Abstract
Acid phosphatase activity inhibited by 1 mM sodium molybdate was detected at the surface of barley seedling roots and in the cell wall fraction isolated from barley and maize seedling roots. This enzyme hydrolyzed NPP, GP, and PPi at low pH (4.0 and below). NPP hydrolysis was stimulated by magnesium (but not calcium or manganese) ions, while PPi hydrolysis was independent of the presence of bivalent ions. The activity of phosphatase localized in the cell walls of the both crops increased in the presence of 100 μM AlCl3 or CuCl2. Stimulation of NPP hydrolysis by micromolar concentrations of aluminium and copper as well as by millimolar concentrations of magnesium decreased in the presence of 25 μM cAMP. This agrees with the previous data on the enzyme localized at the outer side of the properly oriented vesicles in the microsomal fraction of plasmalemma. The role of the root extracellular acid phosphatase loosely associated with various apoplast structures in plant adaptation to toxic effect of aluminium in the acidic soils as well as possible control of this process by cAMP secretion to the apoplast are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Brown, E.G. and Newton, R.P., Cyclic AMP and Higher Plants, Phytochem., 1981, vol. 720, no.11, pp. 2453–2463.
Crasnier, M., Noat, G., and Ricard, J., Purification and Properties of Acid Phosphatase from Sycamore Cell Walls, Plant Cell Environ., 1980, no. 3, pp. 217–224.
Duff, S.M.G., Sarath, G., and Plaxton, W.C., The Role of Acid Phosphatase in Plant Phosphorus Metabolism, Physiol. Plant., 1994, vol. 90, no.4, pp. 791–812.
Fedorovskaya, M.D., Tikhaya, N.I., Stekhanova, T.N., Znamenskaya, E.R., and Vakhmistrov, D.B., Ecto-Ca-ATPase (Ectonucleotidase) from Barley Root Cells. 1. Soluble and Membrane-Bound Forms, Fiziol. Rast., 1993, vol. 40, no.2, pp. 163–168.
Horst, W.J., The Role of Apoplast in Aluminium Toxicity and Resistance of Higher Plants, Zeit. Pflanzener. Boden., 1995, vol. 158, no.5, pp. 419–428.
Kaneko, T.S., Kikuti, R., and Kubota, K., Purification and Properties of Native Cell Wall Acid Phosphatase from Cultured Tobacco Cells, Phytochem., 1990, vol. 29, no.9, pp. 2883–2887.
Kulaev, I.S., Biokhimiya vysokomolekulyarnykh polifosfatov (Biochemistry of high molecular polyphosphates), Moscow: Mosk. Gos. Univ., 1975.
Kurniatum, H., Aluminium Tolerance of Mucuna, A Tropical Leguminous Cover Crop, Proefschrift Gronninngen. Met Lit. Opg. Met Samenvatting in Het Nederlands, 1992.
Larsen, P.B., Degenhardt, J., Tai Chin-Yin, Stenzler, L.M., Howell, S.H., and Kochian, L.V., Aluminium-Resistant Arabidopsis Mutants that Exhibit Altered Patterns of Aluminium Accumulation and Organic Acid Release from Roots, Plant Physiol., 1998, vol. 117, pp. 9–18.
Lindberg, S. and Griffiths, G., Aluminium Effects on ATPase Activity and Lipid Composition of Plasma Membranes in Sugar Beet Roots, J. Exp. Bot., 1993, vol. 44, no.267, pp. 1543–1550.
Lindeman, N., Observation of the Behavior of Phosphate Compounds in Chlorella at the Transition from Dark to Light, Proc. II Intern. Conf. U.N. on the Peaceful Uses of Atomic Energy, 1985, vol. 24, pp. 8–9.
Marienfeld, S., Lehmann, H., and Stelzer, R., Ultrastructural Investigation and EDX-Analysis of Al-Treated Oat (Avena sativa) Roots, Plant Soil, 1995, vol. 171, no.1, pp. 167–173.
Orlov, S.N. and Maksimova, N.V., cAMP Excretion by Cells: Mechanism and Physiological Role, Biokhimiya, 1999, vol. 64, no.2, pp. 164–173.
Panara, F., Pasgualini, S., and Antonielli, M., Multiple Forms of Barley Root Acid Phosphatase: Purification and Some Characteristics of the Major Cytoplasmic Isoenzyme, Biochem. Biophys. Acta, 1990, vol. 1037, pp. 73–80.
Papernik, L.A. and Kochian, L.V., Possible Involvement of Al-Induced Electrical Signals in Al Tolerance in Wheat, Plant Physiol., 1997, vol. 115, pp. 657–667.
Ratner, E.I., Pitanie rastenii i zhiznedeyatel’nost’ ikh kornevykh sistem (Plant nutrition and physiological activity of their root systems), Moscow: Akad. Nauk SSSR, 1958.
Semenov, I.L. and Vyskrebentseva, E.I., Study of Degradation Processes and ATP Absorption by Sugar Beet Tissues, Fiziol. Rast., 1978, vol. 25, no.5, pp. 1077–1085.
Seregin, I.V. and Ivanov, V.B., Physiological Aspects of Toxic Effect of Cadmium and Lead to Higher Plants (A Review), Fiziol. Rast., 2001, vol. 48, no.4, pp. 606–630.
Shono, N.I. and Baskaeva, E.M., Protein Determination by the Method of Bradford: the Limits of Usability, Lab. Delo, 1989, no. 4, pp. 4–7.
Sivaguru, M., Fujiwara, T., Samaj, J., Baluska, F., Yang, Z., Osawa, H., Maeda, T., Mori, T., Volkmann, D., and Matsumoto, H., Aluminium-Induced 1-3- -Glucan Inhibits Cell-to-Cell Trafficking of Molecules through Plasmodesmata. A New Mechanism of Aluminium Toxicity in Plants, Plant Physiol., 2000, vol. 124, pp. 991–1005.
Thiebartfassy, I. and Hervagault, J.F., Combined Effects of Diffusional Hindrances, Electrostatic Repulsion, and Product Inhibition on the Kinetic-Properties of a Bound Acid Phosphatase, FEBS Lett., 1993, vol. 334, no.1, pp. 89–94.
Tikhaya, N.I. and Fedorovskaya, M.D., Biochemical Adaptation of Barley Root Cells to Toxic Compounds. 1. Effect of Aluminium on Phosphohydrolase Activity, Izv. Ross. Akad. Nauk, Ser. Biol., 2000, no. 5, pp. 569–574.
Tikhaya, N.I. and Fedorovskaya, M.D. Effect of cAMP on Phosphohydrolase Activity of Barley Root Cell Apoplast, Fiziol. Rast., 2002, vol. 49, no.3, pp. 359–364.
Tikhaya, N.I., Fedorovskaya, M.D., and Stekhanova, T.N., Ecto-Ca-ATPase (Ectonucleotidase) from Barley Root Cells. 2. Enzyme Purification and Some Properties, Fiziol. Rast., 1993, vol. 40, no.2, pp. 169–173.
Tikhaya, N.I., Fedorovskaya, M.D., Stekhanova, T.N., and Vakhmistrov, D.B., Ecto-ATPase (Nucleotidase) Activity of Sterile Isolated Roots in Vitro, Fiziol. Rast., 1995, vol. 42, no.6, pp. 821–827.
Tikhaya, N.I., Stekhanova, T.N., and Fedorovskaya, M.D., Is Phosphohydrolase Diversity a Common Feature of the Root Cell Apoplast , Fiziol. Rast., 1999, vol. 46, no.5, pp. 728–737.
Vakhmistrov, D.B., Mishustina, N.E., Ismailov, V.I., Samoilova, S.A., and Tikhaya, N.I., Some Characteristics of Barley Root Surface Phosphatase Activity, Fiziol. Rast., 1973, vol. 20, no.6, pp. 1224–1235.
Verkhovskaya, M.L. and Kurkova, E.B., Isolation of Plasma Membranes from the Cell Wall Fraction and Some Characteristics of ATPase Activity of These Membranes, Fiziol. Rast., 1980, vol. 27, no.6, pp. 1242–1248.
Vyskrebentseva, E.I. and Semenov, I.L., Some Properties of the Surface Phosphatase from Sugar Beet Tissues, Fiziol. Rast., 1976, vol. 23, no.5, pp. 906–913.
Zheng, S.J., Ma, J.F., and Matsumoto, H., High Aluminium Resistance in Buckwheat. 1. Al-Induced Specific Secretion of Oxalic Acid from Root Tips, Plant Physiol., 1998, vol. 117, pp. 745–751.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fedorovskaya, M.D., Tikhaya, N.I. Effect of Aluminium and cAMP on Acid Phosphatase from the Apoplast of Barley and Maize Root Cells. Biology Bulletin 30, 148–154 (2003). https://doi.org/10.1023/A:1023289222270
Issue Date:
DOI: https://doi.org/10.1023/A:1023289222270