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Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosum

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Abstract

Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytuberol) detected in elicitor-treated hairy root cultures, rishitin (213 μg g−1 dry wt) was the most predominant followed by lubimin (171 μg g−1 dry wt). The elicitors also induced LOX activity (25-fold increase) and LOX metabolites, mainly 9-hydroxyoctadecadienoic acid and 9-hydroxyoctadecatrienoic acid, in potato hairy root cultures. The combination of fungal elicitor plus cyclodextrin was the most effective elicitor treatment, followed by methyl jasmonate plus cyclodextrin in inducing sesquiterpenes and LOX metabolites.

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Authors and Affiliations

  1. School of Life Sciences, University of Hyderabad, Hyderabad, 500 046, India

    P. Komaraiah, G.V. Reddy, P. Srinivas Reddy, A.S. Raghavendra & P. Reddanna

  2. Reliance Life Sciences Pvt. Ltd., Mumbai, 400 033, India

    S.V. Ramakrishna

Authors
  1. P. Komaraiah
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  2. G.V. Reddy
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  3. P. Srinivas Reddy
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  4. A.S. Raghavendra
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  5. S.V. Ramakrishna
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  6. P. Reddanna
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Komaraiah, P., Reddy, G., Reddy, P.S. et al. Enhanced production of antimicrobial sesquiterpenes and lipoxygenase metabolites in elicitor-treated hairy root cultures of Solanum tuberosum . Biotechnology Letters 25, 593–597 (2003). https://doi.org/10.1023/A:1023038804556

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  • DOI: https://doi.org/10.1023/A:1023038804556

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