Abstract
The distal region of the short arm of chromosome 3S from Aegilopslongissima, which carries the powdery mildew resistance gene Pm13, was introgressed into common wheat. Due to suppression of recombination between this region and corresponding wheat homoeologous segments, a possible strategy to construct a genetic map around the Pm13 gene was based on crosses between a wheat addition line carrying the Ae.longissima 3S chromosome and the corresponding 3S addition lines of Ae.searsii and Ae. variabilis. The efficiency of this strategy was evaluated by scoring recombination frequencies inprogenies derived from these crosses. Recombination between 3S chromosomes fromAe. searsii and Ae. longissimawas very low, whereas 26.5% recombinant alien chromosomes were obtained from the cross involving the Ae. variabilisand Ae. longissima 3S addition lines. These data were used to construct a3S chromosome map that resulted largely colinear to the consensus map of the homoeologous group 3 of wheat.
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Badaeva, E.D., B. Friebe, S.A. Zoshchuk, A.V. Zelenin & B.S. Gill, 1998. Genome differentiation in diploid and polyploid Aegilops species. In: A.E. Slinkard (Ed.), Proc 9th IntWheat Genet Symp, vol. 1, pp. 61-64. Saskatoon, Saskatchewan, Canada.
Biagetti, M., F. Vitellozzi & C. Ceoloni, 1999. Physical mapping of wheat-Ae. longissima breakpoints in mildew resistant recombinant lines using FISH with highly repeated and low-copy DNA probes. Genome 42: 1013-1019.
Brunner, S., B. Keller & C. Feuillet, 2000. Molecular mapping of the Rph7.g leaf rust resistance gene in barley (Hordeum vulgare L.). Theor Appl Genet 101: 783-788.
Cenci, A., R. D'Ovidio, O.A. Tanzarella, C. Ceoloni & E. Porceddu, 1999. Identification of molecular markers linked to Pm13, an Aegilops longissima gene conferring resistance to powdery mildew in wheat. Theor Appl Genet 98: 448-454.
Ceoloni, C., 1983. Triticum longissimum chromosome G ditelosomic addition lines: production, characterization and utilization. In: S. Sakamoto (Ed.), Proc 6th Int Wheat Genet Symp, pp. 1025-1031. Kyoto, Japan.
Ceoloni, C., 1987. Current methods of chromosome engineering in wheat. In: J. Sutka & A.J. Worland (Eds.), EWAC Newsletter, pp. 95-109.
Ceoloni, C., G. Del Signore, M. Pasquini & A. Testa, 1988. Transfer of mildew resistance from Triticum longissimum into wheat by ph1 induced homoeologous recombination. In: T.E. Miller & R.M.D. Koebner (Eds.), Proc 7th Int Wheat Genet Symp, pp. 221-226. Cambridge, UK.
Ceoloni, C., G. Del Signore, L. Ercoli & P. Donini, 1992. Locating the alien chromatin segment in common wheat-Aegilops longissima mildew resistant transfers. Hereditas 116: 239-245.
de la Peña, R.C., K.P. Smith, F. Capettini, G.J. Muehlbauer, M. Gallo-Meagher, R. Dill-Macky, D.A. Somers & D.C. Rasmusson, 1999. Quantitative trait loci associated with resistance to Fusarium head blight and kernel discoloration in barley. Theor Appl Genet 99: 561-569.
Dellaporta, S.L., J. Wood & J.B. Hicks, 1983. A plant DNA minipreparation: version II. Plant Mol Biol Reporter 1: 19-21.
Dingerdissen, A.L., H.H. Geiger, M. Lee, A. Schechert & H.G. Weltz, 1996. Interval mapping of genes for quantitative resistance of maize to Setesphaeria turcica, cause of northern leaf blight, in a tropical environment. Mol Breed 2: 143-156.
Donini, P., R.M.D. Koebner & C. Ceoloni, 1995. Cytogenetic and molecular mapping of the wheat-Aegilops longissima chromatin breakpoints in powdery mildew resistant introgression lines. Theor Appl Genet 91: 738-743.
D'Ovidio, R., O.A. Tanzarella, A. Cenci, E. Iacono & E. Porceddu, 1994. RFLP analysis in wheat. Isolation and chromosomal assignment of digoxigenin-labelled clones. J Genet Breed 48: 73-80.
Dvorák, J., 1998 Genome analysis on the Triticum-Aegilops alliance. In: A.E. Slinkard (Ed.), Proc 9th Int Wheat Genet Symp, vol. 1, pp. 8-11. Saskatoon, Saskatchewan, Canada.
Feldman, M., 1975. Alien addition lines of common wheat containing Triticum longissimum chromosomes. In: Proc XII Int. Bot Congr, p. 506.
Feuillet, C., G. Schachermayr & B. Keller, 1997. Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of wheat. Plant J 11: 45-52.
Friebe, B., N. Tuleen, J. Jiang & B.S. Gill, 1993. Standard karyotype of Triticum longissimum and its cytogenetic relationship with T. aestivum. Genome 36: 731-742.
Heun, M., A.E. Kennedy, J.A. Anderson, N.L.V. Lapitan, M.E. Sorrells & S.D. Tanksley, 1991. Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34: 437-447.
Keller, M., B. Keller, G. Schachermayr, M. Winzeler, J.E. Schmid, P. Stamp & M.M. Messmer, 1999. Quantitative trait loci for resistance against powdery mildew in a segregating wheat × spelt population. Theor Appl Genet 98: 903-912.
Liang, P. & A.B. Pardee, 1992. Differential display of Eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257: 967-971.
Nelson, J.C., R.P. Singh, J.E. Autrique & M.E. Sorrels, 1997. Mapping genes conferring and suppressing leaf rust resistance in wheat. Crop Sci 37: 1928-1935.
Pietro, M.E., N.A. Tuleen & G.E. Hart, 1988. Development of wheat-Triticum searsii disomic chromosome addition lines. In: T.E. Miller & R.M.D. Koebner (Eds.), Proc 7th Int Wheat Genet Symp, pp. 409-413. Cambridge, UK.
Qi, L.L., S.L. Wang, P.D. Chen, D.J. Liu & B.S. Gill, 1998. Identification and physical mapping of three Haynaldia villosa chromosome-6V deletion lines. Theor Appl Genet 97: 1042-1046.
Sears, E.R., 1973. Agropyron-wheat transfers induced by homoeologous pairing. In: Proc 4th Int Wheat Genet Symp, pp. 191-99. University of Missouri, Columbia, MO.
Sears, E.R., 1982. Transfer of alien genetic material to wheat. In: Evans & Peacock, (Eds.), Wheat Science-Today and Tomorrow, pp. 75-89. Cambridge Univ Press, England.
Shepherd, K.W. & S.K.M.R. Islam, 1988. Fourth compendium of wheat-alien chromosome lines. In: T.E. Miller & R.M.D. Koebner (Eds.), Proc 7th Int Wheat Genet Symp, pp. 1373-1395. Cambridge, England.
Tanksley, S.D., M.W. Ganal & G.B. Martin, 1995. Chromosome landing: a paradigm for map-based cloning in plants with large genomes. Trends Genet 11: 63-68.
Taylor, C., K.W. Shepherd & P. Langridge, 1998. A molecular genetic map of the long arm of chromosome 6R of rye incorporating the cereal cyst nematode resistance gene, CreR. Theor Appl Genet 97: 1000-1012.
Waldron, B.L., B. Moreno-Sevilla, J.A. Anderson, R.W. Stack & R.C. Frohberg, 1999. RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39: 805-811.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18(22): 6531-6535.
Yang, Y.-C., N.A. Tuleen & G.E. Hart, 1996. Isolation and identification of Triticum aestivum L. em. Thell. cv Chinese Spring-T. peregrinum Hackel disomic chromosome addition lines. Theor Appl Genet 92: 591-598.
Yildrim, A., S.S. Jones & T.D. Murray, 1998. Mapping a gene conferring resistance to Pseudocercosporella herpotrichoides on chromosome 4V of Dasypyrum villosum in a wheat background. Genome 41: 1-6.
Yu, M.Q. & J. Jahier, 1992. Origin of Sv genome of Aegilops variabilis and utilization of the Sv as analyser of the S genome of the Aegilops species in the Sitopsis section. Plant Breed 108: 290-295.
Zhang, H.-B., J. Dvorák & J.G. Waines, 1992. Diploid ancestry and evolution of Triticum kotschyi and T. peregrinum examined using variation in repeated nucleotide seguences. Genome 35: 182-191.
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Alberto, C., Renato, D., Oronzo Antonio, T. et al. Genetic analysis of the Aegilops longissima 3S chromosome carrying the Pm13 resistance gene. Euphytica 130, 177–183 (2003). https://doi.org/10.1023/A:1022873822114
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DOI: https://doi.org/10.1023/A:1022873822114