Abstract
Acute prenecrotic damage to cardiomyocytes of lytic (myocytolysis, cytolysis) and contracture-lytic (primary lumpy degradation of myofibrils) types during ischemic and metabolic alteration of the myocardium are detected at the photooptic level by means of photochemical fluorochrome staining and examination under fluorescent light. Comparison of the fluorescent and polarization microscopic pictures showed that changes in cardiomyocytes are determined by local mass redistribution in the sarcomere compartments and transformations of birefraction of myofibrillar system components during necrobiosis. These changes are determined by lysis and coagulation processes in protein structures of sarcomeres.
Similar content being viewed by others
REFERENCES
A. G. Kamkin and I. S. Kiseleva, Uspekhi Fiziol. Nauk, 31,No. 2, 51–58 (2000).
N. V. Karsanov, Byull. Eksp. Biol. Med., 128,No. 8, 124–140 (1999).
V. S. Markhasin, V. Ya. Izakov, and V. I. Shumakov, Physiological Bases of Disorders in Myocardial Contractile Function [in Russian], St. Petersburg (1994).
L. M. Nepomnyashchikh, Byull. Eksp. Biol. Med., 121,No. 1, 4–13 (1996).
L. M. Nepomnyashchikh, Alterative Insufficiency of Cardiac Muscle Cells in Metabolic and Ischemic Injuries [in Russian], Moscow (1998).
L. M. Nepomnyashchikh, G. I. Nepomnyashchikh, E. L. Lushnikova, et al., Morphogenesis of the Priority Pathological Processes in Human and Animal Organs and Tissues: 5 Scientific Discoveries in Biology and Medicine [in Russian], Moscow (1998).
L. M. Nepomnyashchikh and V. G. Zimmermann, Byull. Eksp. Biol. Med., 128,No. 9, 356–360 (1999).
L. M. Nepomnyashchikh and V. G. Zimmermann, Ibid., 132,No. 9, 343–349 (2001).
Yu. G. Tsellarius and L. A. Semenova, Histology of Focal Metabolic Myocardial Lesions [in Russian], Novosibirsk (1972).
Yu. G. Tsellarius, L. A. Semenova, and L. M. Nepomnyashchikh, Pathological Diagnosis of Myocardial Prenecrotic Changes and Infarction by Polarization Microscopy [in Russian], Moscow (1979).
Yu. G. Tsellarius, L. A. Semenova, and L. M. Nepomnyashchikh, Arkh. Patol., 42,No. 12, 3–13 (1980).
Yu. G. Tsellarius, V. G. Zimmermann, L. M. Nepomnyashchikh, and L. A. Semenova, Use of Photochemical Fluorochrome Staining for Pathological Diagnosis of Myocardial Prenecrotic Changes and Infarction [in Russian], Moscow (1984).
V. G. Zimmermann and Yu. G. Tsellarius, Arkh. Patol., 38,No. 7, 72–74 (1976).
I. D. Shperling, Ibid., 49,No. 9, 62–67 (1987).
B. N. Carle, Human Pathol., 12,No. 7, 643–647 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zimmermann, V.G., Nepomnyashchikh, L.M. Lytic and Contracture-Lytic Prenecrotic Damage to Cardiomyocytes: Photochemical Fluorochrome Staining and Fluorescent Microscopy of the Myocardium. Bulletin of Experimental Biology and Medicine 134, 571–577 (2002). https://doi.org/10.1023/A:1022969313879
Issue Date:
DOI: https://doi.org/10.1023/A:1022969313879