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Somatic embryogenesis and organogenesis of Agave victoriae-reginae: Considerations for its conservation

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Abstract

Agave victoriae-reginae somatic embryos were produced through a callus phase from seedling stem segments cultured on MS medium. The optimal treatment was MS medium with 2.26 μM 2,4-D. Multiple shoot regeneration was induced from axillary buds from stem segments cultured on MS medium with 2.2–4.4 μM BA. Effect of MS and modified MS medium with 50% macronutrient concentration, both containing 2.2 μM BA and sucrose at the following concentrations, 20, 30, 45 and 60 g l−1, resulted in inconsistent multiple shoot formation. Shoots and somatic embryos formed by this indirect pathway could have a multicellular origin, which might lead to genetic variation. The direct development of pre-existent buds occurred on MS basal medium and increased in the presence of BA; this might be a pathway for the rescue of genotypes of endangered species. Embryos and shoots developed and grew roots on MS medium. Complete plantlets were obtained on MS basal medium. A total of 92% per cent of the plantlets survived and grew when transferred to the greenhouse. Agave micropropagation could supply the commercial plant demand, diminishing the gathering of seeds and plants of this endangered species from the wild.

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Authors and Affiliations

  1. Instituto de Investigaciones sobre Recursos Naturales, Universidad Michoacana de San Nicolás de Hidalgo, A.P. 12, Morelia Michoacán, 58041, Mexico

    Alejandro Martínez-Palacios

  2. Instituto de Geología, Universidad Nacional Autónoma de México, A.P. 70-296, Mexico D.F, 04510, Mexico

    M. Pilar Ortega-Larrocea

  3. Jardín Botánico, Instituto de Biología, Universidad Nacional Autónoma de México, A.P. 70-614, México D.F, 04510, Mexico

    Víctor M. Chávez & Robert Bye

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  1. Alejandro Martínez-Palacios
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  2. M. Pilar Ortega-Larrocea
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  3. Víctor M. Chávez
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  4. Robert Bye
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Correspondence to Alejandro Martínez-Palacios.

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Martínez-Palacios, A., Ortega-Larrocea, M.P., Chávez, V.M. et al. Somatic embryogenesis and organogenesis of Agave victoriae-reginae: Considerations for its conservation. Plant Cell, Tissue and Organ Culture 74, 135–142 (2003). https://doi.org/10.1023/A:1023933123131

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