Abstract
We studied the effect of naloxone—an antagonist of the opioid receptors—on sensitivity of Cl–-activated Mg2+-ATPase from the plasma membrane fraction of bream brain (Abramis brama L.) to GABAa-ergic substances. Preincubation of the plasma membranes with 1–100 μM naloxone increased the basal Mg2+-ATPase activity and suppressed its activation by chloride ions. The same effects were observed in the presence of the agonists of GABAa/benzodiazepine receptors: 0.1–100 μM GABA, 1–500 μM pentobarbital, and 0.1–100 μM phenazepam. Naloxone (10 μM) inhibited activation of the basal Mg2+-ATPase by the studied ligands and restored the enzyme sensitivity to Cl–. However, the effect of naloxone was not observed in the presence of high concentrations of pentobarbital (500 μM) and phenazepam (100 μM). The obtained data show that naloxone modulates the activity of Cl–-activated Mg2+-ATPase from the plasma membranes of bream brain and antagonizes the GABAa receptor ligands.
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Menzikov, S.A., Menzikova, O.V. Effect of Naloxone on the Activity of Cl–-Activated Mg2+-ATPase from Plasma Membrane Fraction of Bream Brain (Abramis brama L.) in the Presence of GABAa-ergic Substances. Biology Bulletin 30, 119–123 (2003). https://doi.org/10.1023/A:1023281020453
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DOI: https://doi.org/10.1023/A:1023281020453