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Recovery of the proteose peptone component 3 from cheese whey in Reppal PES 100/polyethylene glycol aqueous two-phase systems

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Abstract

Recovery of the proteose peptone component 3 from cheese whey was optimal using a 16% (w/w) Reppal PES 100 – 24% (w/w) PEG 600 aqueous two-phase system, at pH 7, giving a mass recovery yield of 99% and a purity of 83% for proteose peptone component 3 in the upper phase. Using the above system a partition coefficient of 30.7 and a purification factor of 6.9 were achieved.

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Authors and Affiliations

  1. Centro de Engenharia Biológica – IBQF, Universidade do Minho, Largo do Paço, 4719, Braga codex, Portugal

    L.R. Rodrigues, A. Venâncio & J.A. Teixeira

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  1. L.R. Rodrigues
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  2. A. Venâncio
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  3. J.A. Teixeira
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Rodrigues, L., Venâncio, A. & Teixeira, J. Recovery of the proteose peptone component 3 from cheese whey in Reppal PES 100/polyethylene glycol aqueous two-phase systems. Biotechnology Letters 25, 651–655 (2003). https://doi.org/10.1023/A:1023020316445

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  • DOI: https://doi.org/10.1023/A:1023020316445

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