Abstract
Wheatgrasses (Thinopyrum, Agropyron and Pascopyrum spp.) are a ubiquitous group of cool-season grasses used throughout much of the semiarid temperate world. In order to explore the potential of biotechnology to accelerate conventional breeding efforts, we developed an efficient plant regeneration system for different wheatgrass species: tall wheatgrass [Thinopyrum ponticum (Podp.) Liu and Wang], intermediate and pubescent wheatgrass [Thinopyrum intermedium (Host) Barkw. and D.R. Dewey], crested wheatgrass [Agropyron cristatum (L.) Gaertner], and western wheatgrass [Pascopyrum smithii (Rydb.) Löve]. Embryogenic callus was induced from mature and immature embryos and immature inflorescence with an induction frequency in the range of 0.5–8.3% for the different wheatgrass species tested. Individual embryogenic calluses were then used to establish single genotype-derived suspension cultures. Efficient plant regeneration was achieved from the established em-bryogenic suspensions with regeneration frequencies in the range of 20–65% for tall wheatgrass, 21–40% for intermediate and pubescent wheatgrasses, 32–51% for crested wheatgrass, and 25–48% for western wheatgrass. The cell suspension-derived wheatgrass plants were fertile and phenotypically normal in the field. The efficient plant regeneration system provides a solid basis for genetic transformation of wheatgrasses.
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References
Asay KH (1995) Wheatgrasses and wildryes: the perennial Triticeae. In: Barns RF, Miller DA & Nelson CJ (eds) Forages: An Introduction to Grassland AgricultureVol. 1 (pp. 373-394). Iowa State University Press, Ames, Iowa
Asay KH & Jensen KB (1996) Wheatgrasses. In: Moser LE, Buxton DR & Casler MD (eds) Cool-Season Forage Grasses (pp. 691-724). Agron. Monogr. No. 34, ASA-CSSA-SSSA, Madison, Wisconsin
Brede D (2000) Turf Grass Maintenance Reduction Handbook: Sports, Lawns and Golf. In: Turf Grass Maintenance Reduction Handbook: Sports, Lawns and Golf (pp. 99-101). Ann Arbor Press, Chelsea, Michigan
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY & Bi FY (1975) Establishment of an efficient medium for anther culture of rice, through comparative experiments on the nitrogen sources. Scientia Sinica 18: 659-668
Dewey DR (1962) The genomic structure of intermediate wheat-grass. J. Hered. 53: 282-290
Dewey DR (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP (ed) Gene Manipulation in Plant Improvement (pp. 209-279). Plenum, New York
Jensen KB, Zhang YF & Dewey DR (1990) Mode of pollination of perennial species of the Triticeae in relation to genomically defined genera. Can. J. Plant Sci. 70: 215-225
Lo PF, Chen CH & Ross JG (1980) Vegetative propagation of temperate forage grasses through callus culture. Crop Sci. 20: 363-367
Malakouti MJ, Lewis DT & Stubbendieck J (1978) Effect of grasses and soil properties on wind erosion in sand blowouts. J. Range Manage 31: 417-420
Marburger JE & Wang RRC (1988) Anther culture of some perennial triticeae. Plant Cell Rep. 7: 313-317
Moore KJ, Vogel KP, Klopfenstein TJ, Masters RA & Anderson BE (1995) Evaluation of four intermediate wheatgrass populations under grazing. Agron. J. 87: 744-747
Müller AJ & Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol. Gen.Genet. 161: 67-76
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15: 473-497
Potrykus I (1991) Gene transfer to plants: assessment of published approaches and results. Annu. Rev. Plant Physiol. Plant Mol.Biol. 42: 205-225
Redway FA, Vasil V, Lu D & Vasil IK (1990) Identification of callus types for long-term maintenance and regeneration from commercial cultivars of wheat (Triticum aestivum L.). Plant Cell Rep. 79: 609-617
Shi D, Xi T & Zhu T (1989) Somatic embryogenesis from mature embryo of tall wheatgrass (Agropyron elongatum) and effects of NaCl on induction and growth of callus from its different explants. Proceedings of the XVI International Grassland Congress, 4-11 October 1989, Nice, France (pp. 443-444)
Spangenberg G, Wang ZY, Wu XL, Nagel J, Iglesias VA & Potrykus I (1995) Transgenic perennial ryegrass (Lolium perenne) plants from microprojectile bombardment of embryogenic suspension cells. Plant Sci. 108: 209-217
Spangenberg G, Wang ZY & Potrykus I (1998) Biotechnology in Forage and Turf Grasses Improvement. In: Biotechnology in Forage and Turf Grasses Improvement (pp. 1-5). Springer-Verlag, Berlin, Heidelberg, New York
Vogel KP & Moore KJ (1998) Forage yield and quality of tall wheatgrass accessions in the USDA germplasm collection. Crop Sci. 38: 509-512
Wang ZY, Hopkins A & Mian R (2001) Forage and turf grass biotechnology. Crit. Rev. Plant Sci. 20: 573-619
Wang ZY, Nagel J, Potrykus I & Spangenberg G (1993) Plants from cell suspension-derived protoplasts in Lolium species. Plant Sci. 94: 179-193
Yao K, Chen CH & McMullen CR (1991) Regeneration of albino shoots in cultured anthers of intermediate wheatgrass (Agropyron intermedium (Host) Beauv.). Plant Cell Tiss. Org. Cult. 26: 189-193
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Wang, Z., Bell, J. & Hopkins, A. Establishment of a plant regeneration system for wheatgrasses (Thinopyrum, Agropyron and Pascopyrum). Plant Cell, Tissue and Organ Culture 73, 265–273 (2003). https://doi.org/10.1023/A:1023044913155
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DOI: https://doi.org/10.1023/A:1023044913155