Abstract
RAPD analysis was conducted in 22 cultivars of P. nigrum(black pepper) from South India and one accession each of P. longum and P. colubrinum. Twenty-four primers generated 372 RAPD markers of which 367 were polymorphic. Jaccard's similarity between pairs of accessions ranged between 0.11 and 0.66 with a mean of 0.38. Among P. nigrum cultivars, the similarity ranged between 0.20 and 0.66 and the mean was 0.42. The existence of wide genetic diversity as revealed in the present study is supported by earlier reports of extensive inter- and intrapopulation morphological variability in pepper cultivars from South India. UPGMA dendrogram and PCO plot revealed P. colubrinum to be most distant of the three species. Genetic proximity among P. nigrum cultivars could be related to their phenotypic similarities or geographical distribution. Greater divergence was observed among landraces than among advanced cultivars. Landraces grown in southern parts of coastal India and those grown in more northern parts were grouped in separate clusters of the dendrogram.
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References
Gamble J.S. 1925. Flora of Presidency of Madras. Botanical Survey of India, Calcutta, pp. 123-128. 2.
Hooker J.D. 1886. The Flora of British India. L. Reeve and Co., London, pp. 78-95. 5.
Ibrahim K.K., Pillay V.S. and Sasikumaran S. 1984. Discriminant functions in distinguishing between Travancore and Malabar cultivars of black pepper (Piper nigrum L.). Ind. Spices 21 and 22: 3-9.
Ibrahim K.K., Pillay V.S. and Sasikumaran S. 1985. Variability, heritability and genetic advance for certain qualitative characters in black pepper. Agric. Res. J. Kerala 23: 45-48.
Jose J. and Sharma A.K. 1985. Structure and behaviour of chromo-somes in Piper and Peperomia (Family Piperaceae). Cytologia. 50: 301-310.
Krishnamurthi A. 1969. The Wealth of India: Raw Materials. Council of Scientific and Industrial Research, New Delhi. 8.
Karp A. and Edwards K.J. 1995. Molecular techniques in the analysis of the extent and distribution of genetic diversity. In: Ayad W.G., Hodgkin T., Jaradat A. and Rao V.R. (eds), Molecular Genetic Techniques for Plant Genetic Resources. Report of an IPGRI Workshop 9-11 October 1995, Rome. International Plant genetic Resources Institute, Rome pp. 11-38., pp. 11-38.
Karp A., Issac P.G. and Ingram D.S. (eds) 1998. Molecular Tools for Screening Biodiversity. Chapman & Hall, London.
National Research Centre for Spices 1991. SpiceVarieties. Calicut, Kerala.
Parani M., Anand A. and Parida A. 1997. Application of RAPD fingerprinting in selection of micropropagated plants of Piper longum for conservation. Curr. Sci. 73: 81-83.
Pillay V.S., Ibrahim K.K. and Sasikumaran S. 1994. Improvement of black pepper. In: Chadha K.L. and Rethinum P. (eds), Adas vances in Horticulture Vol. 9. Malhotra Publishing House, New Delhi, pp. 293-306.
Ratnambal M.J., Ravindran P.N. and Nair M.K. 1985.Variability in black pepper cultivar 'Karimunda'. J. Plantation Crops 13: 154-157.
Ravindran P.N. and Nirmal Babu K. 1994. Genetic resources of black pepper. In: Chadha K.L. and Rethinum P. (eds), Advances in Horticulture Vol. 9. Malhotra Publishing House, New Delhi, pp. 99-120.
Ravindran P.N., Balakrishnan R. and Nirmal Babu K. 1997. Mor-phometrical studies on black pepper (Piper nigrum L.). 1. Cluster analysis of black pepper cultivars. J. Spices and Aromatic Crops 6: 9-20.
Rohlf F.J. 1993. NTSYS-pc numerical taxonomy and multivariate analysis system. Exerter Software, New York.
Sasikumar B., Chempakam B., George J.K., Remashree A.B., Devasahayam S., Dhamayanthi K.P.M. et al. 1999. Characterization of two interspecific hybrids of Piper. J. Hortic. Sci. Biotech. 74: 125-131.
Sneath P.H.A. and Sokal R.R. 1973. Numerical Taxonomy. W.H. Freeman, San Francisco, California.
Welsh J. and McClelland M. 1990. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213-7218.
Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A. and Tingey S.V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531-6535.
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Pradeepkumar, T., Karihaloo, J., Archak, S. et al. Analysis of genetic diversity in Piper nigrum L. using RAPD markers. Genetic Resources and Crop Evolution 50, 469–475 (2003). https://doi.org/10.1023/A:1023917809042
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DOI: https://doi.org/10.1023/A:1023917809042