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Temporal and Spatial Expression of Fibroblast Growth Factor Receptor 4 Isoforms in Murine Tissues

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Abstract

Fibroblast growth factor receptors (FGFRs) undergo highly regulated spatial and temporal changes of expression during development. This study describes the use of quantitative reverse transcriptase-polymerase chain reaction and immunochemistry to assess the changes in expression of FGFR4 as compared to its FGFR4-17a and -17b isoforms in mouse tissues, from early embryogenesis through to adulthood. Compared to FGFR4, the expression of the isoforms is more restricted at all developmental stages tested. The reverse transcriptase-polymerase chain reaction demonstrated that FGFR4 is expressed in more tissue types than either of its isoforms: it was found predominantly in lung, liver, brain, skeletal muscle and kidney, whereas the FGFR4-17a form was detected in lung and skeletal muscle, and the FGFR4-17b form only in lung, liver, skeletal muscle and kidney. Immunohistochemistry confirmed strong FGFR4-17b expression in the postnatal lung. When combined, the results suggest that FGFR4 variants play important roles particularly in lung and skeletal muscle development.

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Authors and Affiliations

  1. School of Biomedical Sciences, University of Queensland, Brisbane, QLD, 4072, Australia

    Simon M. Cool, Rosamond E. Sayer, Walter R. van Heumen, James O. Pickles & Victor Nurcombe

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  1. Simon M. Cool
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  2. Rosamond E. Sayer
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  3. Walter R. van Heumen
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  4. James O. Pickles
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  5. Victor Nurcombe
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Cool, S.M., Sayer, R.E., van Heumen, W.R. et al. Temporal and Spatial Expression of Fibroblast Growth Factor Receptor 4 Isoforms in Murine Tissues. Histochem J 34, 291–297 (2002). https://doi.org/10.1023/A:1023326524562

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