Abstract
Direct plant regeneration, multiple shoot formation and callogenesis were induced from cocoyam shoot tips cultured in vitro. At different stages of culture, phenol content, peroxidase activity and acidic soluble isoperoxidase patterns were analysed in plantlets. Results showed that phenol content of plantlets cultured on auxin-free media decreased with time, while it increased in those cultured on media supplemented with an auxin. Each form of morphogenesis induced with a growth regulator was preceded by an increase in total peroxidase activity. On hormone-free medium, organogenesis occurred (single shoot development and rhizogenesis), but there was no increase in total peroxidase activity. The appearance of isoperoxidase A2 was associated with root initiation, while the disappearance of isoperoxidase A5 and the appearance of isoperoxidase A6 preceded multiple shoot formation. These results indicate that total peroxidase activity was not a proper marker for organogenesis in cocoyam. Each form of morphogenetic differentiation is associated with an alteration of the acidic isoperoxidase pattern. These enzymes can be used as biochemical markers for rooting and multiple shoot initiation in cocoyam.
Similar content being viewed by others
References
Arnholdt-Schmitt B. 1993. Rapid changes in amplification and methylationpattern of genomic DNA in cultured carrot root explants (Dacus carota L.). Theor. Appl. Genet. 85: 792–800.
Berthon Y., Battraw M.J. and Gaspar T. 1993. Early test usingphenolic compounds and peroxidase activity to improve in vitro rooting of Sequoiadendron giganteum. Saussurea 24: 7–14.
Castillo F.J. 1992. Peroxidase and stress.In: Penel C., Gaspar T.h. and Greppin H. (eds), Plant Peroxidases 1980–1990. Topics and detailed Literature on Molecular, Biochemical and Physiological Aspects. Univ Geneva, Switzerland, pp. 187–203.
Chen L.J. and Luthe D.S. 1987. Analysis ofproteins from embryogenic and non-embryogenic rice (Oryza sativa L.) calli. Plant Sci. 48: 181–188.
Choi J.H. and Sung Z.R. 1984. Two dimensional gel analysis of carrotsomatic embryogenesis proteins. Plant Mol. Biol. Rep. 2: 19–25.
Cui K., Li J., Xing G., Wang L. and Wang Y. 2002. Effect of hydrogen peroxide on synthesis of proteins during somatic embryogenesis in Lycium barbarum. Plant Cell, Tiss. Org. Cult. 68: 187–193.
Davis B.J. 1964. Disc electrophoresis. II. Method and application to human serumproteins. Ann. New York Acad. Sci. 121: 404–427.
De Klerk G.J. 1996.Markers for adventitious root formation. Agronomie 16: 609–616.
Deumling B.and Clermont L. 1989. Changes in DNA content and chromosomal size during culture and plant regeneration of Scilla siberica: selective chromatin diminution in response to environmental conditions. Chromosoma 97: 439–448.
El Hadrami I. and D'Auzac J. 1992.Effects of growth regulators on polyamine content and peroxidase activity in Hevea brasiliensis callus. Ann. Bot. 69: 323–325.
Kay L.E. and Basile D.V. 1987. Specificperoxidase isoenzymes are correlated with organogenesis. Plant Physiol. 84: 99–105.
Klee H. and estelle M. 1990. Molecular genetic approaches to plant hormone biology.Ann. Rev. Plant Physiol. Plant Mol. Biol. 42: 529–551.
Mäder M. and Füssl R.1982. Role of peroxidase in lignification of tobacco cells. II. Regulation by phenolic compounds. Plant Physiol. 70: 1132–1134.
Marigo G. 1973. Sur uneméthode de fractionnement et d'estimation des composés phénoliques chez les végétaux. Analysis 2: 106–110.
Murashige T. and Skoog F. 1962. A revised medium for rapidgrowth and bioassays with tobacco tissue culture. Plant Physiol. 15: 473–497.
Nakajima R. and Yamazaki I. 1979. The mechanism of indole-3-acetic acid oxidation byhorseradish peroxidase. J. Biol. Chem. 254: 872–878.
Omokolo N.D., Tsala N.G., Kanmegne G. and Balangé A.P. 1995. In vitro induction of multiple shoots, plant regeneration and tuberization from shoot tips of cocoyam. C. R. Acad. Sci. 318: 773–778.
Omokolo N.D., Djocgoue P.F., Nana L. and Debost M. 1995. Variation andinheritance of peroxidase activity and phenol and saccharide content in cacao in relation to susceptibility to black pod disease. Biol. Plant 37: 429–436.
Parthier B. 1989.Hormone-induced alterations in plant gene expression. Biochem. Physiol. Pflanz 185: 289–314.
Racchi M.L., Bagnoli F., Balla I. and danti S. 2001. Differential activityof catalase and superoxide dismutase in seedlings and in vitro micropropagated oak (Quercus robur L.). Plant Cell. Rep. 20: 169–174.
Skoog F. and Miller C.O. 1957.Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp. Soc. Exp. Biol. 11: 118–130.
Skriver K. and Mundy J. 1990. Gene expression inresponse to abcissic acid and osmotic stress. Plant Cell 2: 503–512.
Stirn S. and Jacobsen H.J. 1987. Marker proteins for embryogenic differentiation patterns in pea callus. Plant Cell Rep. 6: 50–54.
Svalheim O. and Robertsen B. 1990. Induction ofperoxidases in cucumber hypocotyls by wounding and fungal infection. Physiol. Plant. 78: 261–267.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kanmegne, G., Omokolo, N.D. Changes in phenol content and peroxidase activity during in vitro organogenesis in Xanthosoma sagittifolium L.. Plant Growth Regulation 40, 53–57 (2003). https://doi.org/10.1023/A:1023076629044
Issue Date:
DOI: https://doi.org/10.1023/A:1023076629044