Abstract
Purpose: The objective was to establish the parameters for reversible electroporation of murine embryos.
Methods: In Trial 1, murine presumptive zygotes received an electrical pulse of 5, 10, or 20-μs duration, and one of five voltages (100, 200, 250, 300, or 400 V). In Trial 2, embryo orientation within the electroporation chamber was evaluated with 250 or 400 V at a pulse period of 10 μs.
Results: Presumptive zygotes that received 400 V at each pulse length and zygotes exposed to 20 μs at each voltage had the lowest embryonic development (P < 0.05). Presumptive zygotes that received 250 V had higher development compared to 400 V, irrespective of orientation (P < 0.01), but development was lower than the controls (P < 0.01).
Conclusions: Electrical stimulation of presumptive zygotes can have a detrimental impact on early embryo development, but low amounts of stimulation may allow for potential gene transfer in transgenic experimentation.
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Schmotzer, C.A., Dunlap-Brown, M.E., Butler, S.P. et al. Development of Murine Embryos Following Electroporation. J Assist Reprod Genet 20, 148–152 (2003). https://doi.org/10.1023/A:1022929119792
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DOI: https://doi.org/10.1023/A:1022929119792