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Dynamic tests and FSH biological activity in female rats with acute nephrotic syndrome

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Abstract

To investigate the pituitary-ovarian status during the acute state of the nephrotic syndrome, a sequence of experiments were undertaken in adult female rats after a single dose of the puromycin aminonucleoside (PAN). The functional condition of the hypophyseal-ovarian unit was determined in control and nephrotic rats by two dynamic tests. In the first one, 10 days after PAN or placebo administration female rats were stimulated with LHRH (300 ng/100 g body wt) and samples were collected at 0, 20, 40, 60 and 80 min after releasing factor administration. The second dynamic test, which was performed in control and nephrotic rats, consists of one (day 10 after PAN) or four (between days 7–10 after PAN) doses of hCG (8 UI), respectively. In all cases, serum samples were collected on day 10. In addition, the relative in vitro biological activity of FSH from control and nephrotic rats before and after LHRH stimulus was determined. The results reveal that after a stimulatory dose of LHRH the secretion of LH was significantly diminished in nephrotic rats at all registered times. By contrast, normal response was observed in terms of FSH secretion in nephrotic females. On the other hand, no ovarian response, in terms of progesterone or estradiol synthesis, was observed in nephrotic rats after either one or four stimuli with hCG. Interestingly, in spite of the normal or high concentrations of FSH, the biological activity of FSH was totally abolished in nephrotic rats. On the whole, the results from this study indicate that the nephrotic syndrome had a harmful effect on the pituitary-ovarian unit, and strongly suggest that the endocrine dysfunction could be initiated at the hypophysial level; even though a specific ovarian damage is also predictable.

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References

  1. Haack D, Scharer K, Asam-Taucher A, Vecsei P: Glucocorticoid receptor in idiopathic nephrotic syndrome. Pediatr Nephrol 13: 653-656, 1999

    Google Scholar 

  2. Ito S, Kano K, Ando T, Ichimura T: Thyroid function in children with nephrotic syndrome. Pediatr Nephrol 8: 412-415, 1994

    Google Scholar 

  3. Holdsworth S, Atkins RC, de Kretser DM: The pituitary-testicular axis in men with chronic renal failure. N Engl J Med 296: 1245-1249, 1977

    Google Scholar 

  4. Handelsman DJ, Spaliviero JA, Turtle JR: Hypothalamic-pituitary function in experimental uremic hypogonadism. Endocrinology 117: 1984-1995, 1985

    Google Scholar 

  5. Ramirez G, Butcher D, Brueggemeyer CD, Ganguly A: Testicular defect: The primary abnormality in gonadal dysfunction of uremia. South Med J 80: 698-701, 1987

    Google Scholar 

  6. Menjívar M, Vilchis F, Cárdenas M, Cruz C, Merchant H, Pérez-Palacios G, Pedraza-Chaverrí J: Pituitary-ovarian dysfunction in rats with induced nephrotic syndrome. Eur J Endocrinol 132: 502-506, 1995

    Google Scholar 

  7. Menjívar M, Vilchis F, Cárdenas M, Cruz C, Pérez-Palacios G, Pedraza-Chaverrí J: Transient alteration of the reproductive function in nephrotic rats. Life Sci 61: 1159-1167, 1997

    Google Scholar 

  8. Ortiz G, Vilchis F, Cárdenas M, Cruz C, Pedraza-Chaverrí J, Menjívar M: Reproductive function in male rats with chronic nephrosis. J Reprod Fertil 117: 223-228, 1999

    Google Scholar 

  9. Menjívar M, Cárdenas M, Ortiz G, Pedraza-Chaverrí J: Fertility diminution in female rats with experimental chronic nephrosis. Biol Reprod 63: 1559-1564, 2000

    Google Scholar 

  10. Menjívar M, Ortiz-López MG, Vilchis F, Díaz-Bonilla L, Zambrano E, Zariñán T, Pedraza-Chaverrí J: Evaluation of the pituitary-testicular function during experimental nephrosis. Life Sci 70: 2769-2782, 2002

    Google Scholar 

  11. Menjívar M, Ortiz G, Vilchis F, Pedraza-Chaverrí J, Larrea F, Correa-Rotter R: Pituitary-testicular axis alterations in men with nephrotic syndrome. In: Program of 81st Annual Meeting of the Endocrine Society, 1999, San Diego, CA. Abstract P2–435

  12. Lewandowski AE, Liao WSL, Stinson-Fisher CA, Kent JD, Jeferson LS: Effect of experimentally induced nephrosis on protein synthesis in rat liver. Am J Physiol 254: C634-C642, 1988

    Google Scholar 

  13. Fox RR: Sexual cycles. In: E.S.E. Hafez (ed). Reproduction and Breeding Techniques of Laboratory Animals. Lea and Febiger; Philadelphia, 1970, pp 115-125

    Google Scholar 

  14. Freeman ME: The neuroendocrine control of the ovarian cycle of the rat. In: E. Knobil, J.D. Neill, G.S. Greenwald, L.M. Clement, W.P. Donald (eds). The Physiology of Reproduction, vol. 2, 2nd edn. Raven Press, New York, 1994, pp 613-658

    Google Scholar 

  15. Pedraza-Chaverrí J, Calderón P, Cruz C, Peña JC: Electrophoretic analysis of serum and urinary proteins in rats with aminonucleoside-induced nephrotic syndrome. Ren Fail 15: 149-155, 1981

    Google Scholar 

  16. Tilly JL, Aihara T, Nishimori K: Expression of recombinant human follicle-stimulating hormone receptor: Species-specific ligand binding signal transduction, and identification of multiple ovarian messenger ribonucleic acid transcripts. Endocrinology 131: 799-806, 1992

    Google Scholar 

  17. Zambrano E, Barrios-de-Tomasi J, Cárdenas M, Ulloa-Aguirre A: Studies on the relative in vitro biological potency of the naturally-occurring isoforms of intrapituitary follicle stimulating hormone. Mol Hum Reprod 2: 563-571, 1996

    Google Scholar 

  18. Zariñán T, Olivares A, Söderlund, Méndez JP, Ulloa-Aguirre A: Changes in the biological:immunological ratio of basal and GnRH-releasable FSH during the follicular, pre-ovulatory and luteal phases of the human menstrual cycle. Hum Reprod 16: 1611-1618, 2001

    Google Scholar 

  19. Themmen APN, Huhtaniemi I: Mutations of gonadotropins and gonadotropin receptors: Elucidating the physiology and pathophysiology of pituitary-gonadal function. Endocr Rev 21: 551-583, 2000

    Google Scholar 

  20. McCann SM, Marabayashi U, Sun H-Q, Yu WH: Control of follicle stimulating hormone and luteinizing hormone release by hypothalamic peptides. In: GP. Chrousos, G. Tolis (eds). Intraovarian Regulators and Polycystic Ovarian Syndrome: Recent Progress on Clinical and Therapeutic Aspects. Ann NY Acad Sci, New York, 1993, pp 687-655

    Google Scholar 

  21. Culler MD, Negro-Vilar A: Pulsatile follicle-stimulating hormone (LHRH): Pulsatile replacement of LHRH bioactivity in LHRH-immuno neutralized rats: Endocrinology 120: 2011-2021, 1987

    Google Scholar 

  22. Flack MR, Froehlich J, Bennet AP, Anasti J, Nisula BC: Site-directed mutagenesis defines the individual roles of the glycosylation sites on follicle-stimulating hormone. J Biol Chem 269: 14015-14020, 1994

    Google Scholar 

  23. Wicking EJ, Hillier SG, Reichert LE Jr: Gonadotropin control of steroidogenesis in human granulosa-lutein cells. J Reprod Fertil 76: 677-684, 1986

    Google Scholar 

  24. Steinberg HO, Baron AD: Vascular function, insulin resistance and fatty acids. Diabetologia 45: 623-634, 2002

    Google Scholar 

  25. Simpson ER, Mahendroo MS, Means GD, Kilgore MW, Corbin CJ, Mendelson CR: Tissue-specific promoters regulate aromatase cytochrome P450 expression. Clin Chem 39: 317-324, 1993

    Google Scholar 

  26. James VH, Few JD: Adrenocorticosteroids: Chemistry, synthesis and disturbances in disease. Clin Endocrinol Metab 14: 867-892, 1985

    Google Scholar 

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Authors and Affiliations

  1. Department of Reproductive Biology, Hospital Juárez de México, Mexico

    María Guadalupe Ortiz-López

  2. Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico

    Mario Cárdenas & Elena Zambrano

  3. Research Unit in Reproductive Medicine, Hospital de Ginecobstetricia No 4 ‘Luis Castelazo Ayala’, IMSS, Mexico

    Teresa Zariñán

  4. Department of Biology, Faculty of Chemistry, Universidad Nacional Autónoma de México, Mexico City, Mexico

    Laura Díaz-Bonilla, José Pedraza-Chaverrí & Marta Menjívar

Authors
  1. María Guadalupe Ortiz-López
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  2. Mario Cárdenas
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  3. Teresa Zariñán
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  4. Laura Díaz-Bonilla
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  5. Elena Zambrano
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  6. José Pedraza-Chaverrí
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  7. Marta Menjívar
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Guadalupe Ortiz-López, M., Cárdenas, M., Zariñán, T. et al. Dynamic tests and FSH biological activity in female rats with acute nephrotic syndrome. Mol Cell Biochem 245, 167–172 (2003). https://doi.org/10.1023/A:1022880414635

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