Abstract
Genotoxicity and cytotoxicity of water samples from the creeks flowing through the area of radium mining waste storage into the Ukhta River and from this river were assessed using biological tests with Allium schoenoprasum L. Chemical analysis of the samples indicated that concentrations of parent heavy natural radionuclides, 210Pb, and 210Po do not exceed the allowable level. Of ten heavy metals, only Zn and Mn concentrations exceeded MACs. The results of biological tests provided evidence for the significantly increased genotoxicity of water samples. The mitotic index increased with an increase in the concentration of Zn ions and manifested an inverse dependence on the activity of 238U. The genotoxic effect linearly depended on Zn concentration. Possible mechanisms responsible for the induction of these biological effects are discussed.
Similar content being viewed by others
References
Adamova, L.I. and Taskaev, A.I., Quantitative Determination of 210Po and 210Pb in Soil and Plant Samples, in Migratsiya i biologicheskoe deistvie estestvennykh radionuklidov v usloviyakh severnykh biogeotsenozov (Migration and Biological Effects of Natural Radionuclides in Northern Biogeocenoses), Tr. Komi Fil. Akad. Nauk SSSR, Inst. Biol., no. 4, Syktyvkar, 1980, pp. 61–71.
Adelman, R., Saul, R. L., and Ames, B.N., Oxidative Damage to DNA: Relation to Species Metabolic Rate and Life Span, Proc. Natl. Acad. Sci. USA, 1988, vol. 85, pp. 2706–2708.
Aleksakhin, R.M., Arkhipov, N.P., Barkhudarov, R.M., et al., Tyazhelye estestvennye radionuklidy v biosfere. Migratsiya i biologicheskoe deistvie na populyatsii i biogeotsenozy (Natural Heavy Radionuclides in the Biosphere: Migration and Biological Effects on Populations and Biogeocenoses), Moscow: Nauka, 1990.
Bogoyavlenskii, L.N., The Ukhta Deposit of Radium, Dokl. Akad. Nauk SSSR, Ser. A, 1928, no. 14/15, p. 156.
Britt, A., DNA Repair Mechanism in Vegetable Cell, Radiat. Res., 1996, vol. 146, no. 5, pp. 1158–1172.
Dobrolyubskaya, T.S., The Fluorometric Method, in Analiticheskaya khimiya urana (Analytical Chemistry of Uranium), Moscow: Nauka, 1962, pp. 143–165.
Evseeva, T.I. and Geras'kin, S.A., Sochetannoe deistvie faktorov radiatsionnoi i neradiatsionnoi prirody na tradeskantsiyu (klon 02) [Combined Effect of Factors of Radiation and Nonradiation Nature on Spiderwort (Clone 02)], Yekaterinburg: Ural. Otd. Ross. Akad. Nauk, 2001.
Evseeva, T.I., Taskaev, A.I., and Kichigin, A.I., Vodnyi promysel (Trade in Aquatic Resources), Syktyvkar: Komi Nauch. Tsentr Ural. Otd. Ross. Akad. Nauk, 2000.
Evseeva, T.I., Geras'kin, S.A., and Khramova, E.I., Cytogenetic Effects of Separate and Combined Action of 232Th and Cd Nitrates on the Allium cepa Root Meristem Cells, Tsitologiya, 2001, vol. 43, no. 8, pp. 803–808.
Frizen, N.V., Lukovye Sibiri (sistematika, kariologiya, khorologiya) (Siberian Alliaceae: Taxonomy, Karyology, and Chorology), Novosibirsk: Nauka, 1988.
Geras'kin, S.A., Trends in the Development of Cytogenetic Effects from Small Doses of Ionizing Radiation, Abstract of Doctoral (Biol.) Dissertation, Obninsk, 1998.
Geras'kin, S.A., Zimina, L.M., Dikarev, V.G., Dikareva, N.S., Zimin, V.L., Vasil'ev, D.V., Blinova, L.D., Isaikina, N.V., and Nesterov, E.B., Bioindication-based Comparison of Anthropogenic Pollution Near a Radioactive-Waste Processing Facility and in the 30-km Control Area of the Chernobyl Nuclear Power Plant, Ekologiya, 2000, vol. 31, no. 4, pp. 300–303.
Grossman, S. and Turner, J.E., Mathematics for the Biological Sciences, New York; Macmillan, 1974. Translated under the title Matematika dlya biologov, Moscow: Vysshaya Shkola, 1983.
Kuznetsov, V.I. and Savvin, V.B., Sensitive Photometric Determination of Thorium with the Arsenazo-III Reagent, Radiokhimiya, 1961, vol. 3, no. 1, pp. 79–86.
Metzler, D.E., Biochemistry, New York: Academic, 1977. Translated under the title Biokhimiya, Moscow: Mir, 1980.
Normy radiatsionnoi bezopasnosti (NRB R 99): Gigienicheskie normativy (Radiation Safety Standards NRB R 99: Hygienic Norms), Moscow: Tsentr Sanitarno-Epidemiologicheskogo Normirovaniya, Gigienicheskoi Sertifikatsii i Ekspertizy Minzdrava Rossii, 1999.
Pausheva, Z.P., Praktikum po tsitologii rastenii (A Practical Course in Plant Cytology), Moscow: Kolos, 1980.
Petin, V.G., Zhurakovskaya, G.P., Pantyukhina, A.G., and Rassokhina, A.V., Small Doses and the Problems of Synergistic Interaction between Environmental Factors, Radiat. Biol. Radiekol., 1999, vol. 39, no. 1, pp. 113–126.
Polikarpov, G.G. and Tsytsugina, V.G., Trends in the Distribution of Chromosome Aberrations in Cells of Hydrobionts Exposed to the Influence of Ionizing Radiation and Environmental Chemical Mutagens, Radiat. Biol. Radiekol., 1993, vol. 33, no. 2, pp. 205–213.
Prakticheskoe posobie dlya rybovodov (Practical Manual for Fish Farmers), Moscow: Pishchevaya Promyshlennost', 1973.
Privezentsev, K.V., Milonova, I.N., and Bezlepkin, V.G., Evaluation of Toxic and Genotoxic Effects of Cd and Ni in Algotest and SOS-Chromotest, Usp. Sovrem. Biol., 1995, vol. 115, no. 6, pp. 759–764.
Radioekologicheskie issledovaniya v prirodnykh biogeotsenozakh (Radioecological Studies in Natural Biogeocenoses), Moscow: Nauka, 1972.
Rank, J. and Nielsen, M.H., Genotoxicity Testing of Wastewater Sludge Using the Allium cepa Anaphase–Telophase Chromosome Aberration Assay, Mutat. Res., 1998, no. 418, pp. 113–119.
Smirnova, S.A., Some Principles of Uranium Accumulation and Influence on Microalgae, Abstract of Doctoral (Biol.) Dissertation, Moscow, 1989.
Starik, I.E., Osnovy radiokhimii (Fundamentals of Radiochemistry), Leningrad: Nauka, 1969.
Udel'nova, T.M. and Yagodin, B.A., Zinc in the Life of Plants, Animals, and Man, Usp. Sovrem. Biol., 1993, vol. 113, no. 2, pp. 176–189.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Evseeva, T.I., Geras'kin, S.A., Shuktomova, I.I. et al. Comprehensive Analysis of Radioactive and Chemical Contamination of Water Bodies in the Area of Radium-Industry Waste Storage. Russian Journal of Ecology 34, 159–165 (2003). https://doi.org/10.1023/A:1023627317922
Issue Date:
DOI: https://doi.org/10.1023/A:1023627317922