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Lipase-catalyzed synthesis of monoacylglycerol in a homogeneous system

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Abstract

The 1,3-regiospecifique lipase, Lipozyme IM, catalyzed the esterification of lauric acid and glycerol in a homogeneous system. To overcome the drawback of the insolubility of glycerol in hexane, which is extensively used in enzymatic synthesis, a mixture of n-hexane/tert-butanol (1:1, v/v) was used leading to a monophasic system. The conversion of lauric acid into monolaurin was 65% in 8 h, when a molar ratio of glycerol to fatty acid (5:1) was used with the fatty acid at 0.1 M, and the phenomenon of acyl migration was minimized.

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Authors and Affiliations

  1. Departamento de Engenharia Química e Engenharia de Alimentos, Brazil

    Julieta B. Monteiro

  2. Departamento de Química, Universidade Federal de Santa Catarina, CEP 88040-900, Trindade, Florianópolis, Santa Catarina, Brazil

    Julieta B. Monteiro & Maria G. Nascimento

  3. Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, CEP 88040-900, Trindade, Florianópolis, Santa Catarina, Brazil

    Jorge L. Ninow

Authors
  1. Julieta B. Monteiro
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  2. Maria G. Nascimento
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  3. Jorge L. Ninow
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Monteiro, J.B., Nascimento, M.G. & Ninow, J.L. Lipase-catalyzed synthesis of monoacylglycerol in a homogeneous system. Biotechnology Letters 25, 641–644 (2003). https://doi.org/10.1023/A:1023016215537

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  • DOI: https://doi.org/10.1023/A:1023016215537

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