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Solid Phase Synthesis and Chromatographic Characteristics of Nucleophilic Agents Conjugated with Oligonucleotides Containing 5'-Terminal Carboxyl Group

Abstract

Conjugates of amines or short peptides with oligonucleotides containing 5'-terminal carboxyl group were prepared by solid phase chemical synthesis. A correlation between the physicochemical parameters and retention times of the synthesized conjugates was established by ion-pair reversed-phase HPLC.

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Correspondence to T. S. Oretskaya.

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Kachalova, A.V., Tashlitsky, V.N., Stetsenko, D.A. et al. Solid Phase Synthesis and Chromatographic Characteristics of Nucleophilic Agents Conjugated with Oligonucleotides Containing 5'-Terminal Carboxyl Group. Russian Journal of Bioorganic Chemistry 29, 274–280 (2003). https://doi.org/10.1023/A:1023940617498

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  • DOI: https://doi.org/10.1023/A:1023940617498

  • ion-pair reversed-phase HPLC
  • modified oligodeoxyribonucleotides
  • oligonucleotidopeptides