Abstract
The survival of neurons is a key condition for complete posttraumatic regeneration of the peripheral nerve. In experiments on rats we studied survival capacity of different neuronal subpopulations in LIV-LV dorsal root ganglia after ligation or transection and suturing of the sciatic nerve. Experiments with nerve ligation showed that IB4+ neurons are more sensitive to the injury than NF200+ neurons. By day 90 after ligation of the sciatic nerve IB4+ neurons were virtually not detected in the dorsal root ganglia. By day 90 after nerve transection the number of surviving NF200+ and IB4+ neurons decreased by 26.1 and 21.4%, respectively, in comparison with intact animals. Treatment with xymedon, a regeneration stimulator, led to a 48.5% increase in the number of surviving NF200+ neurons by day 30 after ligation of the nerve and a 50.7% increase by day 90. The number of surviving IB4+ neurons increased more than 8-fold by this term after ligation of the nerve and drug stimulation. Xymedon had a neuroprotective effect towards both neuron subpopulations, more intensely preventing apoptosis of IB4+ neurons.
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REFERENCES
I. S. Raginov, R. Kh. Khafiz'yanova, A. Yu. Vafin, and Yu. A. Chelyshev, Ros. Morfol. Vedomosti, 1,No. 6, 120–126 (1997).
I. S. Raginov and Yu. A. Chelyshev, Morfologiya, 118,No. 6, 36–40 (2000).
I. S. Raginov, Yu. A. Chelyshev, and T. F. Shagidullin, Byull. Eksp. Biol. Med., 131,No. 3, 275–277 (2001).
H. Hammarberg, F. Piehl, S. Cullheim, et al., Neuroreport, 7, 857–860 (1996).
D. Henken, W. Battisti, and M. Chesselet, Neuroscience, 39,No. 3, 733–742 (1990).
Y. Jiang, J. Pickett, and M. Oblinger, Brain Res., 637, 233–241 (1994).
P. Leclere, P. Ekstrom, A. Edstrom, et al., Neuroscience, 82, 545–558 (1997).
Q. Ma, Neuroreport, 12,No. 17, 3693–3695 (2001).
D. Molliver, D. Wright, M. Leitner, et al., Neuron, 19, 4849–4861 (1997).
V. Montpetit, D. Clapin, L. Tryphonas, and S. Dancea, Acta Neuropathol., 76,No. 1, 71–81 (1988).
J. Schionning, J. Larsen, T. Tandrup, and H. Braendgaard, Ibid., 96,No. 2, 191–201 (1998).
J. Silverman and L. Kruger, J. Neurocytol., 19, 789–801 (1990).
T. Tandrup, C. Woolf, and R. Coggeshall, J. Comp. Neurol., 422,No. 2, 172–180 (2000).
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Chelyshev, Y.A., Raginov, I.S. Effect of Stimulation of Nerve Regeneration on Posttraumatic Neuronal Survival in Dorsal Root Ganglia. Bulletin of Experimental Biology and Medicine 134, 597–599 (2002). https://doi.org/10.1023/A:1022929632535
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DOI: https://doi.org/10.1023/A:1022929632535