Abstract
Previously, there has not been any in situ conservation sites for crop germplasm within the United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Plant Germplasm System (NPGS). Using morphologic and molecular (SSR markers) techniques, we assessed the genetic variation present in populations of rock grape (Vitis rupestris Scheele), a native American grape species, throughout its range. We identified seven in situ conservation sites for rock grape using a strategy based on morphologic and molecular data, taxonomic information, population size and integrity, and landholder commitment. In collaboration with federal and state landholding agencies, we have established the first NPGS in situ conservation sites for American wild relatives of a crop.
Similar content being viewed by others
References
Chase M.W. and Hills H.H. 1991. Silica gel: An ideal material for field preservation of leaf samples for DNA studies. Taxon 40: 215–220.
Cohen J.I., Williams J.T., Plucknett D.L. and Shands H. 1997. Ex situ conservation of plant genetic resources: global development and environmental concerns. Science 253: 866–872.
CYTEL Software Corp. 1996. StatXact-;3 for Windows. Cambridge
Falconer D.S. 1989. Introduction to Quantitative Genetics. 3rd edn. Longman, New York, N.Y.
Holden J., Peacock J. and Williams T. 1993. Genes, Crops, and the Environment. Cambridge University Press, Cambridge, United Kingdom.
Hoyt E. 1992. Conserving the Wild Relatives of Crops. 2nd edn. Intl. Board for Plant Genetic Resources, Rome;World Conservation Union, and Worldwide Fund for Nature, Gland, Switzerland.
IPGRI, UPOV and OIV. 1997. Descriptors for Grapevine (Vitis spp.). International Union for the Protection of New Varieties of Plants, Geneva, Switzerland /Office International de la Vigne et du Vin, Paris, France/ International Plant Genetic Resources Institute, Rome, Italy.
Lamboy W.F. and Alpha C.G. 1998. Using simple sequence repeats (SSRs) for DNA fingerprinting germplasm accessions of grape (Vitis L.) species. J. Amer. Soc. Hort. Sci. 123: 182–188.
Lin H. and Walker M.A. 1997. Extracting DNA from cambium describtissue for analysis of grape rootstocks. HortScience 32: 1264–1266.
Maxted N., Ford-;Lloyd B.V. and Hawkes J.G. (eds) 1997. Plant Genetic Conservation: The In Situ Approach. Chapman and Hall, New York, N.Y.
Maxted N. and Hawkes J.G. 1997. Selection of target taxa. In: Maxted N., Ford-;Lloyd B.V. and Hawkes J.G. (eds), Plant Genetic Conservation: The In Situ Approach. Chapman and Hall, New York, N.Y. pp. 43-;68.
Merezhko A.F. 1998.In situ and ex situ conservation of plant Conservagenetic diversity are links of a common back-;up system. In: Zencirci N., Kaya Z., Anikster Y. and Adams W.T. (eds), The proceedings of international symposium on in situ conservation of plant genetic diversity, pp. 49–53, Antalya, Turkey, 4–8 Nov. 1996. Central Res. Inst. for Field Crops, Ankara, Turkey.
Mullins M.G., Bouquet A. and Williams L.E. 1996. Biology of the Grapevine. 3rd edn. Cambridge University Press, Cambridge, United Kingdom.
Moore M.O. 1991. Classification and systematics of eastern North American Vitis L. (Vitaceae) north of Mexico. Sida 14: 339–367.
Moore M.O. (ed.) Flora of North America. Oxford Univ. Press, Oxford, United Kingdom. (in press).
Munson T.V. 1909. Foundations of American Grape Culture. T.V. Munson & Son, Denison, T.X.
Pavek D.S., Lamboy W.F. and Garvey E.J. 2001. In situ conservation America' wild grapes. HortScience 36: 232–235.
Prescott-;Allen R. and Prescott-;Allen C. 1984. Park your genes: Protected areas as in situ genebanks for the maintenance of wild genetic resources. In: McNeely J.A. and Miller K.R. (eds), National Parks, Conservation, and Development: The Role of Protected Areas in Sustaining Society. Smithsonian Institution Press, Washington, D.C., pp. 634–638.
Reisch B.I. and Pratt C. 1996. Grapes. In: Janick J. and Moore J.N. (eds), Fruit Breeding: Vine and Small Fruits CropsVol. II. Wiley, New York, pp. 297–396.
SAS Institute Inci 1997. SAS/STAT Software: Changes and Enhancements through Release 6.12. Cary, N.C.
Scheiner S.M. 1993. Genetics and evolution of phenotypic plasticity. Annu. Rev. Ecol. Syst. 24: 35–68.
Silvestroni O., Di Pietro D., Intrieri C., Vignani R., Filippetti I., Del Casino C. et al. 1997. Detection of genetic diversity among clones of cv. Fortana (Vitis vinifera L.) by microsatellite DNA polymorphism analysis. Vitis 36: 147–150.
Thomas M.R., Cain P. and Scott N.S. 1994. DNA typing of grapevines: A universal methodology and database for describtissue ing cultivars and evaluating genetic relatedness. Plant Mol. Biol. 25: 939–949.
Thomas M.R. and Scott N.S. 1993. Microsatellite repeats in grapevine reveal DNA polymorphisms when analyzed as se-;quence-;tagged sites (STSs). Theor. Appl. Genet. 86: 985–990.
USDA-;ARS National Genetic Resources Program. 2000. Germplasm Resources Information Network—GRIN. Online database. National Germplasm Resources Laboratory, Beltsville, MD, 16 June 2000, http://www.ars-;grin.gov/.
USDI National Park Service. 1994. Federal Native Plant Conservagenetiction Memorandum of Understanding. Online document. NPS Natural Resources Nature Net, Washington, D.C., 16 June 2000, http://www.nps.gov/ plants /mou.htm.
Wang X.-;D., Wang Z.-;P. and Zou Y.-;P. 1996. An improved procedure for the isolation of nuclear DNA from leaves of wild grapevine dried with silica gel. Plant Mol. Biol. Rptr. 14: 369–373.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pavek, D.S., Lamboy, W.F. & Garvey, E.J. Selecting in situ conservation sites for grape genetic resources in the USA. Genetic Resources and Crop Evolution 50, 165–173 (2003). https://doi.org/10.1023/A:1022947605916
Issue Date:
DOI: https://doi.org/10.1023/A:1022947605916