not observed. There was also no effect of CK2 on camptothecin-induced cleavage of DNA by htopo I. These results identify an accelerated movement of topoisomerase I between substrate molecules as a cause of the activation of DNA relaxation by CK2 kinase.
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Kowalska-Loth, B., Girstun, A., Derlacz, R. et al. Activation of human topoisomerase I by protein kinase CK2. Mol Biol Rep 30, 107–111 (2003). https://doi.org/10.1023/A:1023942226954
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DOI: https://doi.org/10.1023/A:1023942226954