Skip to main content
Log in

Plant Micropropagation and Callus Induction of Some Annual Salsola Species

  • Published:
Biologia Plantarum

Abstract

Micropropagated plants of two annual haloxerophytic Asiatic Salsola species (S. pestifer and S. lanata) were obtained from zygotic embryos cultured on Murashige and Skoog (MS) agar medium supplemented with 0.5 µM benzylamino-purine (BAP) and 0.3 µM indole-3-acetic acid (IAA) or with 0.5 µM 6 γ, γ-dimethylallylaminopurine and 0.3 μM IAA. The callus induction from shoot and leaf explants derived from plants propagated in vitro were obtained on MS agar medium with various concentration of auxins and cytokinins. The best medium for growth and proliferation of calluses of both studied species was MS medium containing 9.0 µM 2,4-dichlorophenoxyacetic acid. It was also determined that beginning of plant regeneration from callus of S. lanata was induced by 8.8 µM BAP.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Bulgakov, V.P., Juravlev, Y.: Callus culture induction of Aristolochia manshuriensis Kom.-Plant Res. 25: 266–270, 1989.

    Google Scholar 

  • Casstellar, M.R., Iborra, J.L.: Callus induction from explants of Crocus sativus.-J. Plant Biochem. Biotechnol. 6: 97–100, 1997.

    Google Scholar 

  • Elhaak, M.A.: Effect of abscisic acid on growth and certain osmoregulatory metabolites in the leaf callus of Gymnocarpos decandrum.-Arab Gulf J. Sci. Res. 17: 95–109, 1999.

    CAS  Google Scholar 

  • George, E. F.: Plant Propagation by Tissue Culture.-Exegetics Ltd., Edington 1993.

    Google Scholar 

  • Kenny, L., Caligari, P.: Androgenesis of salt tolerant shrub Atriplex glauca.-Plant Cell Rep. 15: 829–832, 1996.

    Article  CAS  Google Scholar 

  • Li, V.V.: The opportunities of using of annual Salsola species associated with rehabilitation of salt/affected lands in South-Eastern Kyzylkum.-In: Abstracts of the International Scientific Conference ”Problems of Desertification in Arid Zones”. P. 69. Uzbek Academy of Sciences, Samarkand 2000.

    Google Scholar 

  • Luo, J.P., Jia, J.F.: Callus induction and plant regeneration from hypocotyls explants of the forage legume Astragalus adsurgens.-Plant Cell Rep. 17: 567–570, 1998.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue culture.-Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Pyankov, V.I., Artyusheva, E.G., Edwards, G.E., Black, C.C., Jr., Soltis, P.S.: Phylogenetic analysis of tribe Salsoleae (Chenopodiaceae) based on ribosomal ITS sequences: implications for the evolution of phytosynthesis types.-Amer. J. Bot. 88: 1189–1198, 2001.

    CAS  Google Scholar 

  • Thiyagarajah, M., Fry, S.C., Yeo, A.R.: In vitro salt tolerance of cell wall enzymes from halophytes and glycophytes.-J. exp. Bot. 47: 1717–1724, 1996.

    CAS  Google Scholar 

  • Upadhyay, N., Makoveychuk, A.Y., Nikolaeva, L.A., Batygina, T.B.: Organogenesis and somatic embryogenesis in leaf callus culture of Rauwolfia caffra Sond.-J. Plant Physiol. 140: 218–222, 1992.

    CAS  Google Scholar 

  • Zheng, M.Y., Konzak, C.F.: Effect of 2,4–dichlorophenoxyacetic acid on callus induction and plant regeneration in anther culture of wheat (Triticum aestivum L.).-Plant Cell Rep. 19: 69–73, 1999.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Stefaniak, B., Woźny, A. & Li, V. Plant Micropropagation and Callus Induction of Some Annual Salsola Species. Biologia Plantarum 46, 305–308 (2003). https://doi.org/10.1023/A:1022879400747

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022879400747

Navigation