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Inhibitory effects of brown algal phlorotannins on secretory phospholipase A2s, lipoxygenases and cyclooxygenases

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Abstract

The inhibitory effects of brown algal phlorotannins on secretory phospholipase A2s (sPLA2s), lipoxygenases (LOXs) and cyclooxygenases (COXs) were determined with an in vitro assay. Oligomers of phloroglucinol; eckol (a trimer), phlorofucofuroeckol A (a pentamer), dieckol (a hexamer) and 8,8′-bieckol (a hexamer) isolated from the brown alga Eisenia bicyclis had pronounced inhibitory effects on sPLA2 from porcine pancreas and bee venom (IC50 100–200 μM). The phlorotannins inhibited LOX activity more effectively than the well-known LOX inhibitors; resveratrol and epigallocatechin gallate. 8,8′-Bieckol, the strongest LOX inhibitor in this study, inhibited soybean LOX and 5-LOX with IC50 values of 38 and 24 μM, respectively. Negligible or very weak effects of the phlorotannins on COX-1 and COX-2 were found, except for an inhibitory effect of dieckol on COX-1 (74.7%) and of eckol on COX-2 (43.2%) at 100 μM.

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Authors and Affiliations

  1. Laboratory of Marine Resource Chemistry, Department of Bioscience and Biotechnology, Graduate School of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Toshiyuki Shibata, Kohki Nagayama, Kuniko Yamaguchi & Takashi Nakamura

  2. Department of Food Science, National University of Fisheries, Yoshimi, Shimonoseki, 759-65, Japan

    Ryusuke Tanaka

Authors
  1. Toshiyuki Shibata
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  2. Kohki Nagayama
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  3. Ryusuke Tanaka
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  4. Kuniko Yamaguchi
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  5. Takashi Nakamura
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Shibata, T., Nagayama, K., Tanaka, R. et al. Inhibitory effects of brown algal phlorotannins on secretory phospholipase A2s, lipoxygenases and cyclooxygenases. Journal of Applied Phycology 15, 61–66 (2003). https://doi.org/10.1023/A:1022972221002

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