Abstract
The effect of osmotic shock on the expression of genes in the lux regulon of marine bacteria Vibrio fischeri was studied in cells ofEscherichia coli. Bioluminescence of cells was shown to drastically increase, when cells were exposed to osmotic shock at the early logarithmic growth phase, at far lower optic densities as compared to the critical optic density characteristic. The expression of lux genes induced by osmotic shock is determined by the two-component regulatory system RcsC–RcsB. A nucleotide sequence in the regulatory region of the luxR gene homologous to the RcsB-box consensus of E. coli is assumed to be a primary site for this system.
Similar content being viewed by others
References
Fuqua, W.C., Winans, S.C., and Greenberg, E.P., Quorum Sensing in Bacteria: The LuxR–LuxI Family of Cell Density-Responsive Transcriptional Regulators, J. Bacteriol., 1994, vol. 176, pp. 269–275.
Ulitzur, S. and Dunlop, P., Regulatory Circuitry Controlling Luminescence Autoinduction in Vibrio fischeri, Photochem. Photobiol., 1995, vol. 62, pp. 625–632.
Fuqua, W.C., Winans, S.C., and Greenberg, E.P., Census and Consensus in Bacterial Ecosystems: The LuxR–LuxI Family of Quorum-Sensing Transcriptional Regulators, Annu. Rev. Microbiol., 1996, vol. 50, pp. 727–751.
Gray, K.M. and Garey, J.R., The Evolution of Bacterial LuxI and LuxR Quorum Sensing Regulators, Microbiology, 2001, vol. 147, pp. 2379–2387.
Zavil'gel'skii, G.B. and Manukhov, I.V., Quorum Sensing, or How Bacteria “Talk” to Each Other, Mol. Biol. (Moscow), 2000, vol. 35, pp. 268–277.
Meighen, E.A., Molecular Biology of Bacterial Bioluminescence, Microbiol. Rev., 1991, vol. 55, pp. 123–142.
Tu, S.-C. and Mager, H.I.X., Biochemistry of Bacterial Bioluminescence, Photochem. Photobiol., 1995, vol. 62, pp. 615–624.
Engebrecht, J., Nealson, K.H., and Silverman, M., Bacterial Bioluminescence: Isolation and Genetic Analysis of Functions from Vibrio fischeri, Cell (Cambridge, Mass.), 1983, vol. 32, pp. 773–781.
Eberhard, A., Burlingame, A.L., Eberhard, C., et al., Structural Identification of Autoinducer of Photobacterium fischeri Luciferase, Biochemistry, 1981, vol. 20, pp. 22 444–22 449.
Egland, K.A. and Greenberg, E.P., Quorum Sensing in Vibrio fischeri: Elements of the luxI Promoter, Mol. Microbiol., 1999, vol. 31, pp. 1197–1204.
Engebrecht, J. and Silverman, M., Identification of Genes and Gene Products Necessary for Bacterial Bioluminescence, Proc. Natl. Acad. Sci. USA, 1984, vol. 81, pp. 4154–4158.
Zavil'gel'skii, G.B. and Manukhov, I.V., Lon Protease Participates in Regulating Transcription of the Vibrio fischeri lux Operon, Genetika (Moscow), 1994, vol. 30, no. 3, pp. 337–341.
Miyamoto, C.M., Lin, Y.H., and Meighen, E.A., Control of Bioluminescence in Vibrio fischeri by the LuxO Signal Response Regulator, Mol. Microbiol., 2000, vol. 38, pp. 594–607.
Manukhov, I.V., Rastorguev, S.M., Eroshnikov, G.E., et al., Cloning and Expression of the lux Operon of Photorhabdus luminescens Strain Zm1: Nucleotide Sequence of the luxAB Genes and Main Characteristics of Luciferase, Genetika (Moscow), 2000, vol. 36, no. 3, pp. 322–330.
Miller, J., Experiments in Molecular Genetics, Cold Spring Harbor, New York: Cold Springs Harbor Lab., 1976.
Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1982.
Slout, V. and Gottesman, S., RcsB and RcsC: A Two-Component Regulator of Capsule Synthesis in Escherichia coli, J. Bacteriol., 1990, vol. 172, pp. 659–669.
Carballes, F., Bertrand, C., Bouche, J.P., and Cam, K., Regulation of Escherichia coli Cell Division Genes ftsA and ftsZ by the Two-Component System rcsC–rcsB, Mol. Microbiol., 1999, vol. 34, pp. 442–450.
Takeda, S.-I., Fujisawa, Y., Matsubara, M., et al., A Novel Feature of the Multistep Phosphorelay in Escherichia coli: A Revised Model of the RcsC–YojN–RcsB Signaling Pathway Implicated in Capsular Synthesis and Swarming Behavior, Mol. Microbiol., 2001, vol. 40, pp. 440–450.
Sledjeski, D.D. and Gottesman, S., Osmotic Shock Induction of Capsule Synthesis in Escherichia coli K-12, J. Bacteriol., 1996, vol. 178, pp. 1204–1206.
Ulitzur, S., Involvement of Host Factors in Regulating the lux System of Vibrio fischeri, Microbial Ecology and Infectious Disease, Rosenberg, E., Ed., Washington: Am. Soc. Microbiol., 1999, pp. 123–132.
Wehland, M. and Bernhard, F., The RcsAB Box, J. Biol. Chem., 2000, vol. 275, pp. 7013–7020.
Davalos-Garcia, M., Conter, A., Toesca, I., et al., Regulation of osmC Gene Expression by the Two-Component System rcsB–rcsC in Escherichia coli, J. Bacteriol., 2001, vol. 183, pp. 5870–5876.
Dunlop, P.V. and Greenberg, E.P., Control of Vibrio fischeri Luminescence Gene Expression in Escherichia coli by Cyclic AMP and Cyclic AMP Receptor Protein, J. Bacteriol., 1985, vol. 164, pp. 45–50.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zavil'gel'skii, G.B., Kotova, V.Y. Osmotic Shock Induction of the Expression of Vibrio fischeri lux Genes in Escherichia coli Cells. Russian Journal of Genetics 39, 390–394 (2003). https://doi.org/10.1023/A:1023301529636
Issue Date:
DOI: https://doi.org/10.1023/A:1023301529636