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Optimization of Synthesis of Ethyl Isovalerate Using Rhizomucor miehei Lipase

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Abstract

Immobilized lipase from Rhizomucor miehei (Lipozyme IM-20) was used to catalyze the esterification reaction between isovaleric acid and ethanol to synthesize ethyl isovalerate in n-hexane. Response surface methodology based on a four-variable, five-level, central composite rotatable design was employed to optimize four important reaction variables—enzyme/substrate (E/S) ratio, substrate concentration, incubation time, and temperature—affecting the synthesis of ethyl isovalerate. The optimum conditions predicted for achieving maximum ester yield (500 mM) are as follows: E/S ratio, 48.41 g/mol; substrate concentration, 1 M; reaction time, 60 h; and temperature, 60°C. The predicted value matched well with the experimentally obtained value of 487 mM.

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  1. Fermentation Technologies and Bioengineering Department, Central Food Technological Research Institute, Mysore, 570 013, India

    G. V. Chowdary & S. G. Prapulla

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  1. G. V. Chowdary
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  2. S. G. Prapulla
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Chowdary, G.V., Prapulla, S.G. Optimization of Synthesis of Ethyl Isovalerate Using Rhizomucor miehei Lipase. Applied Biochemistry and Microbiology 39, 243–248 (2003). https://doi.org/10.1023/A:1023567325422

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