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Genetic analysis of the Aegilops longissima 3S chromosome carrying the Pm13 resistance gene

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Abstract

The distal region of the short arm of chromosome 3S from Aegilopslongissima, which carries the powdery mildew resistance gene Pm13, was introgressed into common wheat. Due to suppression of recombination between this region and corresponding wheat homoeologous segments, a possible strategy to construct a genetic map around the Pm13 gene was based on crosses between a wheat addition line carrying the Ae.longissima 3S chromosome and the corresponding 3S addition lines of Ae.searsii and Ae. variabilis. The efficiency of this strategy was evaluated by scoring recombination frequencies inprogenies derived from these crosses. Recombination between 3S chromosomes fromAe. searsii and Ae. longissimawas very low, whereas 26.5% recombinant alien chromosomes were obtained from the cross involving the Ae. variabilisand Ae. longissima 3S addition lines. These data were used to construct a3S chromosome map that resulted largely colinear to the consensus map of the homoeologous group 3 of wheat.

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Alberto, C., Renato, D., Oronzo Antonio, T. et al. Genetic analysis of the Aegilops longissima 3S chromosome carrying the Pm13 resistance gene. Euphytica 130, 177–183 (2003). https://doi.org/10.1023/A:1022873822114

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