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HP1/ORC Complex and Heterochromatin Assembly

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Abstract

We have used the highly conserved heterochromatin component, heterochromatin protein 1 (HP1), as a molecular tag for purifying other protein components of Drosophila heterochromatin. A complex of HP1 associated with the origin recognition complex (ORC) and an HP1/ORC-associated protein (HOAP) was purified from the maternally loaded cytoplasm of early Drosophila embryo. We propose that the DNA-binding activities of ORC and HOAP function to recruit underphosphorylated isoforms of HP1 to sites of heterochromatin nucleation. The roles of highly phosphorylated HP1, other DNA-binding proteins known to interact with HP1, and histone modifying activities in heterochromatin assembly are also addressed.

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  1. School of Biological Sciences, University of Kentucky, 101 T.H. Morgan Building, Lexington, KY, 40506, USA

    Mohammed M. Shareef, RamaKrishna Badugu & Rebecca Kellum

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  1. Mohammed M. Shareef
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  2. RamaKrishna Badugu
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Shareef, M.M., Badugu, R. & Kellum, R. HP1/ORC Complex and Heterochromatin Assembly. Genetica 117, 127–134 (2003). https://doi.org/10.1023/A:1022963223220

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