Abstract
Exocytosis in pheochromocytoma cells was induced by electric stimulation. To chase the movement of vesicles by electric stimulation, dense-core secretory vesicles were visualized by expression of the fusion protein between neuropeptide Y and enhanced green fluorescent protein (EGFP) in these differentiated PC12 rat pheochromocytoma cells. When the cells were stimulated with constant voltage potential at −300 mV, the movement of dense-core secretory vesicles could be regulated.
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Mizuno, A., Mie, M., Yanagida, Y. et al. The secretory response through electric stimulation of differentiated PC12 rat pheochromocytoma cells transfected with neuropeptide Y fused with enhanced green fluorescent protein. Biotechnology Letters 25, 547–552 (2003). https://doi.org/10.1023/A:1022894220755
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DOI: https://doi.org/10.1023/A:1022894220755