Abstract
An electrophoretic band with butyrylcholinesterase activity was detected in 71 CHE2 C5+ and 378 CHE2 C5− individuals and was named C4/5 in view of its similar mobility to either C4 or C5, depending on the pH of the agar gel used. The present data suggest that C4/5 is a heterologous complex of butyrylcholinesterase. Although the C4/5 band may have the same mobility as C5, depending on the conditions of electrophoresis, our hypothesis is that these two bands result from the association of BChE with different molecules.
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Alcântara, V. M. (2000). Fenótipos da butirilcolinesterase e suas relações com dados antropométricos, bioquímico-hormonais e pressão arterial em obesos e na população geral de Curitiba, PR, DSc Thesis, Department of Genetics, Federal University of Paraná, Curitiba, Brazil, 228 pp.
Alcântara, V. M., Lourenço, M. A. C., Salzano, F. M., Petzl-Erler, M. L, Coimbra Jr., C. E. A., Santos, R. V., & Chautard-Freire-Maia, E. A. (1995). Butyrylcholinesterase polymorphisms (BCHE and CHE2 loci) in Brazilian Indian and admixed populations. Hum. Biol. 67:717.
Alcântara, V. M., Rodrigues, L. C., Oliveira, L. C., & Chautard-Freire-Maia, E. A. (2001). Association of the CHE2 locus with body mass index and butyrylcholinesterase activity. Hum. Biol. 73:587.
Ashton, G. C., & Simpson, N. E. (1966). C5 types of serum cholinesterase in a Brazilian population. Am. J. Hum. Genet. 18:438.
Chautard-Freire-Maia, E. A., Primo-Parmo, S. L., Picheth, G., Lourenço, M. A. C., & Vieira, M. M. (1991). The C5 isozyme of serum cholinesterase and adult weight. Hum. Hered. 41:330.
Delbrück, A., & Henkel, E. (1979). A rare genetically determined variant of pseudocholinesterase in two German families with high plasma enzyme activity. Eur. J. Biochem. 99: 65.
Erdös, E. G., Debay, C. R., & Westerman, M. P. (1960). Arylesterases in blood: Effect of calcium and inhibitors. Biochem. Pharmacol. 5:173.
Gallango, M. L., & Arends, T. (1969). Phenotypical variants of pseudocholinesterase in myeloma patients. Humangenetik 7:104.
Harris, H., Hopkinson, D. A., & Robson, E. B. (1962). Two-dimensional electrophoresis of pseudocholinesterase components in human serum. Nature 196:1296.
Harris, H., Hopkinson, D. A., Robson, E. B., & Whittaker, M. (1963). Genetical studies on a new variant of serum cholinesterase detected by electrophoresis. Ann. Hum. Genet. 26:359.
Juul, P. (1968). Human plasma cholinesterase isoenzymes. Clin. Chim. Acta 19:205.
Kalow, W., & Davies, R. O. (1958). The activity of various esterase inhibitors towards atypical human serum cholinesterase. Biochem. Pharmacol. 1:183.
La Motta, R. V., Mccomb, R. B., Noll, C. R. Jr., Wetstone, H. J., & Reinfrank, R. F. (1968). Multiple forms of serum cholinesterase. Arch. Biochem. Biophys. 124:299.
Masson, P. (1978). Formes moleculaires multiples et structure quaternaire de la butyrylcholinesterase du serum humain, DSc Thesis, Université Claude Bernard, Lyon, France.
Masson, P. (1979). Formes moleculaires multiples de la butyrylcholinesterase du plasma humain. Biochim. Biophys. Acta 578:493.
Masson, P. (1991). Molecular heterogeneity of human plasma cholinesterase. In Massoulié, J., Barnard, E., Chatonnet, A., Bacou, F., Doctor, B. P., Quinn, D. M. (eds.), Cholinesterases. Structure, Function, Mechanisms, Genetics and Cell Biology, American Chemical Society, Washington, DC, pp. 42–45.
Masson, P., Froment, M. T., & Audras, J.-C. (1990). Molecular characterization of the C5 human plasma cholinesterase variant (CHE2). In Abstracts, Third International Meeting on cholinesterases, p. 181.
Petzl, M. L., & Primo-Parmo, S. L. (1979). Eletroforese de hemoglobina, haptoglobina, anidrase carbônica II e esterase D em gel de amido de milho comercial (Maizena). Cienc. Cult. 31:896.
Poulik, M. D. (1957). Starch gel electrophoresis in a discontinuous system of buffers. Nature 180:1477.
Primo-Parmo, S. L., Vieira, M. M., Lourenço, M. A. C., Picheth, G., & Chautard-Freire-Maia, E. A. (1990). Modifications of the C5 isozyme of human serum cholinesterase by electrophoresis. In Abstracts, Third International Meeting on cholinesterases, p. 182.
Van Ros, G, and Druet, R. (1966). Uncommon electrophoretic patterns of serum cholinesterase (pseudocholinesterase). Nature 212: 543.
Van Ros, G, and Vervoort, T. (1973). Frequencies of the “atypical” and C5 variants of serum cholinesterase in Zairians and Belgians. Detection of the C5 variant by agar gel electrophoresis with an acid buffer. Ann. Soc. Belg. Méd. Trop. 53:633.
Yamamoto, K., Morito, F., Motomura, M., Kaneoka, H., & Sadai, T. (1986). A case of familial hyper-cholinesterasemia associated with isozyme variant band. Gastroentol. Jpn. 21:379.
Yamamoto, K., Morito, F., Setouguchi, Y., Fujii, S., & Kariya, T. (1987). Characterization of serum cholinesterase in familial hyper-cholinesterasemia associated with an isozyme variant band. Gastroentol. Jpn. 22:187.
Yoshida, A., & Motulsky, A. G. (1969). A pseudocholinesterase variant (E Cynthiana) associated with elevated plasma enzyme activity. Am. J. Hum. Genet. 21:486.
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Souza, R.L.R., Furtado, L., Diniz, A.C.P. et al. Studies on a Heterologous Complex Formed by Human Butyrylcholinesterase. Biochem Genet 41, 141–150 (2003). https://doi.org/10.1023/A:1023373526230
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DOI: https://doi.org/10.1023/A:1023373526230