Abstract
The histological structure of lucerne stems is a limiting factor for digestibility of the forage. Two experiments were carried out to compare cultivars and genotypes within cultivars for histological traits of the stems. In the first one, the among-cultivar genetic differences were assessed on 6 cultivars differing indigestibility. In the second one, the within-cultivar differences were evaluated on twenty genotypes belonging to two cultivars. Two sets of quantitative histological traits were measured using image analysis on the basal portion of the stems corresponding to the mature zone: the proportions of the different tissues of the lucerne stems (cortex, xylem, pithparenchyma), and the traits describing the xylem in vascular bundles (xylem cell wall thickness, xylem cell wall surface density, mean area of xylem vessel and mean area of fibre lumen). Few significant differences were observed among-cultivars, where as numerous within-cultivar differences were observed for histological traits. When the stem length was introduced as covariate, the differences among- and within-cultivars remained significant. Xylem proportion was negatively correlated to pith parenchyma proportion only. Xylem cell wall surface density and thickness were correlated to the cross-section radius but not to xylem proportion. As digestibility of lucerne is limited by histological structures, the large within-cultivar differences for histological traits of lucerne stems could be used in a breeding program for improved digestibility.
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References
Akin, D.E. & E.L. Robinson, 1982. Structure of leaves and stems of arrowleaf and Crimson clovers as related to in vitro digestibility. Crop Sci. 22: 24-29.
Bolaños-Aguilar, E.D., C. Huyghe, B. Julier & C. Ecalle, 2000. Genetic variation for seed yield and its components in alfalfa (Medicago sativa L.) populations. Agronomie 20: 333-345.
Buxton, D.R., J.S. Hornstein, W.F. Wedin & G.C. Marten, 1985. Forage quality in stratified canopies of alfalfa, Birdsfoot trefoil, and Red clover. Crop Sci 25: 273-279.
Crochemore, M.L., C. Huyghe, C. Ecalle & B. Julier, 1996. Partitioning and distribution of RAPD variation in a set of populations of the Medicago sativa complex. Agronomie 16: 421-432.
Jewett, J.G. & D.K. Barnes, 1992. Variability for stem anatomy among entries in the perennial medicago core collection. Report of the thirty-third North American Alfalfa Improvement Conference (NAAIC), 40. Atlanta, Georgia, June 14-18.
Jewett, J.G. & D.K. Barnes, 1994. Inheritance and potential utility of alfalfa stem anatomy traits. University of Guelph, Ontario, Canada. Report of the thirty-fourth North American Alfalfa Improvement Conference, 134.
Julier, B., C. Huyghe & C. Ecalle, 2000. Within-and among-cultivar genetic variation in alfalfa: forage quality, morphology, and yield. Crop Sci 40: 365-369.
Julier, B., C. Huyghe & C. Ecalle, 1999. Genetic variation and variety × environment interaction for digestibility, forage yield and protein content in alfalfa. Proceedings of the XIII Eucarpia Medicago ssp. Group Meeting, Perugia, Italy, 13-16 September 1999, pp. 359-364.
Jung, H.G. & D.A. Deetz, 1993. Cell wall lignification and degradability. In: H.G. Jung, D.R. Buxton, R.D. Hatfield & J. Ralph (Eds.), Forage Cell Wall Structure and Digestibility, pp. 315-346. Madison, WI (53711), USA, ASA-CSSA-SSSA.
Jung, H.G. & F.M. Engels, 2002. Alfalfa stem tissues: cell wall deposition, composition and degradability. Crop Sci 42: 524-534.
Kühbauch, W. & J. Bestajovsky, 1985. Determination of lignin concentration and digestibility in stem tissue of alfalfa by means of quantitative image analysis. Proc XVth Int Grassl Cong, pp. 825-826.
Lemaire, G. & J.M. Allirand, 1993. Relation entre croissance et qualité de la luzerne: interaction génotype-mode d'exploitation. Fourrages 134: 183-198.
Lenssen, A.W., E.L. Sorensen, G.L. Posler & L.H. Harbers, 1988. Forage quality of perrenial glandular-haired and eglandular Medicago populations. Crop Sci 28: 171.
Media Cybernetics, 1996. L.P, Optimas Corporation: version 6.1, eighth edition.
Morrison, T.A., J.R. Kessler & D.R. Buxton, 1994. Maize internode elongation patterns. Crop Sci 34: 1055-1060.
Piquemal, J.C., C. Lapierre, K. Myton, A. O'Connel, W. Schuch, J. Grima-Pettenati & A.M. Boudet, 1998. Down-regulation of cinnamyl-CoA reductase induces significant changes of lignin profiles in transgenic tobacco plants. Plant J 13: 71-83.
SAS Institute Inc, 1988. SAS: STAT user's guide, release 6.03 edition. SAS Institute Inc.: Carry, NC. USA.
Shenk, J.S. & F.C. Elliot, 1971. Plant compositional changes resulting from two cycles of directional selection for nutritive value in alfalfa. Crop Sci 11: 521-524.
Tolivia, D. & J. Tolivia, 1987. Fasga: a new polychromatic method for simultaneous and differential staining of plant tissue. J Microscopy 148: 113-117.
Wilson, J.R., 1993. Organization of plant tissues. In: H.G. Jung, D.R. Buxton, R.D. Hatfield & J. Ralph (Eds.), Forage Cell Wall Structure and Digestibility, pp. 1-32. Madison, WI (53711), USA, ASA-CSSA-SSSA.
Wilson, J.R. & R.D. Hatfield, 1997. Structural and chemical changes of cell wall types during stem development: consequences for fibre degradation by rumen microflora. Austr J Agric Sci 48: 165-180.
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Guines, F., Julier, B., Ecalle, C. et al. Among and within-cultivar variability for histological traits of lucerne (Medicago sativa L.) stem. Euphytica 130, 293–301 (2003). https://doi.org/10.1023/A:1022885320517
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DOI: https://doi.org/10.1023/A:1022885320517