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Histopathological assessment of gonadal tissue in wild fishes

Abstract

Histology offers a powerful tool in the study of reproductive health of fishes. It is routinely used for sex verification, identifying stage of development, documenting presence of intersex, tumors, parasites and other abnormalities and quantifying atresia. It can also be used for more subtle changes such as thickness of the vitelline envelope at various stages, yolk appearance, necrosis of sperm, and Sertoli cell proliferation. Gonadal histology, in conjunction with hormone and vitellogenin measurements, morphological and fecundity studies, can provide insights into the effects of various environmental stressors on reproductive health. However, much research, both field and laboratory, is needed to understand cause and effect for observed changes and to understand the meaning of many of the histological observations made in field studies, in terms of reproductive success of fish populations.

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References

  • Ahsan, S.N. 1966. Cyclical changes in the testicular activity of lake chub, Couesius plumbeus. Can. J. Zool. 44: 149–159.

    Google Scholar 

  • Arukwe, A., Knudsen, F.R. and Goksøyr, A. 1997. Fish zona radiata (eggshell) protein: a sensitive biomarker for environmental estrogens. Environ. Health Perspect. 105: 418.

    PubMed  CAS  Google Scholar 

  • Atz, J.W. 1964. Intersexuality in fishes. In: Intersexuality in Vertebrates Including Man, pp. 145–232. Edited by C.N. Armstrong and A.J. Marshall. Academic Press, NY.

    Google Scholar 

  • Balon, E.K. 1975. Reproductive guilds of fishes: a proposal and definitions. J. Fish. Res. Bd. Can. 32: 821–864.

    Google Scholar 

  • Balon, E.K. 1981. Additions and amendments to the classification of reproductive styles in fishes. Env. Biol. Fish. 6: 377–389.

    Article  Google Scholar 

  • Bandyopadhyay, M.P. and Aditya, A.K. 1998. Chemical induced changes in percentage and sizes of diameter in the ovarian oocytes of Anabas testudineus (Bloch). J. Freshwat. Biol. 10: 75–82.

    Google Scholar 

  • Barry, C.E., Wainscott, M.R., Foott, S.J. and Patiño, R. 2001. Gonadal macrophage aggregates: novel biomarker of contaminant exposure and effect in male fishes? Proceedings of the Society of Environmental Toxicology and Chemistry 2001 Annual meeting, Nov. 11–15, Baltimore, MD, 222 pp.

  • Berlinsky, D.L., Jackson, L.F., Smith, T.I.J. and Sullivan, C.V. 1995. The annual reproductive cycle of the white bass Morone chrysops. J. World Aqua. Soc. 26: 252–260.

    Google Scholar 

  • Besseau, L. and Faliex, E. 1994. Resorption of unemitted gametes in Lithognathus mormyrus (Sparidae, Teleostei): a possible synergic action of somatic and immune cells. Cell. Tissue Res. 276: 123–132.

    Article  PubMed  CAS  Google Scholar 

  • Bhatti, M.N. and Al-Daham, N.K. 1978. Annual cyclical changes in the testicular activity of a freshwater teleost, Barbus luteus (Heckel) from Shatt-Al-Arab, Iraq. J. Fish Biol. 13: 321–326.

    Article  Google Scholar 

  • Blazer, V.S., Facey, D.E., Fournie, J.W., Courtney, L.A. and Summers, J.K. 1994. Macrophage aggregates as indicators of environmental stress. In: Modulators of Fish Immune Responses, Vol. 1, pp. 169–185. Edited by J.S. Stolen J.S. and T.C. Fletcher. SOS Publications, Fair Haven, NJ.

    Google Scholar 

  • Blazer, V.S., Fournie, J.W. and Weeks-Perkins, B.A. 1997. Macrophage aggregates: Biomarker for immune function in fish? In: Environmental Toxicology and Risk Assessment: Modeling and Risk Assessment (Sixth Volume), pp. 360–375. F.J. Dwyer, T.R. Doane and M.L. Hinman. American Society for Testing and Materials, Philadelphia, PA.

    Google Scholar 

  • Borg, B. and Van Veen, T.H. 1982. Seasonal effects of photoperiod and temperature on the ovary of the three-spined stickleback, Gasterosteus aculeatus L. Can. J. Zool. 60: 3387–3393.

    Google Scholar 

  • Bortone, S.A. and Cody, R.F. 1999. Morphological masculinization in poeciliid females from a paper mill effluent receiving tributary of the St. Johns River, Florida, USA. Bull. Environ. Contam. Toxicol. 63: 150–156.

    Article  PubMed  CAS  Google Scholar 

  • Braekevelt, C.R. and McMillan, D.B. 1967. Cyclic changes in the ovary of the brook stickelback, Eucalia inconstans (Kirtland). J. Morphol. 123: 373–396.

    Article  PubMed  CAS  Google Scholar 

  • Breder, C.M., Jr., and Rosen, D.E. 1966. Modes of reproduction in fishes. Natural History Press, Garden City, NJ. 941 pp.

    Google Scholar 

  • Burke, M.G. and Leatherland, J.F. 1984. Seasonal changes in testicular histology of brown bullheads, Ictalurus nebulosus Lesueur. Can. J. Zool. 62: 1185–1194.

    Article  Google Scholar 

  • Budd, J., Schroder, J.D., and Dukes, K.D. 1975. Tumors of yellow perch. In: The Pathology of Fishes, pp. 895–906. Edited by W.E. Ribeln and G. Migaki. University of Wisconsin Press, Madison, WI

    Google Scholar 

  • Chan, S.T.H., Wright, A. and Phillips, J.G. 1967. The atretic structures in the gonads of the rice-field eel (Monopterus albus) during natural sex-reversal. J. Zool. London 151: 129–141.

    Article  Google Scholar 

  • Cheek, A.O., Brouwer, T.H., Carroll, S., Manning, S., McLachlan, J.A. and Brouwer, M. 2001. Experimental evaluation of vitellogenin as a predictive biomarker for reproductive disruption. Environ. Health Perspect. 109: 681–690.

    PubMed  CAS  Google Scholar 

  • Chitwood, M. and Lichtenfels, J.R. 1972. Identification of parasitic metazoa in tissue sections. Exp. Parasitol. 32: 407–519.

    Article  PubMed  CAS  Google Scholar 

  • Cross, J.N. and Hose, J.E. 1988. Evidence for impaired reproduction in white croaker Genyonemus lineatus from contaminated areas off southern California. Mar. Environ. Res. 24: 185–188.

    Article  CAS  Google Scholar 

  • DeVlaming, V. 1983. Oocyte development patterns and hormonal involvements among teleosts. In: Control Processes in Fish Physiology, pp. 176–210. Edited by J.C. Rankin, T.J. Pitcher and R.T. Duggan. John Wiley & Sons, NY.

    Google Scholar 

  • DeVlaming, V., Grossman, G. and Chapman, F. 1982. On the use of the gonadosomatic index. Comp. Biochem. Physiol. 73A: 1–39.

    Google Scholar 

  • Denslow, N.D., Chow, M.C., Kroll, K.J. and Green, L. 1999. Vitellogenin as a biomarker of exposure for estrogen or estrogen mimics. Ecotoxicol. 8: 385–398.

    Article  CAS  Google Scholar 

  • Down, N.E. and Leatherland, J.F. 1989. Histopathology of gonadal neoplasms in cyprinid fish from the lower Great Lakes of North America. J. Fish Dis. 12: 415–437.

    Article  Google Scholar 

  • Ellis, A.E. 1980. Antigen-trapping in the spleen and kidney of plaice Pleuronectes platessa L. J. Fish Dis. 3: 413–426.

    Article  Google Scholar 

  • Ellis, A.E., Munroe, A.L.S. and Roberts, R.J. 1976. Defense mechanisms in fish. 1. A study of the phagocytic system and the fate of intraperitoneally injected material in the plaice (Pleuronectes platessa). J. Fish Biol. 8: 67–78.

    Article  Google Scholar 

  • Flammarion, P., Brion, F., Babut, M., Garric, J., Migeon, B., Noury, P., Thybaud, E., Tyler, C.R. and Palazzi, X. 2000. Induction of fish vitellogenin and alterations in testicular structue: Preliminary results of estrogenic effects in chub (Leuciscus cephaus). Ecotoxicol. 9: 127–135.

    Article  CAS  Google Scholar 

  • Fournie, J.W., Summers, J.K., Courtney, L.A., Engle, V.D. and Blazer, V.S. 2001. Utility of splenic macrophage aggregates as an indicator of fish exposure to degraded environments. J. Aquat. Anim. Health 13: 105–116.

    Article  Google Scholar 

  • Gardiner, C.H., Fayer, R. and Dubey, J.P. 1998. An Atlas of Protozoan Parasites in Animal Tissues. Registry of Veterinary Pathology, Armed Forces Institute of Pathology, American Registry of Pathology, Washington, DC, 84 pp.

    Google Scholar 

  • Gardiner, C.H. and Poynton, S.L. 1999. An Atlas of Metazoan Parasites in Animal Tissues. Registry of Veterinary Pathology, Armed Forces Institute of Pathology, American Registry of Pathology, Washington, DC, 63 pp.

    Google Scholar 

  • Goodbred, S.L., Gilliom, R.J., Gross, T.S., Denslow, N.P., Bryant, W.L. and Schoeb, T.R. 1997. Reconnaissance of 17β-estradiol, 11–ketotestosterone, vitellogenin, and gonadal histopathology in common carp of United States streams: Potential for contaminant-induced endocine disruption. U.S. Geological Survey Open-File Report 96–627, Sacramento, California, 47 pp.

  • Grier, H. 1981. Cellular organization of the testis and spermatogenesis in fishes. Amer. Zool. 21: 345–357.

    Google Scholar 

  • Grier, H. 2000. Ovarian germinal epithelium and folliculogenesis in the common snook, Centropomus undecimalis (Teleostei: Centropomidae). J. Morphol. 243: 265–281.

    Article  PubMed  CAS  Google Scholar 

  • Grier, H.J. and Taylor, R.G. 1998. Testicular maturation and regression in common snook. J. Fish Biol. 53: 521–542.

    Article  Google Scholar 

  • Guraya, S.S. 1986. The Cell and Molecular Biology of Fish Oogenesis. In: Monographs in Developmental Biology Vol. 18, 223 pp. Edited by H.W. Sauer. Karger, Basel.

    Google Scholar 

  • Hawkins, W.E., Fournie, J.W., Ishikawa, T. and Walker, W.W. 1996. Germ cell neoplasms in Japanese medaka. J. Aquat. Anim. Health 8: 120–129.

    Article  Google Scholar 

  • Hemming, J.M., Waller, W.T. and Ammann, L.P. 2001. Assessment of wastewater effluent estrogenicity in North Central Texas using the vitellogenin biomarker in male fathead minnows (Pimephales promelas Rafinesque). Texas J. Sci. 53: 335–344.

    CAS  Google Scholar 

  • Henderson, N.E. 1962. The annual cycle in the testis of the eastern brook trout, Salvelinus fontinalis (Mitchell). Can. J. Zool. 40: 631–641.

    Google Scholar 

  • Hinton, D.E., Baumann, P.C., Gardner, G.R., Hawkins, W.E., Hendricks, J.D., Murchelano, R.A. and Okihiro, M.S. 1992. Histopathologic biomarkers. In: Biomarkers Biochemical, Physiological and Histological Markers of Anthropogenic Stress, pp. 155–210. Edited by R.J. Huggett, R.A. Kimerle, P.M. Mehrle, Jr., and H.L. Bergman. Lewis Publishers, Chelsea, MI.

    Google Scholar 

  • Hinton, D.E. and Laurén, D.J. 1990. Integrative histopathological approaches to detecting effects of environmental stressors in fishes. Am. Fish. Soc. Symp. 8: 51–66.

    Google Scholar 

  • Htun-Han, M. 1978. The reproductive biology of the dab Limanda limanda (L.) in the North Sea: Seasonal changes in the ovary. J. Fish Biol. 13: 351–359.

    Article  Google Scholar 

  • Jackson, L.F. and Sullivan, C.V. 1995. Reproduction of white perch: Annual gametogenic cycle. Trans. Amer. Fish. Soc. 124: 563–577.

    Article  Google Scholar 

  • Jobling, S., Nolan, M., Tyler, C.B., Brighty, G. and Sumpter, J.P. 1998. Widespread sexual disruption in wild fish. Environ. Sci. Technol. 32: 2498–2506.

    Article  CAS  Google Scholar 

  • Johnson, L.L., Casillas, E., Collier, T.K., McCain, B.B. and Varanasi, U. 1988. Contaminant effects on ovarian development in English sole (Parophrys vetulus) from Puget Sound, Washington. Can. J. Fish. Aquat. Sci. 45: 2133–2146.

    Article  CAS  Google Scholar 

  • Johnson, L.L., Sol, S.Y., Lomax, D.P., Nelson, G.M., Sloan, C.A., and Casillas, E. 1997. Fecundity and egg weight in English sole, Pleuronectes vetulus, from Puget Sound, Washington: influences of nutritional status and chemical contaminants. Fish. Bull. 95: 231–249.

    Google Scholar 

  • Kendall, R.J., Dickerson, R.I., Giesy, J.P., and Suk, W.A. 1998. Principles and Processes for Evaluating Endocrine Disruption in Wildlife. Society for Environmental Toxicology and Chemistry Press, Pensacola, FL. 515 pp.

    Google Scholar 

  • Kime, D.E. 1998. Endocrine Disruption in Fish. Kluwer Academic Publishers, Norwell.

    Google Scholar 

  • Leatherland, J.F., Down, N.E., Falkmer, S. and Sonstegard, R.A. In Press. Endocrine glands and reproductive system. In: Pathobiology of Spontaneous and Induced Neoplasms in Fishes: Comparative Characterization, Nomenclature and Literature. Edited by C.J. Dawe, J.C. Harshbarger, S.R. Wellings and J.D. Standberg. Academic Press, NY.

  • Lucano-Ramirez, G., Cruz, M. V.-S., Ruiz-Ramirez, S. and López-Murilla, T. 2001. Histology of the oocytes of Lutjanus peru (Nichols and Murphy, 1922) (Pisces: Lutjanidae). Ciencias Marinus 27: 335–349.

    Google Scholar 

  • Luna, L.G. 1992. Histopathological methods and color atlas of special stains and artifacts. American HistoLabs, Gaithersburg, MD.

    Google Scholar 

  • Lye, C.M., Frid, C.L.J. and Gill, M.E. 1998. Seasonal reproductive health of flounder Platichthys flesus exposed to sewage effluent. Mar. Ecol. Prog. Ser. 170: 249–260.

    Google Scholar 

  • Mackay, N.J. 1973. Reproductive cycle of the fire-tail gudgeon, Hypseleotris galii. 2. Seasonal histological changes in the testis. Austr. J. Zool. 21: 53–66.

    Article  CAS  Google Scholar 

  • McCormick, J.H., Stokes, G.N. and Hermanutz, R.O. 1989. Oocyte atresia and reproductive success in fathead minnows (Pimephales promelas) exposed to acidified hardwater environments. Arch. Environ. Contam. Toxicol. 18: 207–214.

    Article  PubMed  CAS  Google Scholar 

  • McMaster, M.E., Van Der Kraak, G.J. and Munkittrick, K.R. 1996. An epidemiological evaluation of the biochemical basis for steroid hormone depressions in fish exposed to industrial wastes. J. Great Lakes Res. 22: 153–171.

    Article  CAS  Google Scholar 

  • Mikaelian, I., de Lafontaine, Y., Harshbarger, J.C., Lee, L.L.J. and Martineau, D. 2002. Health of lake whitefish (Coregonus clupeaformis) with elevated tissue levels of environmental contaminants. Environ. Toxicol. Chem. 21: 532–541.

    Article  PubMed  CAS  Google Scholar 

  • Miles-Richardson, S.R., Kramer, V.J., Fitzgerald, S.D., Render, J.A., Yamini, B., Barbee, S.J. and Giesey, J.P. 1999. Effects of waterborne exposure of 17 β-estradiol on secondary sex characteristics and gonads of fathead minnows (Pimephales promelas). Aquat. Toxicol. 47: 129–145.

    Article  CAS  Google Scholar 

  • Miranda, A.C.L., Bazzoli, N., Rizzo, E. and Sato, Y. 1999. Ovarian follicular atresia in two teleost species: a histological and ultrastructural study. Tis. Cell. 31: 480–488.

    Article  CAS  Google Scholar 

  • Monosson, E., Hogson, R.G., Fleming, W.J. and Sullivan, C.V. 1996. Blood plasma levels of sex steroid hormones and vitellogenin in striped bass (Morone saxatilis) exposed to 3,3′,4,4′-tetrachlorobiphenyl (TCB). Bull. Environ. Contam. Toxicol. 56: 782–787.

    Article  PubMed  CAS  Google Scholar 

  • Munro, A.D., Scott, A.P. and Lam, T.J. (eds.). 1990. Reproductive Seasonality in Teleosts: Environmental Influences. CRC Press, Inc., Baton Raton, FL. 254 pp.

    Google Scholar 

  • Mytilineou, C.H. 2000. Histological study of atresia in Pagellus acarne (Pisces: Sparidae). In: Proceedings of the 6th Hellenic Symposium on Oceanography and Fisheries, Vol. 2, pp. 28–33. NCMR Association of Employees, Athens, Greece.

    Google Scholar 

  • Nagahama, Y. 1983. The functional morphology of teleost gonads. In: Fish Physiology, Vol. IXA, pp. 223–264. Edited by W.S. Hoar, D.J. Randall and E.M. Donaldson. Academic Press, Orlando, FL.

    Google Scholar 

  • Niimi, A.J. 1983. Biological and toxicological effects of environmental contaminants in fish and their eggs. Can. J. Fish. Aquat. Sci. 40: 306–312.

    CAS  Google Scholar 

  • Nolan, M., Jobling, S., Brighty, G., Sumpter, J.P. and Tyler, C.R. 2001. A histological description of intersexuality in the roach. J. Fish Biol. 58: 160–176.

    Article  Google Scholar 

  • Okumura, H., Saeki, F., Matsubara, H., Adachi, S. and Tamauchi, K. 2001. Changes in serum vitellogenin levels and immunohistochemical localization of vitellogenin in hepatic cells during ovarian development in the Japanese eel. Fish. Sci. 67: 880–887.

    Article  CAS  Google Scholar 

  • Palmer, E.E., Sorensen, P.W. and Adelman, I.R. 1995. A histological study of seasonal ovarian development in freshwater drum in the Red Lakes, Minnesota. J. Fish Biol. 47: 199–210.

    Article  Google Scholar 

  • Parrott, J.L., Jardine, J.J., Blunt, B.R., McCarthy, L.H., McMaster, M.E., Wood, C.S., Roberts, J. and Carey, J.H. 1999. Comparing biological responses to mill process changes: A study of steroid concentrations in goldfish exposed to effluent and waste streams from a Canadian bleached sulphite mill. Wat. Sci. Tech. 40: 115–121.

    Article  CAS  Google Scholar 

  • Patiño, R., and Sullivan, C.V. 2002. Ovarian follicle growth, maturation, and ovulation in teleost fishes. Fish Physiol. Biochem. 26: 57–70.

    Article  Google Scholar 

  • Potts, G.W. and Wootton, R.J. (eds). 1984. Fish Reproduction: Strategies and Tactics. Academic Press, New York. 410 pp.

    Google Scholar 

  • Purdom, C.E., Hardiman, P.A., Bye, V.J., Eno, N.C., Tyler, C.R. and Sumpter, J.P. 1994. Estrogenic effects of effluents from sewage treatment works. Chem. Ecol. 8: 275–285.

    CAS  Google Scholar 

  • Ravaglia, M.A. and Maggese, M.C. 1995. Melano-macrophage centers in the gonads of the swamp eel, Synbranchus marmoratus Bloch, (Pisces, Synbranchidae): histological and histochemical characterization. J. Fish Dis. 18: 117–125.

    Article  Google Scholar 

  • Rideout, R.M., Maddock, D.M. and Burton, M.P.M. 1999. Oogenesis and the spawning pattern in Greenland halibut from the North-west Atlantic. J. Fish Biol. 54: 196–207.

    Article  Google Scholar 

  • Rodriguez, J.N., Oteme, Z.J. and Hem, S. 1995. Comparative study of vitellogenesis of two African catfish species Chrysichthys nigrodigitatus (Claroteidae) and Heterobranchus longifilis (Clariidae). Aquat. Living Res. 8: 291–296.

    Google Scholar 

  • Rolland, R.M. 2000. Ecoepidemiology of the effects of pollution on reproduction and survival of early life stages in teleosts. Fish Fish. 1: 41–72.

    Google Scholar 

  • Rolland, R.M., Gilbertson, M. and Petersen, R.E. (eds.). 1997. Chemically induced alterations in functional development and reproduction of fishes. Proceedings from a session at the Wingspread Conference Center, July 1995, Racine, WI. Society of Environmental Toxicology and Chemistry Press, Pensacola, FL. 224 pp.

  • Saidapur, S.K. 1978. Follicular atresia in the ovaries of nonmammalian vertebrates. Int. Rev. Cytol. 54: 225–244.

    Article  PubMed  CAS  Google Scholar 

  • Sangalang, G.B., Freeman, H.C. and Crowell, R. 1981. Testicular abnormalities in cod (Gadus morhua) fed aroclor 1254. Arch. Environ. Contam. Toxicol. 10: 617–626.

    Article  PubMed  CAS  Google Scholar 

  • Santos, J.E., Bazzoli, N., Rizzo, E. and Santos, G.B. 2001. Morphofunctional organization of the male reproductive system, of the catfish Iheringichthys labrosus (Lütken, 1874) (Siluriformes: Pimelodidae). Tis. Cell 33: 533–540.

    Article  CAS  Google Scholar 

  • Schmitt, C.J., Blazer, V.S., Dethloff, G.M., Tillitt, D.E., Gross, T.S., Bryant, W.L. Jr., DeWeese, L.R., Smith, S.B., Goede, R.W., Bartish, T.M. and Kubiak, T.J. 1999. Biomonitoring of Environmental Status and Trends (BEST) Program: Field procedures for assessing the exposure of fish to environmental contaminants. U.S. Geological Survey, Biological Resources Division, Columbia, (MO). Information and Technology Report USGS/BRD-1999–0007, 70 pp.

    Google Scholar 

  • Schmitt, C.J. and Dethloff, G.M. (eds). 2000. Biomonitoring of Environmental Status and Trends (BEST) Program: Selected methods for monitoring chemical contaminants and their effects in aquatic ecosystems. U.S. Geological Survey, Biological Resources Division, Columbia (MO): Information and Technology Report USGS/BRD-2000–0005, 81 pp.

    Google Scholar 

  • Schulz, R.W. and Miura, T. 2002. Spermatogenesis and its endocrine regulation. Fish Physiol. Biochem. 26: 43–56.

    Article  CAS  Google Scholar 

  • Selman, K. and Wallace, R.A. 1989. Cellular aspects of oocyte growth in teleosts. Zool. Sci. 6: 211–231.

    Google Scholar 

  • Selman, K., Wallace, R.A., Sarka, A. and Xiaoping, Q. 1993. Stages of oocyte development in the zebrafish, Brachydanio rerio. J. Morphol. 218: 203–224.

    Article  Google Scholar 

  • Spies, R.B., Stegeman, J.J., Hinton, D.E. Woodin, B., Smolowitz, R., Okihiro, M. and Shea, D. 1996. Biomarkers of hydrocarbon exposure and sublethal effects in embiotocid fishes from a natural petroleum seep in the Santa Barbara Channel. Aquat. Toxicol. 34: 195–219.

    Article  CAS  Google Scholar 

  • Spitsbergen, J.M., Tsai, H.-W., Reddy, A., Miller, T., Arbogast, D., Hendricks, J.D. and Bailey, G.S. 2000. Neoplasia in zebrafish (Danio rerio) treated with N-methyl-N′-nitro-N-nitrosoguanidine by three exposure routes at different developmental stages. Toxicol. Pathol. 28: 716–725.

    PubMed  CAS  Google Scholar 

  • Stott, G.G., McArthur, N.H., Tarpley, R., Jacobs, V. and Sis, R.F. 1981. Histopathologic survey of ovaries of fish from petroleum production and control sites in the Gulf of Mexico. J. Fish Biol. 18: 261–269.

    Article  Google Scholar 

  • Stott, G.G., Haensly, W., Neff, J. and Sharp, J. 1983. Histopathologic survey of ovaries of plaice, Pleuronectes platessa L., from AberWrac'h and Aber Benoit, Brittany, France oil spills. J. Fish. Dis. 6: 429–437.

    Article  Google Scholar 

  • Strüssmann, C.A. and Nakamura, M. 2002. Morphology, endocrinology, and environmental modulation of gonadal sex differentiation in teleost fishes. Fish Physiol. Biochem. 26: 13–29.

    Article  Google Scholar 

  • Sulistyo, I., Fontaine, P., Rinchard, J., Gardeur, J.-N., Migaud, H., Capeville, B. and Kestemont, P. 2000. Reproductive cycle and plasma levels of steroids in male Eurasian perch Perca fluviatilis. Aquat. Living Res. 13: 99–106.

    Article  Google Scholar 

  • Sumpter, J.P. 1995. Feminized responses in fish to environmental estrogens. Toxicol. Lett. 82/83: 737–742.

    Article  PubMed  Google Scholar 

  • Teh, S.J., Adams, S.M. and Hinton, D.E. 1997. Histopathological biomarkers in feral freshwater fish populations exposed to different types of contaminant stress. Aquat. Toxicol. 37: 51–70.

    Article  CAS  Google Scholar 

  • Treasurer, J.W. and Holliday, F.G.T. 1981. Some aspects of the reproductive biology of perch Perca fluviatilis L. A histological description of the reproductive cycle. J. Fish Biol. 18: 359–376.

    Article  Google Scholar 

  • Tyler, C.R. and Sumpter, J.P. 1996. Oocyte growth and development in teleosts. Rev. Fish Biol. Fish. 6: 287–318.

    Article  Google Scholar 

  • Tyler, C.R., Sumpter, J.P. and Witthames, P.R. 1990. The dynamics of oocyte growth during vitellogenesis in the rainbow trout (Oncorhychus mykiss). Biol. Reprod. 43: 202–209.

    Article  PubMed  CAS  Google Scholar 

  • Van Den Belt, K., Wester, P.W., Van Der Ven, L.T.M., Verheyen, R. and Witters, H. 2002. Effects of ethynylestradiol on the reproductive physiology in zebrafish (Danio rerio): time dependency and reversibility. Environ. Toxicol. Chem. 21: 767–775.

    Article  PubMed  CAS  Google Scholar 

  • Wallace, R.A. and Selman, K. 1981. Cellular and dynamic aspects of oocyte growth in teleosts. Amer. Zool. 21: 325–343.

    Google Scholar 

  • Wester, P.W. 1991. Histopathological effects of environmental pollutants β-HCH and methyl mercury on reproductive organs in freshwater fish. Comp. Biochem Physiol. 100C: 237–239.

    CAS  Google Scholar 

  • Wolke, R.E. 1992. Piscine macrophage aggregates: A review. Ann. Rev. Fish Dis. 2: 91–108.

    Article  Google Scholar 

  • Wood, A.W. and Van Der Kraak, G. 2001. Apoptosis and ovarian function: Novel perspectives from the teleosts. Biol. Reprod. 64: 264–271.

    Article  PubMed  CAS  Google Scholar 

  • Yamamoto, K. and Yamazaki, F. 1961. Rhythm of development in the oocyte of the gold-fish, Carassius auratus. Bull. Fac. Fish. Hokkaido Univ. 12: 93–114.

    Google Scholar 

  • Yin, D. 1996. Biochemical basis of lipofuscin, ceroid, and age pigment-like fluorophores. Free Rad. Biol. Med. 21: 871–888.

    Article  PubMed  CAS  Google Scholar 

  • Ziegenfuss, M.C. and Wolke, R.E. 1991. The use of fluorescent microspheres in the study of piscine macrophage aggregate kinetics. Dev. Comp. Immunol. 15: 165–171.

    Article  Google Scholar 

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Blazer, V. Histopathological assessment of gonadal tissue in wild fishes. Fish Physiology and Biochemistry 26, 85–101 (2002). https://doi.org/10.1023/A:1023332216713

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  • DOI: https://doi.org/10.1023/A:1023332216713

  • fish gonads
  • histology
  • ovary
  • pathology
  • testes