Abstract
The effects of rye chromosomes 1R and 5R on androgenesis in cultured anthers of wheat–rye substitution lines was studied as dependent on the cultivar origin of the rye chromosomes and on the wheat genome (A or D) subjected to substitution. Chromosome 1R stimulated embryogenesis in anther cultures, while chromosome 5R suppressed it regardless of whether the corresponding wheat chromosomes were substituted in the A or D genome. The effect of chromosome 1R on embryogenesis proved to depend on its cultivar origin. Along with rye chromosome 1R, wheat chromosome 1A was shown to substantially affect total seedling regeneration. Regeneration of green seedlings was dramatically affected both by rye chromosome 1R and by wheat chromosome 1D. The results supported the published data that individual androgenesis parameters (embryogenesis, total plant regeneration, green plant regeneration) are controlled by different genetic mechanisms.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bajaj, Y.P.S., In Vitro Production of Haploids and Their Use in Cell Genetics and Plant Breeding, Biotechnology in Agriculture and Forestry, Bajaj, Y.P.S., Ed., Berlin: Springer-Verlag, 1990, pp. 4–43.
Holme, I.B., Olesen, A., Hansen, J.P., and Andersen, S.B., Anther and Isolated Microspore Culture Response of Wheat Lines from Northwestern and Eastern Europe, Plant Breed., 1999, vol. 118, pp. 111–117.
Andersen, S.B., Due, I.K., and Olesen, A., The Response of Anther Culture in Genetically Wide Material of Winter Wheat (Triticum aestivum L.), Plant Breed., 1987, vol. 99, pp. 181–186.
Agache, S., De Buyeser, J., and Snape, J., Studies of the Genetic Relationship between Anther Culture and Somatic Tissue Culture Abilities in Wheat, Plant Breed., 1988, vol. 100, no. 1, pp. 26–33.
Agache, S., Bachelier, B., De Buyser, J., et al., Genetic Analysis of Anther Culture Response in Wheat Using Aneuploid, Chromosome Substitution and Translocation Lines, Theor. Appl. Genet., 1989, vol. 77, no. 1, pp. 7–11.
Szakacs, E., Kovacs, G., Pauk, J., and Barnabas, B., Substitution Analysis of Callus Induction and Plant Regeneration from Anther Culture in Wheat (Triticum aestivum L.), Plant Cell Rep., 1988, vol. 7, pp. 127–129.
Martinez, I., Bernard, M., Nicolas, P., and Bernard, S., Study of Androgenetic Performance and Molecular Characterization of a Set of Wheat–Rye Addition Lines, Theor. Appl. Genet., 1994, vol. 89, pp. 982–990.
Henry, Y. and De Buyser, J., Effect of the 1B/1R Translocation on Anther-Culture Ability in Wheat (Triticum aestivum L.), Plant Cell Rep., 1985, vol. 4, pp. 307–310.
Foroughi-Wehr, B. and Zeller, F.J., In Vitro Microspore Reaction of Different German Wheat Cultivars, Theor. Appl. Genet., 1990, vol. 79, pp. 77–80.
Dobrovol'skaya, O.B., Pershina, L.A., Kravtsova, L.A., et al., The Effect of Rye Chromosomes on Adrogenesis in Wheat–Rye (Triticum aestivum L. Cultivar Saratovskaya 29/Secale cereale L. Cultivar Onokhoiskaya) Substitution Lines and Tritikale, Genetika (Moscow), 2001, vol. 37, no. 5, pp. 624–630.
Shchapova, A.I. and Kravtzova, L.A., The Production of Wheat–Rye Substitution Line by Using the Giemsa Staining Technique, Cereal Res. Commun., 1982, vol. 10, no. 1, pp. 33–39.
Chuang, C.C., Ouyang, J.W., and Chia, H., A Set of Potato Media for Wheat Anther Culture, Proc. Symp. Plant Tissue Culture, Peking: Sci., 1978, pp. 51–56.
Gamborg, O.L. and Eveleigh, D.E., Culture Methods and Detection of Gluconases in Suspension in Culture of Wheat and Barley, Can. J. Bot., 1968, vol. 46, no. 5, pp. 417–421.
Lakin, G.F., Biometriya (Biometry), Moscow: Vysshaya Shkola, 1980.
Zheng, M.Y. and Konzak, C.F., Effect of 2,4-Dichlorophenoxyacetic Acid on Callus Induction and Plant Regeneration in Anther Culture of Wheat (Triticum aestivum L.), Plant Cell Rep., 1999, vol. 19, pp. 69–73.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dobrovolskaya, O.B., Pershina, L.A., Kravtsova, L.A. et al. Comparative Effects of Rye Chromosomes 1R and 5R on Androgenesis in Cultured Anthers of Wheat–Rye Substitution Lines as Dependent on the Line Origin. Russian Journal of Genetics 39, 467–470 (2003). https://doi.org/10.1023/A:1023378219158
Issue Date:
DOI: https://doi.org/10.1023/A:1023378219158