Skip to main content
SpringerLink
Log in

Effect of Anthracene and Pyrene on Dehydrogenases Activity in Soils Exposed and Unexposed to PAHs

  • Published:
Water, Air, and Soil Pollution Aims and scope Submit manuscript

Abstract

The effect of anthracene and pyrene on dehydrogenases activitywas compared between freshly spiked soils and soils previouslyexposed to PAHs. Four different soils (two soils from anunpolluted rural area and two soils from highly pollutedindustrial areas) were spiked with anthracene and pyrene atthe levels of 1, 10, 100 and 400 mg Σ2PAH per kg of soil (laboratory experiments). The soil samples were moisted to60% of water holding capacity and incubated in the dark at 18-20 °C. Dehydrogenase activity was determined after 7 and30 days. The results indicate that long-term exposure of soilfrom an industrial area to high level of PAHs (Σ13PAH = 40 mg kg-1) caused the acclimatisation of soilmicroorganisms and lowered their sensitivity to these compounds.After the longer incubation period (30 days vs. 7 days)the soil microorganisms in the uncontaminated rural samples showed an increase in their tolerance to the freshly introduced PAH compounds; in the industrial soils the effect was less pronounced. However, this apparent adaptation of soil microorganisms may be also partly explained by the dissipation and/or ageing processes of anthracene and pyrene during incubation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alexander, M.: 2000, ‘Aging, bioavailability, and overestimation of risk from environmental pollutants’ Environ. Sci. Technol. 34, 4259-4265.

    Google Scholar 

  • ATSDR - U.S. Agency for Toxic Substances and Disease Registry Public Health Statement: 1990, Polycyclic Aromatic Hydrocarbons.

  • Bauer, J. E. and Capone, D. G.: 1988, ‘Effects of co-occuring hydrocarbons on degradation of individual polycyclic aromatic hydrocarbons in marine sediments slurries’ Appl. Environ. Microb. 54, 1649-1655.

    Google Scholar 

  • Baveye, P. and Bladon, R.: 1999, ‘Bioavailability of Organic Xenobiotics in the Environment’ in J. C. Block, V. V. Goncharuk and Ph. Baveye (eds), Bioavailability of Organic Xenobiotics in the Environment, NATO ASI Series, Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 227-248.

    Google Scholar 

  • Bleam R. D. and Cawthray M. K.: 1988, ‘Microbial Polynuclear Aromatic Hydrocarbon Degradation - Review and Case Histories’ in Wu, Yeun C., Technomic (eds), International Conference on Physical, Chemical and Biological Detoxification Hazardous Wastes, Lancaster, PA, U.S.A., pp. 868-882.

    Google Scholar 

  • Boesten, J. J. T. I.: 1993, ‘Bioavailability of organic chemicals in soil related to their concentration in the liquid phase: A review’ Sci. Total Environ. Supplement, 397-407.

  • Bulman, T. L., Lesage, S., Fowlie, P. J. A. and Webber, M. D.: 1985, ‘The Persistence of Polynuclear Aromatic Hydrocarbons in Soil’ Petroleum Association for Conservation of the Canadian Environment Report, Ottawa, Canada, No. 85-2.

  • Casida, L. E., Klein, D. A. and Santoro, T.: 1964, ‘Soil dehydrogenase activity’ Soil Sci. 98, 371-376.

    Google Scholar 

  • Chapman, P. M., Caldwell, R. S. and Chapman, P. F.: 1996, ‘A warning: NOECs are inappropriate for regulatory use’ Environ. Toxicol. Chem. 15, 77-79.

    Google Scholar 

  • Chiou, C. T.: 1989, ‘Theoretical Considerations of the Partition Uptake of Nonionic Organic Compounds by Soil Organic Matter’ inB. L. Sawhney and K. Brown (eds), Reactions and Movement of Organic Chemicals in Soils, Soil Science Society of America Special Publication, No. 22.

  • Gustafsson, Ö. and Gschwend, Ph. M.: 1997, ‘Soot as a Strong Partition Medium for Polycyclic Aromatic Hydrocarbons in Aquatic Systems’ in R. P. Eganhouse (ed.), Molecular Markers in Environmental Geochemistry, ACS Symposium Series 671, American Chemical Society, Washington, DC, pp. 365-381.

    Google Scholar 

  • Hatzinger, P. B. and Alexander, M.: 1995, ‘Effect of aging of chemicals in soil on their biodegradability and extractability’ Environ. Sci. Technol. 29, 537-545.

    Google Scholar 

  • Heitkamp, M. A. and Cerniglia, C. E.: 1987, ‘Effects of chemical structure and exposure on the microbial degradation of polycyclic aromatic hydrocarbons in freshwater and estuarine ecosystems’ Environ. Toxicol. Chem. 6, 535-546.

    Google Scholar 

  • Heitkamp, M. A. and Cerniglia, C. E.: 1988, ‘Mineralization of polycyclic aromatic hydrocarbons by a bacterium isolated from sediment below an oil field’ Appl. Environ. Microb. 54, 1612-1614.

    Google Scholar 

  • Howard, P. H. and Banerjee, S.: 1984, ‘Interpreting results from biodegradability tests of chemicals in water and soil’ Environ. Toxicol. Chem. 3, 551-562.

    Google Scholar 

  • Hu, J. G. and Johnson, R. L.: 1995, ‘Root growth, microbial activity and phosphatase activity in oilcontaminated, remediated and uncontaminated soils planted to barley and field pea’ Plant and Soil 173, 3-10.

    Google Scholar 

  • Hund, K. and Traunspunger, W.: 1994, ‘Ecotox-evaluation strategy for soil bioremediation exempli-fied for a PAH-contaminated site’ Chemosphere 29, 371-390.

    Google Scholar 

  • Jensen, J. and Folker-Hansen, P.: 1995, ‘Soil Quality Criteria for Selected Organic Compounds’ Working Report, No. 47, Danish Environmental Protection Agency.

  • Kalf, D. F., Crommentuin, T. and Van de Plassche, E. J.: 1997, ‘Environmental quality objectives for 10 polycyclic aromatic hydrocarbons (PAHs)’ Ecotox. Environ. Safety 36, 153-162.

    Google Scholar 

  • Mackay, D., Shiu, W. Y. and Ma, K. C.: 1992, Illustrated Handbook of Physical-chemical Properties and Environmental Fate for Organic Chemicals, Vol. II, Lewis Publishers, Boca Raton, Ann Arbor, London, Tokyo.

    Google Scholar 

  • Maliszewska-Kordybach, B.: 1993, Trwaloś´ćwielopierścieniowych węglowodorów aromatycznych w glebie, IUNG, H (4), Pulawy, 81 pp.

  • Maliszewska-Kordybach, B.: 1998, ‘The relationship between properties of PAHs and the rate of their disappearance from soils’ Toxicol. Environ. Chem. 66, 47-52.

    Google Scholar 

  • Maliszewska-Kordybach, B.: 1999, ‘Persistent Organic Contaminants in the Environment: PAHs as a Case Study’ in J. C. Block, V. V. Goncharuk and Ph. Baveye (eds), Bioavailability of Organic Xenobiotics in the Environment, NATO ASI Series, Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 3-34.

    Google Scholar 

  • Maliszewska-Kordybach, B. and Smreczak, B.: 2000, ‘Ecotoxicological activity of soils polluted with polycyclic aromatic hydrocarbons (PAHs) - Effect on plants’Environ. Technol. 21, 1099-1110.

    Google Scholar 

  • Maliszewska-Kordybach, B., Smreczak, B. and Martyniuk, S.: 2000, 'Wplyw wielopierścieniowych węglowodoròw aromatycznych na mikrobiologiczne wlaściwości gleb o zróźnicowanej kwasowości i zawartości substancji organicznych’ Roczniki Gleboznawcze 51, 5-18.

    Google Scholar 

  • Martyniuk, S., Zieba, S. and Mackowiak, Cz.: 1998, ‘Relationship between enzymatic activity of soil and yields of spring barley in a long-term field experiment’ Zesz. Nauk. Akad. Rol. Wrocl. 332, 31-38.

    Google Scholar 

  • Nam, K., Chung, N. and Alexander, M.: 1998, ‘Relationship between organic matter content of soil and the sequestration of phenanthrene’ Environ. Sci. Technol. 32, 3785-3788.

    Google Scholar 

  • Northcott, G. L. and Jones, K. C.: 2000, ‘Experimental approaches and analytical techniques for determining organic compound bound residues in soil and sediment’ Environ. Poll. 108, 19-43.

    Google Scholar 

  • Ostrowska, A., Gawlinski, K. and Szczubialka, Z.: 1991, Metody analizy i oceny wlasciwosci gleb i roslin, Instytut Ochrony Roslin, Warszawa.

    Google Scholar 

  • Pankhurst, C. E., Rogers, S. L. and Gupta, V. S. R.: 1998, ‘Microbial Parameters for Monitoring Soil Pollution’ in J. M. Lynch and A. Wiseman (eds), Environmental Biomonitoring: The Biotechnology Ecotoxicology Interface, Cambridge University Press, Cambridge, pp. 46-68.

    Google Scholar 

  • Reid, B. J., Jones, K. C. and Semple, K. T.: 2000, ‘Bioavailability of persistent organic pollutants in soils and sediments - A perspective on mechanisms, consequences and assessment’ Environ. Pollut. 108, 103-112.

    Google Scholar 

  • Remde, A. and Hund, K.: 1994, ‘Response of soil autotrophic nitrification and soil respiration to chemical pollution in long-term experiments’ Chemosphere 29, 391-404.

    Google Scholar 

  • Ren, L., Zieler, L. F., Dixon, D. G. and Greenberg, B. M.: 1996, ‘Photoinduced effects of polycyclic aromatic hydrocarbons on Brassica napus (Canola) during germination and early seedling development’ Ecotox. Environ. Safety 33, 73-80.

    Google Scholar 

  • Riepert, F. and Wilke, B. M.: 1999, ‘Soil Quality - Guidance on the Ecotoxicological Characterization of Soils and Soil Materials’ Document ISO/15799.

  • Rossel, D., Tarradellas, J., Bitton, G. and Morel, J. L.: 1997, ‘Use of Enzymes in Soil Ecotoxicology: A Case of Dehydrogenase and Hydrolitic Enzymes’ in J. Tarradellas, G. Bitton, and D. Rossel (eds), Soil Ecotoxicology, Lewis Publishers, Boca Raton, New York, London, Tokyo, pp. 179-206.

    Google Scholar 

  • Sabljic, A., Gusten, H., Verhaar, H. and Hermens, J.: 1995, ‘QSAR modelling of soil sorption. Improvements and systematics of log Koc vs. log Kow correlations’ Chemosphere 31, 4489-4514.

    Google Scholar 

  • Sijm, D., Kraaij, R. and Belfroid, A.: 2000, ‘Bioavailability in soil and sediment: Exposure of different organisms and approaches to study it’ Environ. Poll. 108, 113-119.

    Google Scholar 

  • Sikkema, J., De Bont, J. A. M. and Poolman, B.: 1995, ‘Mechanisms of membrane toxicity of hydrocarbons’ Microbiol. Review 59(2), 201-222.

    Google Scholar 

  • Smreczak, B. and Maliszewska-Kordybach, B.: 1999, ‘Effects of PAHs and Heavy Metals on Activity of Soil Microflora’ in J. C. Block, V. V. Goncharuk and Ph. Baveye (eds), Bioavailability of Organic Xenobiotics in the Environment, NATO ASI Series, Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 377-380.

    Google Scholar 

  • Sverdrup, L. E.: 2001, ‘Toxicity of Tar Constituents in Terrestrial Ecosystem. Effects of Eight Polycyclic Aromatic Compounds on Terrestrial Plants, Soil Invertebrates andMicroorganisms’ Ph.D. Thesis, Faculty of Mathematics and Natural Sciences, University of Oslo.

    Google Scholar 

  • Swartz, R. C., Schults, D.W., Ozretich, R. J., Lamberson, J. O., Cole, F. A., DeWitt, T. H., Redmond, M. S. and Ferraro, S. P.: 1995, ΣPAH: A model to predict the toxicity of polynuclear aromatic hydrocarbon mixtures in field-collected sediments’ Environ. Toxicol. Chem. 11, 1977-1987.

    Google Scholar 

  • Torstensson, L.: 1997, ‘Microbial Assays in Soil’ in J. Tarradellas, G. Bitton, and D. Rossel (eds), Soil Ecotoxicology, Lewis Publishers, Boca Raton, New York, London, Tokyo, pp. 207-233.

    Google Scholar 

  • Turek-Szytow, J.: 2000, ‘Kontrola i ocena wplywu wielopierścieniowych węglowodorów aromatycznych (WWA) na proces rekultywacji gleb zanieczyszczonych tymi zwiazkami’ Ph.D. Thesis, Politechnika Śl ąska, Gliwice.

    Google Scholar 

  • Van de Leemkule, M. A., Van Hesteren, S. and Pruiksma, M. A.: 1998, ‘Minimum Soil Quality: A Use-based Approach from Ecological Perspective. Part 2: Immobile Organic Micro-pollutants’ Report TCB R09, Technical Soil Protection Committee, The Netherlands.

    Google Scholar 

  • VROM: 1995, Leidraad Bodembescherming, Afl. 10.

  • Weissenfels, W. D., Klewer, H.-J. and Langhoff, J.: 1992, ‘Adsorption of polycyclic aromatic hydrocarbons (PAHs) by soil particles: Influence on biodegradability and biotoxicity’ Appl. Microbiol. Biotechnol. 36, 689-696.

    Google Scholar 

  • Wetzel, A.: 1998, ‘Advances in Biomonitoring: Sensitivity and Reliability in PAH-contaminated Soil’ in J. M. Lynch and A. Wiseman (eds), Environmental Biomonitoring: The Biotechnology Ecotoxicology Interface, Cambridge University Press, Cambridge, pp. 27-45.

    Google Scholar 

  • Wild, S. R. and Jones, K. C.: 1995, ‘Polynuclear aromatic hydrocarbons in the United Kingdom environment: A preliminary source inventory and budget’ Environ. Pollut. 88, 91-108.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Soil Science, Erosion and Land Conservation, Institute of Soil Science and Plant Cultivation, Puławy, Poland

    A. Klimkowicz-Pawlas & B. Maliszewska-Kordybach

Authors
  1. A. Klimkowicz-Pawlas
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Maliszewska-Kordybach
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. Maliszewska-Kordybach.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klimkowicz-Pawlas, A., Maliszewska-Kordybach, B. Effect of Anthracene and Pyrene on Dehydrogenases Activity in Soils Exposed and Unexposed to PAHs. Water, Air, & Soil Pollution 145, 169–186 (2003). https://doi.org/10.1023/A:1023619023030

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1023619023030

Search

Navigation