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Species Differences in the Susceptibility of Erythrocytes Exposed to Free Radicals In Vitro

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Abstract

The susceptibility of human, cow, pig, sheep and rabbit erythrocytes to free radicals (peroxyl radicals) generated in vitro by 2,2′-azo-bis(2-amidinopropane) hydrochloride (AAPH) was evaluated by means of a haemolysis test and expressed as the time to 50% of maximal haemolysis (HT50). The most sensitive to damage by free radicals appeared to be the erythrocytes of pigs and sheep, their HT50 values being (mean±SEM) 85.1±1.28 min and 89.0±1.31 min, respectively. Human erythrocytes and those of cows and rabbits were about twice as resistant, their HT50 values being (mean±SEM) 174.3±1.53 min, 181.2±1.22 min and 183.4±2.54 min, respectively. Pig and sheep erythrocytes used in the haemolysis test provided an indication of the antioxidant status in a shorter time (2.5 h versus 4.5 h) than those of the other species studied. The results indicate that the HT50 test may be a convenient alternative to the osmotic resistance test for defining the antioxidant resistance of erythrocytes.

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  1. Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Department of Hormonal Action Mechanisms, ul. Grunwaldzka 250, 60–166, Poznań, Poland

    E. Brzezińska-Ślebodzińska

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Brzezińska-Ślebodzińska, E. Species Differences in the Susceptibility of Erythrocytes Exposed to Free Radicals In Vitro . Vet Res Commun 27, 211–217 (2003). https://doi.org/10.1023/A:1023344607691

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