Skip to main content
SpringerLink
Log in

Correlation Between Human Follicular Diameter and Oocyte Outcomes in an ICSI Program

  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose: To determine the correlation between the follicular sizes and oocyte recovery, metaphase II oocyte recovery, fertilization rate and good embryo quality from mature and immature oocytes in an intracytoplasmic sperm injection (ICSI) program.

Methods: 991 follicles obtained from 72 ICSI cycles were classified into three groups according to their diameters as measured by transvaginal ultrasound including group A (<10 mm), group B (10–14 mm), and group C (>14 mm). All obtained oocytes were classified according to their nuclear maturation: germinal vesicle (GV), metaphase I (MI) and metaphase II (MII). Mature oocytes underwent ICSI while immature oocytes were further cultured until maturity before ICSI was performed. The rates of fertilization and good quality embryos at day 3 were evaluated.

Results: A progressive and significant increase in the rates of oocyte recovery and MII oocyte recovery were observed from group A follicles compared to the other groups (p < 0.001). The fertilization rate of mature and in vitro matured oocytes, as well as the rate of good quality embryos showed a tendency to increase from group A to group C follicles, but not significantly. The corresponding fertilization rates were 78 and 55.3% (p < 0.001) for mature and in vitro matured oocytes, respectively.

Conclusion: Collection of oocytes from small follicles, especially with a mean diameter less than 10 mm, and in vitro maturation of immature oocytes before fertilization may allow the total number of good quality and transferable embryos to be increased.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wittmaack FM, Tureck RW, Kreger DO, Mastroianni L, Jr, Blasco L, Lessey BA: Effect of follicular size on oocyte retrieval, fertilization, cleavage and embryo quality in in vitro fertilization cycles: A 6-years data collection. Fertil Steril 1994;62:1205–1210

    Google Scholar 

  2. Ectors FJ, Vanderzwalmen P, Hoeck JV, Nijs M, Verhaegen G, Delvigne A, Schoysman R, Leroy F: Relationship of human follicular diameter with oocyte fertilization and development after in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod 1997;12:2002–2005

    Google Scholar 

  3. Tsuji K, Sowa M, Nakano R: Relationship between human oocyte maturation and different follicular sizes. Biol Reprod 1985;32:413–417

    Google Scholar 

  4. Scott RT, Hofmann GE, Muasher SJ, Acosta AA, Kreiner DK, Rosenwaks Z: Correlation of follicular diameter with oocyte recovery and maturity at the time of transvaginal follicular aspiration. J In Vitro Fertil Embryo Transfer 1989;6:73–75

    Google Scholar 

  5. Dubey AK, Wang HA, Duffy P, Penzias AS: The correlation between follicular measurements, oocyte morphology, and fertilization rates in an in vitro fertilization program. Fertil Steril 1995;64:787–790

    Google Scholar 

  6. Van Steirteghem AC, Liu J, Joris H, Nagy ZP, Cecile J, Tournaye H, Derde MP, Van Assche E, Devroey P: Higher success rate by intracytoplasmic sperm injection than subzonal insemination. A report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993;8:1055–1060

    Google Scholar 

  7. Yovich J, Grudzinskas G: The Management of Infertility: A Manual of Gamete Handling Procedures. Oxford, Heinemann Medical, 1990

    Google Scholar 

  8. Blackledge DG, Thomas WP, Turner SR, Richardson PA, Matson PL, Yovich JL: Transvaginal ultrasonically-guided oocyte pick-up. Med J Aust 1986;145:300

    Google Scholar 

  9. Vanderhyden BC, Telfer EE, Eppig JJ: Mouse oocytes promote proliferation of granulosa cells from preantral and antral follicles in vitro. Biol Reprod 1992;46:1196–1204

    Google Scholar 

  10. Suchanek E, Simunic V, Juretic D, Grizelj V: Follicular fluid contents of hyaluronic acid, follicle-stimulating hormone and steroids relative to the success of in vitro fertilization of human oocytes. Fertil Steril 1994;62:347–352

    Google Scholar 

  11. Oktay K, Briggs D, Gosden RG: Ontogeny of follicle stimulating hormone receptor gene expression in isolated human ovarian follicle. J Clin Endocrinol Metab 1997;82:3748–3751

    Google Scholar 

  12. Minegishi T, Tano M, Igarashi M, Rokukawa S, Abe Y, Ibuki Y, Miyamoto K: Expression of follicle-stimulating hormone receptor in human ovary. Eur J Clin Invest 1997;27:469–474

    Google Scholar 

  13. Russel JB: Immature oocyte retrieval combined with in vitro oocyte maturation. Hum Reprod 1998;13(Suppl):63–70

    Google Scholar 

  14. Trounson A, Wood C, Kausche A: In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril 1994;62:353–362

    Google Scholar 

  15. Cha KY, Han SY, Chung HM, Choi DH, Lim JM, Lee WS, Ko JJ, Yoon TK: Pregnancies and deliveries after in vitro maturation culture followed by in vitro fertilization and embryo transfer without stimulation in women with polycystic ovary syndrome. Fertil Steril 2000;73:978–983

    Google Scholar 

  16. Miller KF, Goldberg JM, Falcone T: Follicular size and implantation of embryos from in vitro fertilization. Obstet Gynecol 1996;88:583–586

    Google Scholar 

  17. Bergh C, Broden H, Lundin K, Hamberger L: Comparision of fertilization, cleavage and pregnancy rates of oocytes from large and small follicles. Hum Reprod 1998;13:1912–1915

    Google Scholar 

  18. Nogueira D, Staessen C, Vande Velde H, Van Steirteghem A: Nuclear status and cytogenetics of embryos derived from in vitro-matured oocytes. Fertil Steril 2000;74:295–298

    Google Scholar 

  19. Ludwig M, Finas DF, Al-Hasani S, Diedrich K, Ortmann O: Oocyte quality and treatment outcome in intracytoplasmic sperm injection cycles of polycystic ovarian syndrome patients. Hum Reprod 1999;14:354–358

    Google Scholar 

  20. Nagy ZP, Loccufier A, Cecile J, Devroey P, Liu J, Van Steirteghem A: Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal vesicle stage oocyte: Case report. Fertil Steril 1996;65:1047–1050

    Google Scholar 

  21. Trounson A, Anderiesz C, Jones GM, Kausche A, Lolatgis N, Wood C: Oocyte maturation. Hum Reprod 1998;13(Suppl):52–62

    Google Scholar 

  22. Morgan PM, Warikoo PK, Bavister BD: In vitro maturation of ovarian oocytes from unstimulated rhesus monkeys: Assessment of cytoplasmic maturity by embryonic development after in vitro fertilization. Biol Reprod 1991;45:89–93

    Google Scholar 

  23. Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, Tiglias J, Wood C, Trounson AO: Blastocyst development and birth after in vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod 1995;10:101–105

    Google Scholar 

  24. Trounson AO, Bongso A, Szell A: Maturation of human and bovine primary oocytes in vitro for fertilization and embryo production. Singapore J Obstet Gynecol 1996;27:78–84

    Google Scholar 

  25. Fulka J, First NL, Moor RM: Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation. Mol Hum Reprod 1998;4:41–49

    Google Scholar 

  26. Mikkelsen AL, Smith S, Lindenberg S: Impact of oestradiol and inhibin A concentrations on pregnancy rate in in-vitro oocyte maturation. Hum Reprod 2000;15:1685–1690

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

    Anusorn Triwitayakorn, Somchai Suwajanakorn, Kamthorn Pruksananonda, Wisan Sereepapong & Vichuda Ahnonkitpanit

Authors
  1. Anusorn Triwitayakorn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Somchai Suwajanakorn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kamthorn Pruksananonda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wisan Sereepapong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Vichuda Ahnonkitpanit
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Triwitayakorn, A., Suwajanakorn, S., Pruksananonda, K. et al. Correlation Between Human Follicular Diameter and Oocyte Outcomes in an ICSI Program. J Assist Reprod Genet 20, 143–147 (2003). https://doi.org/10.1023/A:1022977002954

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022977002954

Search

Navigation