Abstract
Branchlets of broccoli (Brassica oleracea L.) were used to examine ethylene-stimulated chlorophyll catabolism. Branchlets treated with: 1) air (CK); 2) 1 µL·L−1 1-methylcyclopropene (1-MCP) for 14 hr at 20 °C; 3) 1000 µL·L−1 ethylene (C2H4) for 5 hr at 20 °C; or 4) 1-MCP then C2H4, were stored in the dark at 20 °C for up to 3 d. Chlorophyll (Chl) content and branchlet hue angle decreased during the storage period and 1-MCP treatment delayed this change. Chl degradation in broccoli was accelerated by exposure to C2H4, especially for Chl a. Prior treatment with 1-MCP prevented degreening stimulated by C2H4. Lipoxygenase activity was not altered by any of the treatments, however, 1-MCP with or without ethylene resulted in reduced activity of chlorophyllase (Chlase) and peroxidase (POD). Exposure to C2H4 stimulated Chlase activity and extended the duration of high POD activity. Treatment with 1-MCP followed by C2H4 resulted in reduced POD activity and delayed the increase in Chlase activity. The results suggest chlorophyll in broccoli can be degraded via the POD – hydrogen peroxide system. Exposure to C2H4 enhances activity of Chlase and extends the duration of high POD activity, and these responses may accelerate degreening. Treatment with 1-MCP delays yellowing of broccoli, an effect that may be due to the 1-MCP-induced reduction in POD and Chlase activities.
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References
Abe K., Gong Y. and Chachin K. 1995. Effects of O2 and CO2concentrations and ethyl alcohol and acetaldehyde on the yellowing of broccoli (Brassica oleracea L. var. italica Plenck) sections. J. Jpn. Soc. Cold Preservation of Food 21: 205–210.
Abeles F.B., Dunn L.J., Morgens P., Callahan A., Dinterman R.E. and Schmidt J. 1988.Induction of 33-kD and 60-kD peroxidases during ethylene-induced senescence of cucumber cotyledons. Plant Physiol. 87: 609–615.
Amir-Shapira D., Goldschmidt E.E. and Altman A. 1987. Chlorophyll catabolism in senescing plant tissues: In vivo breakdown intermediates suggest different degradative pathways for citrus fruit and parsley leaves. Proc. Natl. Acad. Sci. 84: 1901–1905.
Boyer N. and de Jaegher G. 1986. Direct or indirectrole of peroxidase in ethylene biosynthesis? In: Greppin H., Penel C. and Gaspar Th. (eds), Molecular and Physiological Aspects of Plant Peroxidase. University of Geneva, Geneva, pp. 47–60.
Bradford M.M. 1976. A rapid and sensitive method for the quantitation ofmicrogram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Gahagan H.E., Holm R.E. and Abeles F.B. 1968. Effect ofethylene on peroxidase activity. Physiol. Plant. Suppl. 21: 1270–1279.
Gong Y.1993. Inhibiting mechanism on discoloration of some vegetables by packaging with polyethylene films. PhD Dissertation, Osaka Prefecture University, Japan.
Fan X. and Mattheis J.P. 2000. Yellowing of broccoli in storage is reduced by 1 methylcyclopropene. HortScience 35: 885–887.
Hildebrand D.F. 1989. Lipoxygenases. PhysiolPlant 76: 249–253.
Holden M. 1961. The breakdown of chlorophyll bychlorophyllase. Biochem. J. 78: 359–364.
Ku H.S., Yang S.F. and Pratt H.K.1970. Ethylene production and peroxidase activity during tomato fruit ripening. Plant Cell Physiol. 11: 241–246.
Ku V.V.V. and Wills R.B.H. 1999. Effect of 1-methylcyclopropene on the storage life of broccoli. Postharvest Biol. Technol. 17: 127–132.
McGuire R.G. 1992. Reporting of objective color measurements. HortScience27: 1254–1255.
Romero M.V. and Barrett D.M. 1997. Rapid methods forlipoxygenase assay in sweet corn. J. Food Sci. 62: 696–700.
Sabater B. and Rodrisol M.T. 1978. Control of chlorophyll degradation in detached leaves of barley and oat through effect of kinetin on chlorophyllase. Physiol Plant 43: 274–276.
Sisler E.C., Blankenship S.M. and Guest M. 1990. Competition of cyclooctenes and cyclooctadienes for ethylene binding and activity in plants. Plant Growth Reg. 9: 157–164.
Tain M.S., Downs C.G., Lill R.E. and King G.A. 1994. A role for ethylene in the yellowing of broccoli after harvest. J. Amer. Soc. Hort. Sci. 119: 276–281.
Trebitsh T., Goldschmidt E.E.and Riov J. 1993. Ethylene induces de novo synthesis of chlorophyllase, a chlorophyll degrading enzyme, in Citrus fruit peel. Proc. Natl. Sci. USA 90: 9441–9445.
Vetter J.L., Steinberg M.P. and Nelson A.I. 1958. Quantitative determination ofperoxidase in sweet corn. Agr. Food Chem. 6: 39–41.
Yamauchi N. and Minamide T. 1985. Chlorophyll degradation by peroxidase in parsley leaves. J. Jpn. Soc. Hort. Sci. 54: 265–271.
Yamauchi N., Iida S., Minamide T. and Iwada T. 1986.Polar lipids content and their fatty acid composition with reference to yellowing of stored spinach leaves. J. Jpn. Soc. Hort. Sci. 55: 355–362.
Yamauchi N., Iida S., Minamide T. and Iwada T. 1987. Localization of lipolytic acyyl hydrolase in chloroplast of stored spinach leaves. J. Jpn. Soc. Hort. Sci. 55: 510–515.
Yamauchi N. and Watada A.E.1991. Regulated chlorophyll degradation in spinach leaves during storage. J. Amer. Soc. Hort. Sci. 116: 58–62.
Zhuang H., Hildebrand D.F. and Barth M.M. 1995.Senescence of broccoli buds is related to changes in lipid peroxidation. J. Agric. Food Chem. 43: 2585–2591.
Wang C.Y. 1977. Effect of amimoethyoxy analog ofrhizobitoxine and sodium benzoate on senescence of broccoli. HortScience 12: 54–56.
MacKinney G. 1941. Absorption of light by chlorophyll solutions. J. Biol. Chem.140: 315–322.
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Gong, Y., Mattheis, J.P. Effect of ethylene and 1-methylcyclopropene on chlorophyll catabolism of broccoli florets. Plant Growth Regulation 40, 33–38 (2003). https://doi.org/10.1023/A:1023058003002
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DOI: https://doi.org/10.1023/A:1023058003002